ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yaxin Xu,Jun Yan,Yu Zhu,Haoyu Chen,Cuiyan Wu,Xiaohua Zhu,Youyu Zhang,Haitao Li,Meiling Liu,Shouzhuo Yao
In this work, near-infrared fluorescent silver nanoclusters (Ag NCs) were prepared based on the in situ formed poly methacrylic acid (PMAA) as the template and stabilizer, which is synthesized by methacrylic acid (MAA) and hydroxyl radical (·OH) that is generated by the cascade nanoenzyme reaction of cupric oxide nanoparticles (CuO NPs). CuO NPs possess the intrinsic glutathione-like (GPx-like) and peroxidase-like (POD-like) activities, which can catalyze glutathione (GSH) and O2 to produce hydrogen peroxide (H2O2), and then transform into ·OH. The fluorescence intensity of Ag NCs decreases with the addition of GSH, because the −SH can easily anchor on the surface, resulting in the PMAA leaving the Ag NCs, and the coeffect of GSH and PMAA results in the aggregation to form larger Ag NPs. A good linear relationship between the fluorescence quenching rate and the GSH concentration was found in the range 0.01–40 μM with the detection limit 8.0 nM. The Ag NCs can be applied in the detection of GSH in the serum, as well as bioimaging of endogenous and exogenous GSH in cells with high sensitivity. Moreover, the normal and cancer cells can be distinguished through bioimaging because of the different GSH levels. The new method for the preparation of biocompatible nanoprobe based on the nanozyme tandem catalysis and the in situ formed template can avoid the direct usage of polymers or protein templates that hinder preparation and separation, providing a reliable approach for the synthesis, biosensing, and bioimaging of nanoclusters.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2022
·
IF 7.139
Huiren Liang,Zhengzhuan Xie,Ruolin Zhao,Xiaobin Wen,Feifan Hong,Weitao Shi,Haiqiang Chen,Haizhen Liu,Wenzheng Zhou,Jin Guo,Zhiqiang Lan
To improve the dehydrogenation/hydrogenation performance of magnesium hydride (MgH 2 ), a nickel-vanadium bimetallic oxide (NiV 2 O 6 ) was prepared by a simple hydrothermal method using ammonium metavanadate and nickel nitrate as raw materials. This oxide was used to improve the hydrogen storage performance of MgH 2 . NiV 2 O 6 reacted with Mg to form Mg 2 Ni and V 2 O 5 ; Mg 2 Ni and V 2 O 5 played an important role in improving the hydrogen storage properties of MgH 2 . The NiV 2 O 6 -doped MgH 2 had an excellent hydrogen absorption and desorption kinetics performance, and it could absorb 5.59 wt% of hydrogen within 50 min at 150 °C and release about 5.3 wt% of hydrogen within 12 min. The apparent activation energies for the dehydrogenation and hydrogenation of MgH 2 -NiV 2 O 6 were 92.9 kJ mol −1 and 24.9 kJ mol −1 , respectively. These were 21.7% and 66.3% lower than those of MgH 2, respectively. The mechanism analysis demonstrated that the improved kinetic properties of MgH 2 resulted from the heterogeneous catalysis of vanadium and nickel.
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JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 39
·期 15
·3054-3061
·
IF 2.741
Min Li Xu,Bao Qiong Li,Xue Wang,Jing Chen,Hong Lin Zhai
Although liquid chromatography with mass spectrometry in full scan mode can obtain all the signals simultaneously in a large range and low cost, it is rarely used in quantitative analysis due to several problems such as chromatographic drifts and peak overlap. In this paper, we propose a Tchebichef moment method for the simultaneous quantitative analysis of three active compounds in Qingrejiedu oral liquid based on three-dimensional spectra in full scan mode of liquid chromatography with mass spectrometry. After the Tchebichef moments were calculated directly from the spectra, the quantitative linear models for three active compounds were established by stepwise regression. All the correlation coefficients were more than 0.9978. The limits of detection and limits of quantitation were less than 0.11 and 0.49 μg/mL, respectively. The intra- and interday precisions were less than 6.54 and 9.47%, while the recovery ranged from 102.56 to 112.15%. Owing to the advantages of multi-resolution and inherent invariance properties, Tchebichef moments could provide favorable results even in the situation of peaks shifting and overlapping, unknown interferences and noise signals, so it could be applied to the analysis of three-dimensional spectra in full scan mode of liquid chromatography with mass spectrometry.
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EBioMedicine
·
2019
·
IF 6.68
Xiaoyu Liao,Lingyu Song,Benhua Zeng,Bingyao Liu,Yuyang Qiu,Hua Qu,Yi Zheng,Min Long,Houdi Zhou,Yuan Wang,Yingxuan Du,Jing Xu,Rufei Shen,Qiang Tong,Leiqin Cai,Xing Li,Shaodong Guo,Gangyi Yang,Zhiming Zhu,Xiaoyun Pu,Hong Wei,Hongting Zheng
Background Increasing evidence indicates that the gut microbiota contributes to the occurrence and development of metabolic diseases. However, little is known about the effects of commonly used antidiabetic agents on the gut microbiota. In this study, we investigated the roles of dipeptidyl peptidase-4 inhibitors (DPP-4i) and α-glucosidase inhibitor in modulating the gut microbiota. Methods 16S-rDNA sequencing was performed to analyse the effects of DPP-4i and acarbose on the gut microbiota in mice fed a high-fat diet (HFD). Fecal microbiota transplantation (FMT) from type 2 diabetes patients to germ-free mice was performed to investigate the contribution of the altered microbiome to antidiabetic effects of the drugs. Fecal metabolomics was also analysed by untargeted and targeted GC–MS systems. Findings Although DPP-4i and α-glucosidase inhibitor both altered the gut microbial composition, only the microbiome modulation of DPP-4i contributed to its hypoglycemic effect. Specifically, the changes of 68.6% genera induced by HFD were rescued by DPP-4i. FMT showed that the DPP-4i-altered microbiome improved glucose tolerance in colonized mice, while acarbose did not. Moreover, DPP-4i increased the abundance of Bacteroidetes, and also promoted a functional shift in the gut microbiome, especially increasing the production of succinate. Interpretation Our findings demonstrate an important effect of DPP-4i on the gut microbiota, revealing a new hypoglycemic mechanism and an additional benefit of it. Furthermore, modulating the microbial composition, and the functional shift arising from changes in the microbiome, might be a potential strategy for improving glucose homeostasis. Fund This work was supported by grants from the National Natural Science Foundation of China (No. 81700757, No. 81471039, No. 81700714 and No. 81770434), the National Key R&D Program of China (No. 2017YFC1309602, No. 2016YFC1101100, No. 2017YFD0500503 and No. 2017YFD0501001), and the Natural Science Foundation of Chongqing (No. cstc2014jcyjjq10006, No. cstc2016jcyjA0093 and No. cstc2016jcyjA0518).
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Chen Yu,Xinlai Dou,Linghan Meng,Xiao Feng,Chengcheng Gao,Fenglian Chen,Xiaozhi Tang
Hydrogels, owing to their unique porous structures, hydrophilic properties, and biocompatibility, are being developed as scaffolds for bone grafts. However, the use of toxic initiators or cross-linking agents is a drawback. To overcome this, we developed Laponite®/cross-linked starch/polyvinyl alcohol (PVA) hydrogels prepared by one-step solution mixing. The structure, rheological properties, and biocompatibility of the hydrogels were investigated. Zeta potential , Fourier transform infrared, and X-ray diffraction analyses showed that hydrogen bonding and electrostatic interactions jointly maintained the structure of the cross-linked hydrogel systems. At a Laponite® concentration of 10 %, the hydrogel with a starch/PVA ratio of 2:2 exhibited a uniform porous structure, the highest storage modulus (G'), and the lowest degradation rate. At a starch/PVA ratio of 2:2, the G' increased; however, the degradation rate decreased with the increase in Laponite® content from 5 % to 20 %. These results indicate that the mechanical strength and degradation rate of the hydrogels could be adjusted by altering the starch/PVA ratio and the amount of Laponite®. In vitro cytotoxicity experiments showed that the Laponite®/starch/PVA (LSP) hydrogels were non-toxic to MC3T3-E1 cells. The starch/PVA ratio had no obvious effect on the proliferation of MC3T3-E1 cells, but an increase in Laponite® content significantly promoted cell proliferation . In summary, the results suggest that these LSP hydrogels have great potential for applications in bone tissue engineering.
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Frontiers in Plant Science
·
2023
·
IF 5.6
Xia Wu,Feng Wei,Furong Ding,Nian Yang,Jingming Niu,Yuanquan Ran,Minyi Tian
Alpinia coriandriodora , also known as sweet ginger, is a medicinal and edible plant. A. coriandriodora rhizome is popularly utilized in traditional Chinese medicine and as flavouring spices, but there are few reports on its constituents and bioactivities. This study analyzed the phytochemical components of A. coriandriodora rhizome by GC-MS and UHPLC-Q-Orbitrap-MS and evaluated its antioxidant, antimicrobial, and anti-enzymatic properties. According to the GC-FID/MS data, its rhizome essential oil (EO) consisted mainly of ( E )-2-decenal (53.8%), ( E )-2-decenyl acetate (24.4%), ( Z )-3-dodecenyl acetate (3.5%), and ( E )-2-octenal (3.5%). Its water extract (WE) and 70% ethanol extract (EE) showed high total phenolic content (TPC, 52.99–60.49 mg GAEs/g extract) and total flavonoid content (TFC, 260.69–286.42 mg REs/g extract). In addition, the phytochemicals of WE and EE were further characterized using UHPLC-Q-Orbitrap-MS, and a total of sixty-three compounds were identified, including fourteen phenolic components and twenty-three flavonoid compounds. In the antioxidant assay, WE and EE revealed a potent scavenging effect on DPPH (IC 50 : 6.59 ± 0.88 mg/mL and 17.70 ± 1.15 mg/mL, respectively), surpassing the BHT (IC 50 : 21.83 ± 0.89 mg/mL). For the antimicrobial activities, EO displayed excellent antibacterial capabilities against Proteus vulgaris , Enterococcus faecalis , Bacillus subtilis , Escherichia coli , and Staphylococcus aureus with DIZ (12.60–22.17 mm), MIC (0.78–1.56 mg/mL), and MBC (3.13 mg/mL) and significantly inhibited Aspergillus flavus growth (MIC = 0.313 mg/mL, MFC = 0.625 mg/mL, respectively). In addition to weak tyrosinase and cholinesterase inhibition, EE and WE had a prominent inhibitory effect against α-glucosidase (IC 50 : 0.013 ± 0.001 mg/mL and 0.017 ± 0.002 mg/mL), which was significantly higher than acarbose (IC 50 : 0.22 ± 0.01 mg/mL). Hence, the rhizome of A. coriandriodora has excellent potential for utilization in the pharmaceutical and food fields as a source of bioactive substances.
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Journal of Materials Chemistry A
·
2020
·Vol. 8
·期 40
·21327-21338
·
IF 11.301
Min Bao,Xinghua Chen,Shiyu Hu,Linqun Zhang,Ying Li,Carlo Carraro,Roya Maboudian,Wei Wei,Yuanjian Zhang,Songqin Liu
In this work, a dual catalyst composed of atomically ordered intermetallic PdZn and Co nanoparticles was synthesized and chemically bonded to nitrogen-doped carbon by simultaneously modulating the lattice spacing of the Pd nanoparticle guest and the metal centers of the zeolite imidazolate framework (ZIF) host through a one-step heating procedure. Results reveal that the ability of the PdZn alloy to catalyze the oxygen reduction reaction can be regulated by adjusting the lattice spacing of Pd–Pd through the formation of the ordered PdZn, resulting from Zn2+ in the ZIF-8 host lattice diffusing into the Pd phase. Meanwhile, the secondary active sites (Co nanoparticles) and N-doped carbon (particularly N-doped carbon nanotubes) carrier are obtained by doping Co2+ into the ZIF-8 host lattice, which can further improve the catalytic activity. The characterization results show the existence of Pd–N and Co–N bonds between the dual catalyst and the carrier, which can be regulated by controlling the synthesis conditions, such as the atomic ratios of the Pd guest to Zn in the ZIF-8 host, pyrolysis temperatures, and doping Co2+ into the ZIF-8 host lattice. The dual catalyst bonded to N-doped carbon exhibits high ORR catalytic activity with a desirable onset potential (0.916 V), and diffusion-limiting current density (∼5.70 mA cm−2), superior to other reported Pd-based catalysts and comparable to commercial Pt/C. Moreover, the chemical bonds between the dual catalyst and the carrier result in the dual catalyst with a small size of 5.9 nm and prolonged duration of electrochemical activity (no obvious change of the half peak potential after 10 000 cyclic voltammetry cycles and 90.6% current retention after a 4 h chronoamperometric test). Thus, the highly dispersed ordered-PdZn/Co on N-doped carbon is a promising substitute to commercial Pt/C for alkaline fuel cells.
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CHEMISTRY-A EUROPEAN JOURNAL
·
2022
·Vol. 28
·期 19
·e202104623
·
IF 5.02
Jin Yuan,Jinqiu Guo,Zhiwei He,Leisheng Che,Shanshan Chen,Hongbo Zhang
Graphical The photo-energy and thermal-energy in a synergetic way promote catalytic reaction together. In this article, the C−H bond rupture within HCOOH* was determined to be the rate-determining step with adsorbed CO* as the most abundant surface intermediates (MASIs) under both thermal and photo-thermal reaction conditions confirmed by kinetic isotopic effects and in situ FTIR characterizations. Clear evidence of kinetically relevant consistency was determined under both thermal and photo-thermal HCOOH decomposition reactions over Pd/LaCrO 3 /C 3 N 4 composite. Photo-thermal catalysis has been an attractive alternative strategy to promote chemical reactions for years, however, how light cooperates with thermal energy is still unclear. We meet this demand by exploring reaction mechanism via pressure dependency studies as well as H/D exchange experiments with HCOOH decomposition as a probe over a palladium nanoparticle (Pd n ) and isolated Pd (Pd 1 ) decorated LaCrO 3 /C 3 N 4 composite catalyst, in which the H 2 formation rate shows a first-order dependence on HCOOH and inverse first-order dependence on CO partial pressures no matter the reaction was driven by thermal or photo-thermal energy. Additionally, negligible kinetic isotopic effects (KIEs: k H / k D ) were determined under both dark and light conditions at 1.04 and 1.18 when the HCOOH was replaced by HCOOD. Besides, when the reactant HCOOH was further replaced by DCOOD, the KIE values of 1.55 (dark) and 1.92 (light) were obtained, which indicates that the HCOOH decomposition follows kinetically relevant (KR) of C−H bond rupture within HCOOH molecule under both thermal and photo-thermal reaction conditions and the catalytic surface was found to be densely covered by CO based on the pressure dependency studies as well as the in situ Fourier transform infrared spectroscopy (FTIR) analysis. Clearly, the HCOOH decomposition driven by thermal and photo-thermal energy follows the same reaction mechanism. Nevertheless, light induced hot electrons and the derived thermal effect do cause the enhancement of the reaction activity in some circumstances compared with bare thermal catalysis, which clarifies the confusion on cooperation mechanism of photo and thermal energies from the kinetic perspective. Hot electrons induced by photo-illumination was confirmed by in situ FTIR CO chemisorption with ∼10 cm −1 redshift identified of the CO feature once light was introduced. Meanwhile, the photo thermal reaction system suffers from severe electron-hole re-combination at high reaction temperatures and make the thermal effect of photo irradiation dominant with respect to the effect at low reaction temperatures. This research provides insight to the mechanism on how photo-thermal reaction works and draws attention to the photo-thermal reaction process in boosting catalytic activity.
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Materials Today Communications
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2023
·
IF 3.8
Yan Lin,Yanjing Ou,Minrui Xu,Jiang Chen
Surface modification can enhance the biological properties of polycaprolactone (PCL) scaffolds. In this study, bionic hydrolysis and biomimetic polydopamine (PDA) coating were investigated, individually and in combination, to enhance the bioactivity of inner cube 3D-printed PCL scaffolds. The modified scaffolds were evaluated for their bioactivity, and the underlying mechanism was studied based on their physiochemical properties. The modifications significantly improved the surface roughness , hydrophilicity, surface adhesion ability, and biological properties of the scaffolds. The hydrolyzed PCL scaffolds exhibited micro-cracks and pits on their surface, with carboxyl and hydroxyl groups present. PDA, in the form of micro-particles with phenolic hydroxyl and amino groups, was coated onto the PDA-coated scaffolds. The combination-treated scaffolds had shallower pits, smaller particles, and lower levels of phenolic hydroxyl and amino groups. PDA coating demonstrated the best properties in promoting the adhesion, proliferation, and osteogenesis of rat bone marrow mesenchymal stem cells. Although the combination-treated scaffolds showed better cell proliferation than the hydrolyzed scaffolds, they did not exhibit a synergistic effect on osteogenic induction, instead weakening the osteogenic induction ability of the PDA-coating and hydrolysis treatments. Therefore, the material’s characteristics and the substantial variation of different chemical reactions must be considered during biological material modification.
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Journal of Functional Biomaterials
·
2023
·Vol. 14
·期 1
·43
·
IF 4.8
Xiaoyu Wang,Yuyang Zhou,Melissa Johnson,Cameron Milne,Sigen A,Yening Li,Wenxin Wang,Nan Zhang,Qian Xu
To explore the potential applicability of chitosan (CTS), we prepared aldehyde chitosan (CTS-CHO) with chitosan and sodium periodate via oxidation reaction and then a chitosan-based hydrophilic and antibacterial coating on the surface of poly (lactic acid) (PLA) film was developed and characterized. The oxidation degree was determined by Elemental analyser to be 12.53%, and a Fourier transform infrared spectroscopy was used to characterize the structure of CTS-CHO. It was evident that CTS-CHO is a biocompatible coating biomaterial with more than 80% cell viability obtained through the Live/Dead staining assay and the alamarBlue assay. The hydrophilic and antibacterial CTS-CHO coating on the PLA surface was prepared by ultrasonic atomization assisted LbL assembly technique due to Schiff’s base reaction within and between layers. The CTS-CHO coating had better hydrophilicity and transparency, a more definite industrialization potential, and higher antibacterial activity at experimental concentrations than the CTS coating. All of the results demonstrated that the ultrasonic atomization-assisted LbL assembly CTS-CHO coating is a promising alternative for improving hydrophilicity and antibacterial activity on the PLA surface. The functional groups of CTS-CHO could react with active components with amino groups via dynamic Schiff’s base reaction and provide the opportunity to create a drug releasing surface for biomedical applications.Keywords:chitosan;PLA;hydrophilic;antibacterial;coating
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Pharmaceuticals
·
2023
·Vol. 16
·期 5
·767
·
IF 4.6
Jie Chen,Xiang Zhou,Lifeng Fu,Haiyu Xu
Drugs that cure COVID-19 have been marketed; however, this disease continues to ravage the world without becoming extinct, and thus, drug discoveries are still relevant. Since Mprohas known advantages as a drug target, such as the conserved nature of the active site and the absence of homologous proteins in the body, it receives the attention of many researchers. Meanwhile, the role of traditional Chinese medicine (TCM) in the control of epidemics in China has also led to a focus on natural products, with the hope of finding some promising lead molecules through screening. In this study, we selected a commercial library of 2526 natural products from plants, animals and microorganisms with known biological activity for drug discovery, which had previously been reported for compound screening of the SARS CoV-2 S protein, but had not been tested on Mpro. This library contains compounds from a variety of Chinese herbs, includingLonicerae Japonicae Flos,Forsythiae FructusandScutellariae Radix, which are derived from traditional Chinese medicine prescriptions that have been shown to be effective against COVID-19. We used the conventional FRET method for the initial screening. After two rounds of selection, the remaining 86 compounds were divided into flavonoids, lipids, phenylpropanoids, phenols, quinones, alkaloids, terpenoids and steroids according to the skeleton structures, with inhibition rates greater than 70%. The top compounds in each group were selected to test the effective concentration ranges; the IC50values were as follows: (−)–gallocatechin gallate (1.522 ± 0.126 μM), ginkgolic acid C15:1 (9.352 ± 0.531 μM), hematoxylin (1.025 ± 0.042 μM), fraxetin (2.486 ± 0.178 μM), wedelolactone (1.003 ± 0.238 μM), hydroxytyrosol acetate (3.850 ± 0.576 μM), vanitiolide (2.837 ± 0.225 μM), β,β–dimethylacrylalkannin (2.731 ± 0.308 μM), melanin (7.373 ± 0.368 μM) and cholesteryl sodium sulfate (2.741 ± 0.234μM). In the next step, we employed two biophysical techniques, SPR and nanoDSF, to obtain KD/Kobsvalues: hematoxylin (0.7 μM), (−)–gallocatechin gallate (126 μM), ginkgolic acid C15:1 (227 μM), wedelolactone (0.9770 μM), β,β–dimethylacrylalkannin (1.9004 μM,), cholesteryl sodium sulfate (7.5950 μM) and melanin (11.5667 μM), which allowed better assessments of the binding levels. Here, seven compounds were the winners. Then, molecular docking experiments were specially performed by AutoDock Vina to analyze the mode of interactions within Mproand ligands. We finally formulated the present in silico study to predict pharmacokinetic parameters as well as drug-like properties, which is presumably the step that tells humans whether the compounds are drug-like or not. Moreover, hematoxylin, melanin, wedelolactone, β,β–dimethylacrylalkannin and cholesteryl sodium sulfate are in full compliance with the “Lipinski” principle and possess reasonable ADME/T properties, they have a greater potential of being lead compounds. The proposed five compounds are also the first to be found to have potential inhibitory effects on SARS CoV-2 Mpro. We hope that the results in this manuscript may serve as benchmarks for the above potentials.Keywords:SARS CoV-2 Mpro;natural products;high-throughput screening;lead compounds;in silico study
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NANOTECHNOLOGY
·
2021
·Vol. 32
·期 28
·285101
·
IF 3.874
Xiaoyan Wu,Yingying Hua,Tiantian Wei,Chenjun Ma,Zhongjie Wang,Liefeng Zhang,Jing Wang
In this study, a cyclodextrin derivative (R6RGD-CMβCD) nanoparticle with tumor targeting and cell penetration ability was successfully synthesized and loaded with tyroserleutide (YSL) to obtain YSL-loaded nanoparticles (YSL/R6RGD-CMβCD NPs). The characterization of these NPs revealed a smooth surfaces and an average diameter of approximately 170 nm. YSL/R6RGD-CMβCD NPs increased the NP uptake in Caco-2 cells. As regard the mechanism of action, the cell uptake was related to endocytosis mediated by reticulin and megacytosis. In addition, YSL/R6RGD-CMβCD NPs induced significantly higher cytotoxicity on tumor cells and better tumor targeting compared with the effect of CMβCD NPs. Most importantly, the good anti-cancer effect of YSL/R6RGD-CMβCD NPs might be due to the interference with the function of mitochondria. On the other hand, YSL/R6RGD-CMβCD NPs were not toxic for normal cells. Taken together, our results indicated that R6RGD-CMβCD could be considered as a nanopharmaceutical material with good tumor targeting abilities, and their combination with YSL could represent an effective anti-cancer system.
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