ACS Applied Materials & Interfaces
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2017
·
IF 7.504
Zhen Lei,Hongda Chen,Hua Zhang,Yaoqi Wang,Xianying Meng,Zhenxin Wang
Matrix metalloproteinases (MMPs) are important biomarkers and potential therapeutic targets of tumor. In this report, a peptide microarray-based fluorescence assay is developed for MMPs inhibitors evaluation through immobilization of biotin-modified peptides on the poly(glycidyl methacrylate-co-2-hydroxyethyl methacrylate) (P(GMA-HEMA)) brush-modified glass slides. After biotin is recognized with cyanine 3 (Cy3)-modified avidin (Cy3-avidin), the microarrays can produce strong fluorescence signal. The biotin moieties detach from microarray, when the biotin-modified peptide substrates are specially cleaved by a MMP, resulting in decreased fluorescence intensity of the microarray. The decreasing level of fluorescence intensity is correlated with the MMP inhibition. Nine known MMP inhibitors against MMP-2 and MMP-9 are evaluated by the assay, and the quantitative determination of inhibitory potencies (half maximal inhibitory concentration) are obtained, which are comparable with the literatures. Two biocompatible fluorogenic peptides containing MMP-specific recognition sequences and FAM/Dabcyl fluorophore-quencher pair are designed as activatable reporter probes for sensing MMP-2 and MMP-9 activities in cell and in vivo. The peptide microarray-based results are well verified by the cell inhibition assay and in vitro fluorescence imaging, and further confirmed by the in vivo imaging of HT-1080 tumor-bearing mice.
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CHEMISTRY-A EUROPEAN JOURNAL
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2023
·
IF 4.3
Yuan-Yang Guo,Ze-Hua Tian,Yu-Chen Han,Dandan Ma,Tianju Shao,Zhiyong Jiang
Graphical Chemically stable Hantzsch ester is discovered to be an efficient and economical NAD(P)H mimic as sacrificial electron source in in vitro bioconversions. Biocatalysis has emerged as a valuable and reliable tool for industrial and academic societies, particularly in fields related to bioredox reactions. The cost of cofactors, especially those needed to be replenished at stoichiometric amounts or more, is the chief economic concern for bioredox reactions. In this study, a readily accessible, inexpensive, and bench-stable Hantzsch ester is verified as the viable and efficient NAD(P)H mimic by four enzymatic redox transformations, including two non-heme diiron N -oxygenases and two flavin-dependent reductases. This finding provides the potential to significantly reduce the costs of NAD(P)H-relying bioredox reactions.
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Journal of Biomedical Nanotechnology
·
2018
·Vol. 14
·期 9
·1613-1626
·
IF 5.068
Xia Qingming,Gong Chunai,Gu Fenfen,Wang Zhuo,Hu Chuling,Zhang Lijuan,Qiang Lei,Ding Xueying,Gao Shen,Gao Yuan
Immuno-based oncotherapy has been successfully implemented for cancer treatment. In the present study, we developed a Oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs (CpG ODNs) nano-delivery system based on Multi-walled carbon nanotubes (MWCNTs) conjugated with H3R6
polypeptide (MHR-CpG) for prostate cancer immunotherapy. The in vitro and in vivo toxicity data revealed that the prepared MHR showed high biocompatibility. Confocal laser scanning microscopy confirmed that MHR-CpG could specifically target the endosomal TLR9. In addition, the
use of MHR enhanced the immunogenicity of CpG in both humoral and cellular immune pathways, as evidenced by the increased expression of CD4+ T-cells, CD8+ T-cells, TNF-α, and IL-6. The in vivo anti-cancer efficacy study on RM-1 tumor-bearing mice demonstrated
that MHR-CpG could deliver the immunotherapeutics to the tumor site and the tumor-draining lymph node to suppress tumor growth. These results suggested that MHR-CpG was a promising multifunctional nano system for prostate cancer immunotherapy.
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TALANTA
·
2011
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IF 3.722
Fang Li,Rui Zhou,Kaihong Zhao,Huanchun Chen,Yonggang Hu
A novel magnetic electrochemical immunosensor has been developed for the detection of pseudorabies virus antibody in swine serum. The magnetic glass carbon electrode was fabricated to manipulate magnetic beads for the direct sensing applications. Magnetic beads were employed as the platforms for the immobilization and immunoreaction process, and gold nanoparticles were chosen as electroactive labels for the electrochemical detection. The parameters concerning the assay strategy were carefully investigated. Under the optimal conditions, the linear response range of pseudorabies virus antibody dilution ratio (standard positive serum) was 1:250 to 1:1000 with a detection limit of 1:1000. Finally, this developed immunoassay method was successfully applied in the detection of pseudorabies virus antibody in swine serum, and had a good diagnostic accordance in comparison with ELISA.
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COLORATION TECHNOLOGY
·
2023
·
IF 1.8
Kangwei Si,Zhiwei Xu,Xiangdong Zhou
A series of vegetable oil-based nonionic Gemini surfactant (GPNGS) was prepared using grape seed oil and polyethylene glycol monomethyl ether (350, 550, 750) as the primary raw materials. The chemical structures of different types of GPNGS were characterised by Fourier Transform–infrared spectroscopy, elemental analysis, proton nuclear magnetic resonance and carbon-13 nuclear magnetic resonance. The critical micelle concentration (CMC) of GPNGS with different polyoxyethylene chain lengths was measured using the Wilhelmy ring method (CMC: ca . 1.4 × 10 −4 to 5 × 10 −4 mol/L). The results of wettability testing showed that the contact angle of GPNGS on polyamide film was ca . 46-49°. In addition, the interaction of surfactants with acid dyes was also investigated. The ultraviolet spectrum showed a balance between dyes, surfactants and the dye/surfactant complex. Next, the GPNGS was used as a nylon 66 acid dye levelling agent. After adding 0.2 mmol/L of surfactants, the standard deviations (S(λ)) of nylon 66 dyeing decreased from 0.084 to ca . 0.036-0.044, exhibiting a good levelling effect.
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PHYTOMEDICINE
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2021
·
IF 5.34
Zheng Niu,Min Fu,Yuan Li,Huanhuan Ren,Xuanyu Zhang,Li Yao
Background Pulmonary vascular remodeling is the key pathological feature of pulmonary arterial hypertension (PAH) characterized by a pattern of lipid-related insulin resistance(IR), hormonal derangements and metabolic reprogramming. Our previous studies have demonstrated osthole as natural coumarin compound derived from traditional Chinese medicine is a promising agent for the treatment of pulmonary vascular remodeling in PAH. Purpose The present study sought to delineate lipid metabolic modulatory mechanism of osthole against pulmonary vascular remodeling by employing an interdisciplinary strategy. Methods Rat model with PAH induced with MCT and PASMCs proliferation model induced with PDGF-BB were established in this study. Serum and lung tissues were used to lipid-related IR, hormone related indexes, pulmonary vascular remodeling analysis. Then, lipid metabolic gene, key enzymes , metabolites and cell proliferation indexes were examined to investigate metabolic regulatory mechanism in vivo and vitro model of PAH. Results Osthole significantly showed improvement of lipid-related IR and hormone dysregulation in rats with PAH evidenced by elevating testosterone, androgen receptor and cyclic guanosine monophosphate (cGMP), inhibiting phosphodiesterase-5(PDE-5), modulating lipid-related IR indexes total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), triglyceride (TG)/HDL-C ratio. Additionally, osthole limited key metabolic gene and enzymes to inhibit accumulation of decadienyl-l-carnitine in lipid metabolism , thus to promote oxidative phosphorylation and ATP production through inhibition of miRNA-22–3p, fatty acid translocase (CD36), fatty acid synthase (FAS), phospholipase A2 (PLA2), carnitine palmitoyltransferase 1A (CPT1A), hexokinase 2 (HK2), activation of metabolic switch isocitrate dehydrogenase 3α (IDH3α), NADH dehydrogenase 1 (ND1). We found for the first time miRNA-22–3p modulated PASMCs proliferation and vascular remodeling by regulating lipid metabolism reprogramming. Those modifications uncovered therapeutic mechanism of osthole against pulmonary vascular remodeling. Conclusion Our findings revealed the function of miRNA-22–3p in PASMCs and demonstrated a novel mechanism that miRNA-22–3p as a regulator can be targeted by osthole to greatly restore dysregulated lipid metabolism thus to alleviate pulmonary vascular remodeling in PAH, which provides novel insight into the potential therapeutic target for PAH, further highlights the development potential of osthole derived new drug against PAH.
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Applied Materials Today
·
2021
·
IF 10.041
Mengjiao Ma,Guodong Liu,Haoye Meng,Yudong Zheng,Jiang Peng,Yajie Xie,Yansen Wang,Wei He,Shuai Wei
In regenerating and repairing the nerve injured by the congenital malformation or trauma, the conundrum of electrical signal transmission between the nerve cells in damaged nerve tissue remains an uncrossable barrier, owing to potential limitations of the tissue in repair. Filling conductive guiding cues into nerve conduits has been effective in stimulating functional nerve recovery. The present study presents a novel porous conductive chitosan/polydopamine (CS/PDA) microcarriers for nerve defect repair were prepared by the self-polymerization properties of mussel-like macromolecule dopamine. The porosity is more than 90%, and the electrical conductivity reaches 7 × 10 −4 S/cm. The electrical signal was simulated by electrical stimulation in vitro . Compared with non-conductive microcarriers, conductive ones can effectively promote cell adhesion and proliferation. The conductive microcarriers loaded rat adipose-derived stem cells (rADSCs) were injected into nerve conduits and then transplanted into SD rats, with the autologous and the hollow groups as controls. The results of histology, immunohistochemical staining and gait analysis showed that CS/PDA had a promising ability to repair nerve defects in vivo .
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2020
·
IF 3.99
Chengyong Gao,Yue Zhang,Shipan Mia,Tieling Xing,Guoqiang Chen
In textile inkjet printing, it is important to understand the design theory of ink jet printing ink. In this study, according to the solubility parameters of printing ink components (humectant, penetrant, pH regulator, viscosity regulator and deionized water), twelve different complex solvents were designed to prepare disperse ink. By observing the particle size, stratification, rheological property and viscosity of the prepared inks, the ink meeting the requirements was subsequently selected. The low field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) analyses were used to analyze the water distribution and structure information of the ink at the micro level, which provide opportunities to subsequently interpret the macro properties (fluidity) of ink. The ink before and after filtration was tested using an ultraviolet-visible spectrophotometer (UV), and the content of the disperse dye in the ink was observed to be reduced after filtration. The viscosity of the ink before and after filtration were also analyzed. The viscosity of the ink was noted to significantly decrease after filtration, which indicated that the content of the disperse dye in the ink exhibited a vital influence on the ink viscosity. LF-NMR was applied to study the stability of ink (1a, 1c, 3a, 3c, 4a, 4c, 5a, 5c, 6a and 6c) in cold and hot environments. No new peak was found in T 2 relaxation time inversion spectra of disperse ink, which indicates that there is no physical or chemical change among the components in disperse ink. The voltage of the driving waveform was adjusted and ejection performance of different inks was characterized. The results indicated linear driving voltage and ligament length of the inks. Different fixation temperatures (160 ℃-190 ℃) were used to optimize the fixation temperature of the printed fabrics, and 180 ℃ was determined to be the optimal fixation condition. Finally, the color fastness of the printed fabric was tested and analyzed. The findings revealed that the color fastness of the printed fabric met the use requirements.
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FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Huili Hao,Manqin Fu,Ru Yan,Baolin He,Meiying Li,Qiabiao Liu,Yimian Cai,Xiaoyong Zhang,Riming Huang
This work aimed to evaluate the nutritional composition and immunostimulatory activity of an edible green alga Caulerpa racemosa var peltata collected from South China Sea. The results indicated that this alga contained the total carbohydrates (71.67%, DW), crude protein (11.39%, DW), and crude lipid (1.03%, DW), and was rich in taurine (7.71 mg/100 g, DW), essential amino acids (40.07%), monounsaturated fatty acids (25.72%), and polyunsaturated fatty acids (26.33%). Moreover, water extraction of this alga (WEC) increased NO, ROS, TNF-α, and IL-6 in a dose-dependent manner in RAW264.7 macrophage cells. Furthermore, WEC contained the total sugar (34.19 ± 0.46 mg/g), total protein (6.76 ± 0.32 mg/g), total phenolic (1.93 ± 0.04 mg/g), and total flavonoid (4.80 ± 0.10 mg/g). In addition, the MSn analysis of WEC indicated that the presence of 19 compounds. Thus, these findings suggest that this alga may have a potential nutraceutical value.
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ACS Applied Nano Materials
·
2023
·
IF 5.9
Yu Yang,Hao Cheng,Ruijue Chen,Qiying Chen,Si Gao,Li Chen,Wenyi Huang,Hongxing Kong,Lijun Li,Jun Feng
In this work, we have fabricated a surface-enhanced Raman scattering (SERS) magnetic immunosensor using a sandwich structure consisting of an SERS tag, a supporting substrate with magnetic properties, and a target. On the one hand, the SERS tag consists of AgNPs, a metal–organic framework called the zeolite imidazolate framework-67 (ZIF-67), and R6G, which acts as a Raman reporter. Due to the self-selective SERS-enhancing properties of ZIF-67 and better adsorption to analytes, SERS tags with this structure can further enhance the sensing capabilities. On the other hand, after surface functionalization with the human carboxylesterase 1 (hCE1) antibody, the nanostructure containing CoFe2O4 as the magnetic core and Ag NPs as the shell was used as the magnetic support substrate. The introduction of the CoFe2O4 magnetic core can not only realize the rapid separation of biological samples to simplify pre-treatment procedures but also stabilize and uniformly distribute the AgNPs and increase the Raman “hot spots”, thereby further enhancing the SERS intensity and improving the reproducibility. Not only that, the SERS signal can be further enhanced by the synergistic effect generated by the plasmonic coupling of the CoFe2O4 magnetic core–Ag satellite nanostructure (CoFe2O4/Ag). hCE1 in HepG-2 cell lysates and culture supernatants was rapidly determined using the constructed SERS magnetic immunosensor, and hCE1 released from acetaminophen-treated HepG-2 cells into the extracellular medium was also monitored in real time. The limit of detection was as low as 3.6 pg/mL, and the linear response covered a range of hCE1 concentrations (1.0 ng/mL–1.0 mg/mL) spanning 3 orders of magnitude. This strategy can be used for the quantitative detection of complex trace proteins or enzymes in biological samples.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2020
·Vol. 22
·期 9
·1-14
·
IF 2.132
Zhang Jun-Zheng,Tang Huan,Chen Xing-Zhu,Su Qianqian,Xi Wen-Song,Liu Yuan-Yuan,Liu Yuanfang,Cao Aoneng,Wang Haifang
Narrow bandgap Ag 2 Te quantum dot (QDs) hold great promise in the fields of bioimaging, chemo-/bio-detection, and thermoelectric materials, but their biosafety has not been well estimated yet. In this study, we studied the biodistribution and toxicity of polyethylene glycol–coated Ag 2 Te QDs (Ag 2 Te QDs-PEG) in mice after a single intravenous injection. After entering the mice, Ag 2 Te QDs-PEG displayed a short circulation time in the blood (half-life was less than 2 h), and mainly accumulated in the liver and spleen. The stability of Ag 2 Te QDs-PEG in the mice depended on accumulation organ and time. Ag 2 Te QDs-PEG were stable in the spleen within 28 days but decomposed in the liver after 7 days. The decomposed Ag 2 Te QDs-PEG were eliminated from the body via different routes, as Ag was detected only in feces while Te was found only in urine. Meanwhile, Ag 2 Te QDs-PEG did not show obvious adverse effects on the body weights, organ indices, biochemical parameters, the levels of essential metal Cu and Zn in organs, and organ pathological investigations during 28 days. In addition, the important effects of anion to the fates of silver chalcogenide QDs in vivo was demonstrated by comparing the distributions of the Ag 2 Te QDs and other two silver chalcogenide QDs (Ag 2 S and Ag 2 Se). Our findings indicate Ag 2 Te QDs have good biocompatibility in mice, which is a guarantee for their safe applications in the future.
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OPTICAL MATERIALS
·
2020
·
IF 2.779
Yingying Wei,Lin Chen,Junli Wang,Xuguang Liu,Yongzhen Yang,Shiping Yu
In order to avoid the interference of autofluorescence and improve tissue penetration capability of carbon quantum dots (CQDs) in biological imaging, the synthesis of long-wavelength emission CQDs was achieved in this work. Boron and nitrogen co-doped CQDs (BN-CQDs) were synthesized by one-step microwave assisted hydrothermal method with o -phenylenediamine as carbon and nitrogen source, boric acid as boron source. The BN-CQDs (QY = 13.79%) synthesized under the optimal conditions exhibit excitation independence, a large Stoke shift, satisfactory fluorescent stability, good biocompatibility and low toxicity, which can meet the basic requirements as cell imaging agent. Cell imaging measurements prove their increased intracellular accumulation with the extension of incubation time with HeLa cells, and clearly observable BN-CQDs imaging for cell morphology. Hence BN-CQDs can be used as a good cell imaging agent.
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