JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 12
·8398-8409
·
IF 3.2
Junguo Wang,Hongyue Wang,Keshuang Guo,Xindi Zhang,Xiangrong Zhang
The purpose of this study was to prepare and evaluate the formulation of nanoemulsions (NEs) to encapsulate phloretin (PT) to improve its stability, antioxidant, and tyrosinase inhibitory competence. The aim of this study was to improve the stability, antioxidant, and tyrosinase inhibitory effects of PT via NEs. The formulations were prepared using low energy emulsification method for PT-VE-NEs, α -tocopherol (Vitamin E) and medium chain triglycerides (MCT) were used as the oil phase, and Tween 60 was used as the emulsifier and PEG-400 as the co-emulsifier. The droplet size and zeta potential of oil-in-water NEs were evaluated using dynamic light scattering. The PT-VE-NEs were also characterized by transmission electron microscopy and Fourier transform infrared spectroscopy. The mean droplet diameter was 14.85 ± 0.14 nm, with a zeta potential of −2.47 ± 0.51 mV. Fourier transform infrared spectroscopy revealed the formation of molecular interactions in the NEs formulations. PT-VE-NEs size was maintained the same during the in vitro digestion study. The particle size of PT-VE-NE remained stable during in vitro digestion. The addition of VE significantly improved the antioxidant, tyrosinase inhibitory effects, as well as thelion and physical stability of PT-VE-NE. The results revealed that NEs is a promising strategy to improve the functionality and stability of PT and VE. PT-VE-NEs will be applied for the preservation of fruits.
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高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2024
·
IF 4.5
Mengcheng Jin,Tangbin Zou,Hairong Huang,Ming Chen,Haoqi Zou,Baoyan Chen,Chengze Lai,Huawen Li,Peiwen Zhang
Scope Bile acids play a crucial role in lipid absorption and the regulation of lipid, glucose, and energy homeostasis. Coenzyme Q10 (CoQ10), a lipophilic antioxidant, has been recognized for its positive effects on obesity and related glycolipid metabolic disorders. However, the relationship between CoQ10 and bile acids has not yet been evaluated. Methods and results This study assesses the impact of CoQ10 treatment on bile acid metabolism in mice on a high-fat diet using Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry. CoQ10 reverses the reduction in serum and colonic total bile acid levels and alters the bile acid profile in mice that are caused by a high-fat diet. Seventeen potential targets of CoQ10 in bile acid metabolism are identified by network pharmacology, with six being central to the mechanism. Molecular docking shows a high binding affinity of CoQ10 to five of these key targets. Further analyses indicate that farnesoid X (FXR) receptor and Takeda G-protein coupled receptor 5 (TGR5) may be crucial targets for CoQ10 to regulate bile acid metabolism and exert beneficial effects. Conclusion This study sheds light on the impact of CoQ10 in bile acids metabolism and offers a new perspective on the application of CoQ10 in metabolic health.
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DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2024
·
IF 2.4
Xingbo Wang,Hong Huang,Wenxiu Xu,Yanling Gong,Songbo Shi,Xu Wan,Li Pengbiao
Introduction Triple negative breast cancer (TNBC) is characterized with higher malignancy and mortality and is prone to distant metastasis, among which bone is the most common site. It’s urgent to explore new strategies for treatment of TNBC and its bone metastases.Methods A tumor environment responsive vector, poly-(dimethylaminoethyl methacrylate)-SS-poly(ethylene glycol)-SS-poly-(dimethylaminoethyl methacrylate) (PDMAEMA-SS-PEG-SS-PDMAEMA), was constructed to co-delivery transforming growth factor-β1 (TGF-β1) siRNA and forkhead box M1 (FOXM1) siRNA in MDA-MB-231 cells. The preparation, characterization, in vitro release, stability, and transfection efficiency of nanoparticles were measured. Cell viability, migration and invasion of MDA-MB-231 cells were determined. Cell chemotactic migration and cell heterogeneity adhesion of MDA-MB-231 cells to the human osteoblast-like cell line MG-63 were determined.Results PDMAEMA-SS-PEG-SS-PDMAEMA self assembled with siRNA at N/P of 15:1 into nanoparticles with particle size of 122 nm. In vitro release exhibited redox and pH sensitivity, and the nanoparticles protected siRNA from degradation by RNase and serum protein, remaining stable at 4 °C with similar transfection efficiency with lipo2000. Nanoparticles co-loaded with TGF-β1 siRNA and FOXM1 siRNA inhibited the cell viability, migration and invasion of MDA-MB-231 cells, as well as chemotactic migration and heterogeneous adhesion of MDA-MB-231 cells to MG-63 cells, showing a synergetic effect. After gene silencing on TGF-β1 and FOXM1, the epithelial to mesenchymal transition (EMT) related molecules vimentin mRNA expression decreased while E-cadherin increased.Conclusion PDMAEMA-SS-PEG-SS-PDMAEMA was suitable for TGF-β1 siRNA and FOXM1 siRNA delivery, exhibiting synergetic inhibition effect on TNBC and its bone metastases, which might be related to its synergetic inhibition on EMT.
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Materials Today Bio
·
2024
·
IF 8.7
Tianming Lu,Zixian Liu,Ruoning Qian,Yitian Zhou,Jun Li,Qiang Zhang,Hao Yang,Wenli Lu,Yanlin Xin,Zejuan Xie,Lesan Yan,Shanshan Wang,Ruogu Qi,Zhengguang Zhang
Sorafenib (SF), a multi-targeted tyrosine kinase inhibitor, serves as a primary therapeutic modality for advanced liver cancer. Nonetheless, its clinical efficacy is hindered by various obstacles, such as limited bioavailability and inadequate accumulation. This study introduces a novel biomimetic mineralization enzyme, known as BSA@Pt/Ce6/SF@M (PCFM). The PCFM incorporates platinum (Pt) as a catalytic agent, SF as a molecular-targeted therapeutic agent, and Ce6 as a photosensitizer within liver cancer cell membranes. This strategy enables the combination of various anti-tumor treatments, such as photodynamic therapy (PDT) and autophagy induction, leading to increased bioavailability of SF and achieving a multidimensional synergistic anticancer effect. The PDT effect produced by Ce6 in PCFM greatly enhances SF-induced autophagy, effectively promoting autophagic cell death. Furthermore, Pt dissociates from the biomineralization process, acquiring peroxidase properties through chemokinetic reactions. This facilitates the catalysis of significant oxygen generation, addressing the challenge of hypoxia in the tumor microenvironment and improving the efficacy of PDT. Moreover, the SF enhances therapeutic efficacy by inducing autophagy in response to energy deprivation, as indicated by the reduced levels of HIF-1α, p62, along with increased levels of ROS and LC3-Ⅱ. This biomineralization-based nanoenzyme exhibits strong anti-tumor characteristics, offering a novel strategy for overcoming challenges in liver cancer treatment.
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LIVER INTERNATIONAL
·
2024
·
IF 6
Li-Shuang Hou,Xiao-Pei Zhai,Yao-Wen Zhang,Jie-Hua Xing,Chen Li,Si-Yuan Zhou,Xiao-Hong Zhu,Bang-Le Zhang
Background and Purpose Liver fibrosis is a wound-healing reaction which is the main cause of chronic liver diseases worldwide. The activated hepatic stellate cell (aHSC) is the main driving factor in the development of liver fibrosis. Inhibiting autophagy of aHSC can prevent the progression of liver fibrosis, but inhibiting autophagy of other liver cells has opposite effects. Hence, targeted inhibition of autophagy in aHSC is quite necessary for the treatment of liver fibrosis, which prompts us to explore the targeted delivery system of small molecule autophagy inhibitor hydroxychloroquine (HCQ) that can target aHSC and alleviate the liver fibrosis. Methods The delivery system of HCQ@retinol-liposome nanoparticles (HCQ@ROL-LNPs) targeting aHSC was constructed by the film dispersion and pH-gradient method. TGF-β-induced HSC activation and thioacetamide (TAA)-induced liver fibrosis mice model were established, and the targeting ability and therapeutic effect of HCQ@ROL-LNPs in liver fibrosis were studied subsequently in vitro and in vivo. Results HCQ@ROL-LNPs have good homogeneity and stability. They inhibited the autophagy of aHSC selectively by HCQ and reduced the deposition of extracellular matrix (ECM) and the damage to other liver cells. Compared with the free HCQ and HCQ@LNPs, HCQ@ROL-LNPs had good targeting ability, showing enhanced therapeutic effect and low toxicity to other organs. Conclusion Construction of HCQ@ROL-LNPs delivery system lays a theoretical and experimental foundation for the treatment of liver fibrosis and promotes the development of clinical therapeutic drugs for liver diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Safdar Ali Amur,Quratulain Khuhro,Najaf Ali Soomro,Bharat Prasad Sharma,Ahmed Nadeem,Muhammad Tariq,Hao Liang
ZIF-8 has strong potential for biomedical applications due to its excellent biocompatibility. In this study, 89.67 % of natural drug magnolol (Mag) was loaded in ZIF-8 to form Mag@ZIF-8, which was subsequently modified with chitosan (CS) to obtain Mag@ZIF-8/CS. The prepared materials were characterized using the UV–Vis spectroscopy, FTIR, XRD, DLS, SEM and TEM techniques. Thermal stability of materials was analyzed using Thermos Gravimetric Analysis system. PDI and Z -average hydrodynamic sizes of Mag@ZIF-8/CS and Mag@ZIF-8 were increased compared to ZIF-8. Similarly, Mag@ZIF-8 revealed higher zeta potential (+17.5 ± 2.3 mV) than ZIF-8 (+9.8 ± 0.5 mV), but Mag@ZIF-8/CS exposed 3.2 ± 0.9 mV, in water; however, at pH 4 it was changed to +5 mV. Pure ZIF-8 and Mag@ZIF-8 exhibited a hexagonal morphology, while Mag@ZIF-8/CS had a rotund, flat-like structure, with mean particle size of 101 nm, 123 nm and 343 nm, respectively. Density Functional Theory (DFT) analysis offered a deeper insight into the electronic properties and binding interactions of the composites. Under acidic conditions, drug release from Mag@ZIF-8 was higher (86.4 %) and faster than from Mag@ZIF-8/CS (74 %), when tested at physiological pH 7.4 and 37 °C. This, suggests that CS modification of Mag@ZIF-8 is crucial for achieving prolonged and sustained drug release. Mag@ZIF-8 and Mag@ZIF-8/CS depicted a two-fold increase in antibacterial activity against E. coli and S. aureus , demonstrating a synergistic effect. The MIC value for E. coli and S. aureus were, 35 μg/mL and 20 μg/mL, respectively, confirming the enhanced antibacterial efficacy of the composites. Molecular docking analysis provided deeper insights into the binding interactions between the materials and the E. coli protein 5AZC.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Han Zhang,Yao Lu,Rui Zhang,Zhe Tang,Shiu Hei Lam,Jinjin Zhu,Runfang Fu,Yeting Hu,Muhammad Zubair Iqbal,Xiangdong Kong,Jianfang Wang
Plasmonic nanomaterials with multiple components and designed structures show diverse properties and functions owing to the synergistic effects of the different components. Engineering the compositional and structural complexity of plasmonic nanomaterials is a powerful technique for expanding their range of applications. A facile approach to an unprecedented dart-like type of nanostructure with AgPd bimetal selectively deposited at one end of Au nanobipyramids, forming AgPd nanodarts, is reported here. The synthetic process involves both galvanic replacement and Ostwald ripening. The morphological evolution of the AgPd nanodarts is thoroughly investigated and the growth mechanism is put forward. The as-obtained AgPd nanodarts exhibit an outstanding photothermal conversion efficiency and peroxidase activity, which make them powerful for photothermally–catalytically combined tumor organoid ablation. In addition, two types of AgPd nanodart-derived single-end nanostructures, AgPd@ZIF-8, and AgPd@TiO 2 nanodarts, are prepared for the first time. The ZIF-8 and TiO 2 shell are preferentially coated at the AgPd end of the AgPd nanodarts, respectively. Both AgPd@ZIF-8 and AgPd@TiO 2 nanodarts show superior antimicrobial activities. The protocol and concept of modulating the structure of the nanodarts in this work can be extended to the development of a new class of plasmonic nanostructures with multifunctionalities.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2025
·Vol. 27
·期 1
·1-15
·
IF 2.6
Wu Gui-Lin,Deng Tian-Song,Wang Kun-Peng,Zhang Er-Ji,Liu Li-Yong,Cheng Yu-Chun,Gao Jia-Fei,Liu Jie
Gold nanorods are widely used in surface-enhanced Raman scattering (SERS) applications due to their strong localized surface plasmon resonance (LSPR). The self-assembly of gold nanorods further expands their applications and introduces new ensemble properties. In this paper, two types of gold nanorods with similar lengths but significantly different diameters were used for making superparticles. The gold nanorods were first surface-modified with thiol-terminated polystyrene (PS-SH), and then assembled into superparticles through emulsion volatilization. To test the SERS performances of the gold nanorod superparticles, Nile red was used as a dye molecule to test the localization of the SERS performance of the single superparticles. It was found that as the size of the superparticles increased, the SERS performances also improved, with the final Raman signal intensity reaching up to 2 × 10 6 . When the size of the superparticles was the same, the SERS performance was stronger for superparticles composed of gold nanorods with larger diameters. Additionally, a structure similar to the superparticles was designed for FDTD simulations, and the simulated results were highly consistent with the experimental results, further supporting our conclusions.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2024
·
IF 4.8
Yuxin Li,Juanping Zhou,Hu Wen,Haitao Yin,Shunyu Han,Nanzhe Jiang
Clay is a rich aluminosilicate resource with unique advantages as a raw material for zeolites production. However, synthesizing nanosized single-crystalline zeolites through a simple approach using clay remains a significant challenge. In this paper, acid-washed montmorillonite (MSR) was used to prepare nano-H-ZSM-5 single crystals with high crystallinity, uniform grain size and permeable straight pores under the induction of a small amount of organic template tetrapropylammonium hydroxide (TPAOH). Through temperature-staged treatment, the nucleation and growth process of zeolite were decoupled, ultimately achieving precise control over the size of the nano-single crystals. Mechanistic study showed that the low-temperature aging process produces smaller structural units which probably derived from the geopolymer topology of the clay, providing more active sites for zeolite nucleation, leading to the rapid crystallization during the high-temperature process. The porous structure promotes material diffusion, inhibits aromatic cycling and reduces carbon formation in the pores. The large specific surface area and nanoscale multilayer pore structure delay carbon accumulation and increase carbon holding capacity. These properties make nano-ZSM-5 single crystals an excellent choice for high olefin selectivity and long catalytic life in the methanol-to-olefin reaction. This synthetic route provides a general strategy for preparing nanosized zeolite single crystals from clay.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·Vol. 142
·期 20
·e56887
·
IF 2.8
Lei Zhang,Yilin Wang,Weixia Zhou,Dan He,Yiming Xiao,Haoran Chen,Na Tang
Conventional gas separation membranes are mostly aimed at improving the permeability and selectivity of membranes. However, the actual application environment is complex and changeable, and the failure of the material is a common problem in the use process. Fluorinated polyurethane acrylate was synthesized via the fluorinated unit alcohol end-capping approach to enhance the antioxidant capacity of gas separation membranes. The fluorine element is used as an important part of the membrane structure, and then the antioxidant capacity of the membrane is enhanced. Specifically, hexamethylene diisocyanate isocyanurate (HDI) trimer and 1H, 1H, 2H, 2H-Tridecafluorooctanol (TFOA) were precisely metered and underwent an addition reaction. Subsequently, two molecules of the resulting addition products were connected to a molecule of 1,6-hexanediol (HDO). This was followed by a nucleophilic addition reaction between hydroxyethyl methacrylate and the fluorine-rich molecular formula, yielding fluorinated polyurethane acrylate (FPUA). To fabricate the gas separation membrane, photoinitiators were incorporated into the FPUA, followed by curing under UV irradiation. Under optimized conditions, the membrane exhibited exceptional performance in CO 2 /SF 6 separation, achieving a gas selectivity of 126.11. The presence of abundant carbon, fluorine, and oxygen atoms in the separation layer significantly bolstered its antioxidant properties. After immersion in a 1 g/L NaClO solution for 7 days, the membrane exhibited minimal corrosion loss of only 1.5% ± 1.3%, and the water contact angle decreased by just 1.8°. This robust membrane material offers promise for the separation of mixed gases containing oxidizing components.
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JOURNAL OF CHEMICAL RESEARCH
·
2024
·
IF 1
Shunze Gong,Chixiang Yuan,Hang Hu,Defeng Xu
In this work, 20 curcumin derivatives containing halogen atoms or nitrogen atoms were synthesized for suppression of castration-resistant prostate cancer. These curcumin derivatives were prepared by aldol condensation between the substituted benzaldehydes and acetylacetone or ethyl 3-acetyl-4-oxopentanoate. All synthesized compounds were characterized by 1H nuclear magnetic resonance, 13C nuclear magnetic resonance, high resolution mass, ultraviolet–visible spectroscopy, fluorescence spectroscopy, and high-performance liquid chromatography. The in vitro cytotoxicity of the prepared compounds against 22Rv1 cells was evaluated by the standard MTT assay. The compounds with good inhibitory activity against 22Rv1 cells were further tested for androgen receptor suppression effect. The results show that all compounds were successfully prepared and the purities are all over 90%. Among all synthesized compounds, compound p and s exhibit better inhibitory activity against 22Rv1 cells as compared to dimethylcurcumin, compound q and r display similar inhibitory activity against 22Rv1 cells as compared to dimethylcurcumin. Compounds p-s also demonstrate enhanced androgen receptor suppression effect against 22Rv1 cells as compared to dimethylcurcumin. This work indicates that compounds p-s show promising inhibitory activity against castration-resistant prostate cancer cells by suppression of androgen receptor.
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DYES AND PIGMENTS
·
2025
·
IF 4.2
Tingye Gu,Qing Guo,Dongming Qi,Pu Gao,Weiguo Chen,Zhihua Cui,Sateesh Bandaru
N -hydroxysuccinimide esters (NHS esters) are widely recognized as amine-reactive compounds due to their mild reaction conditions and high efficiency. In this study, NHS ester group was introduced into the dye chromophores to design and synthesize carboxylic acid succinimidyl ester dyes ( CSED s) in red, yellow and blue colors. The CSED s were characterized using high-resolution mass spectrometry, proton nuclear magnetic resonance, and infrared spectroscopy. When the NHS ester group was introduced, UV–vis absorption spectroscopy only slightly affected the molar extinction coefficient and maximal absorption wavelength. Hydrolysis studies demonstrated that CSED s hydrolyzed slowly in acidic environments but quickly in weakly alkaline ones, with near-complete hydrolysis taking place at pH 8.5 after half an hour. It was discovered that CSED s could successfully dye silk fibroin at room temperature in weakly alkaline conditions, producing a high color depth and more than 95 % color fixing. The binding of CSED s to silk fibroin was confirmed through covalent bonds using DMF stripping method and dialysis method. Furthermore, the color fastness of dyed silk fibroin to washing and rubbing was rated above grade 4–5. Compared to commercial dyes, the milder dyeing conditions of CSED s can save energy and did not require a significant amount of salt to promote the dyeing process.
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危险品化学品经营许可证（带存储）