INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Haiping Yu,Yuanchun Qin,Brian Chen,Fan Song,Shenyang Deng,Lin Wen,Jiaji Pan,Dongying Hu
Fabrics tend to absorb moisture and foster bacterial growth during use, due to surface hydrophilic groups such as hydroxyl groups. Economically and environmentally, extending antibacterial efficacy through hydrophobic treatment proves highly viable. This study employed pressurized impregnation to load ZnO NPs onto polyester (PET) and cotton fabrics, subsequently modified by octadecyltrichlorosilane (OTS) spraying. Post-OTS treatment, the fabrics achieved water contact angles (WCA) of 148.74 ± 0.95° and 145.47 ± 1.13°, respectively. The composite coating displays substrate adaptability by resisting stains from ordinary liquids (tea, milk, coffee, vinegar, cola, etc.) and synthetic blood while maintaining natural breathability. Bacterial eradication is accomplished by synergistic methods, which include electrostatic interactions between ZnO NPs and bacteria, as well as light-induced reactive oxygen species (ROS), giving the fabrics dual antibacterial and hydrophobic properties. The material's antibacterial and hydrophobic qualities remain constant even after acid-base immersion, UV irradiation, mechanical friction, repeated washing, and ultrasonic treatment. This fully fluorine-free approach is environmentally beneficial, increasing fabrics' application possibilities in industries such as fabric manufacturing and medicine.
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JOURNAL OF CONTROLLED RELEASE
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2025
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IF 11.5
Peng Jiang,Yongnian Zeng,Wen Zhang,Jiapeng Lei,Xue Jiang,Wei Li
Reactive oxygen species (ROS)-based therapies have emerged as promising non-antibiotic strategies for treating infected wounds due to their broad-spectrum antimicrobial activity and low risk of resistance development. However, their clinical translation remains limited by low delivery efficiency, poor bacterial targeting, and uncontrolled post-treatment inflammation. To address these challenges, we reported a multifunctional bilayer microneedle patch (LFT/S-MN) designed for efficient bacterial eradication and enhanced wound healing. The patch integrates positively charged lactoferrin-based nanoparticles (LFT NPs) conjugated with a porphyrin sonosensitizer (TAPPFe) in the dissolvable base layer, and a pH-responsive tip layer loaded with the anti-inflammatory agent spermidine (SPD). Upon insertion into infected tissue, the released LFT NPs efficiently target and aggregate negatively charged bacteria, enabling robust ROS generation through synergistic chemodynamic and sonodynamic therapy (CDT/SDT) under ultrasound stimulation. Concurrently, the acidic wound microenvironment triggers the gradual degradation of the tip layer, allowing controlled SPD release for immunomodulation. In a murine P. aeruginosa -infected wound model, the LFT/S-MN patch significantly reduced bacterial load, suppressed inflammation, promoted collagen deposition and neovascularization, and accelerated wound closure. This work presents a promising non-antibiotic strategy for wound disinfection and regeneration through synergistic CDT/SDT and inflammation modulation.
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Journal of Integrative Neuroscience
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2025
·Vol. 24
·期 10
·33497
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IF 2.7
Ying Zhang,Jia Fan,Shanji Nan,Jiaqi Pan,Wanxu Guo,Yizhi Zhang
Background:Alzheimer’s disease (AD) is a severe neurodegenerative disorder that impacts the global impact on the population. Nevertheless, the intricate nature of its pathogenesis has posed significant challenges to drug discovery in this field. This study aimed to verify the therapeutic potential of rubiadin (RB) on AD through both in vivo and in vitro experiments, thereby facilitating translational research for the advancement of AD treatment.Methods:We investigated the neuroprotective effects of RB on AD using both in vivo and in vitro models. Immunohistochemistry and western blot analysis were employed to evaluate inflammatory factors and the Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway in Mo/HuAPP695swe (APP)/PS1-dE9 (PS1) mice and N2a cells.Results:RB enhanced the memory performance of APP/PS1 mice in various tests, including the Morris water maze, step-down and step-through passive avoidance tasks, and novel object recognition. RB reduced the accumulation of Amyloid-beta (Aβ) plaques, as shown by immunohistochemical analysis. It also decreased the expression levels of pro-inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha (TNF-α), while increasing the release of IL-4. Additionally, RB inhibited the NF-κB pathway, as demonstrated by western blot. Moreover, a cell viability test showed that RB protected N2a cells against toxicity caused by Aβ1–42 through a cell viability test. Western blot analysis revealed that neuroinflammation and the NF-κB pathway were inhibited by RB treatment in Aβ1–42-induced N2a cells. Accordingly, RB suppressed the nuclear translocation of NF-κB in Aβ1–42-induced N2a cells.Conclusions:Our results provide experimental evidence supporting the preclinical research and future clinical applications of RB, thereby facilitating the development of new drugs for AD clinical therapy.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Shunji Yang,Weijia Zhang,Longxiao Gao,Jiahui Zhang,Jiarui Li,Xiaoyong Qiu,Fang Wang,Jun Huang,Ningji Gong
In this work, a glucose-responsive hydrogel microneedle has been developed by combining 3D-printed molds with polydimethylsiloxane (PDMS), enabling on-demand adjustment of microneedle shape, height (500–900 μm) and base area (10–1000 mm 2 ). The hydrogel microneedles are fabricated from gelatin methacrylate (GelMA) and poly (ethylene glycol) diacrylate (PEGDA), loaded with glucose oxidase (GOx) as the responsive component and molybdenum disulfide (MoS 2 ) as the catalyst. The GelMA-PEGDA hydrogel for microneedles exhibits a high compressive strength of 1.45 MPa and demonstrates excellent drug-loading and sustained-release capabilities, releasing 55 % of its doxycycline load within 90 min. The GOx/MoS 2 microneedle system shows a high antibacterial efficiency (91.1 %) against Staphylococcus aureus and durable effect (>6 days) in the long-term inhibition zone tests. In vitro cellular experiments demonstrate that these microneedles exhibit low biotoxicity. Further in vivo animal experiments demonstrate promoted wound healing and antibacterial efficacy, with microneedle-treated wounds exhibiting a 19 % higher healing rate compared to the control group after 7 days. This work provides a method for the customized and rapid fabrication of microneedles for various application, demonstrating great potential for reducing bacterial infections and promoting healing in diabetic wounds. Next-generation microneedle research may explore integrated sensing or real-time monitoring to enhance the therapeutic efficacy.
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Journal of Environmental Sciences
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2025
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IF 6.3
Shiyu Yuan,Jingyao Ping,Huayi Chen,Jie Liu,Zewen Tan
The extensive use of chloramphenicol (CAP) in livestock leads the accumulation of CAP in livestock manures, threatening environmental and human health. Therefore, eliminating or reducing CAP concentration in manures before its re-utilization and application through microbial remediation is necessary. In this study, Exiguobacterium sp. CAP4, isolated from the plastisphere in duck manures, was capable of degrading CAP with the biodegradation efficiency of 97.8 % at initial CAP concentration of 5 mg/L within 4 days. A total of twenty-four biotransformation products were determined, including two novel transformation products, TP166 and TP203, enriched the integrity of CAP biodegradation pathways. Furthermore, the biotransformation process was proposed as a detoxifying process through biotransformation products toxicity evaluation. Notably, Exiguobacterium sp. CAP4 successfully colonized in the cow manures after inoculation, and bioaugmented the biodegradation of CAP in virgin cow manures. This study significantly extended our understanding of the CAP biotransformation fate, and provided a promising bacterial strain for bioremediation of CAP containing wastewater in situ .
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BIOSENSORS & BIOELECTRONICS
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2025
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IF 10.5
Qisheng Luo,Chuanhua Zheng,Yongning Huang,Linghuan Liang,Yuanxun Gong,Jiayi Zhang,Qianli Tang,Kai Zhang,Xianjiu Liao
Lead-halide perovskites offer bright electrochemiluminescence (ECL) but suffer from aqueous instability and Pb 2+ safety concerns. We construct a CsPbBr 3 @PDA–Au nanointerface that couples polydopamine (PDA) passivation with Au-assisted charge transfer, integrated into a peptide-to-CRISPR/Cas12a amplification scheme for ultrasensitive detection of matrix metalloproteinase-2 (MMP-2). PDA conformally coats CsPbBr 3 , suppressing trap-mediated quenching and mitigating Pb 2+ release, while providing catechol/amine anchors for a tetrahedral DNA scaffold bearing a ferrocene-labeled hairpin. Upon MMP-2 cleavage of a GPLG↓VRG peptide–DNA chimera, the released strand activates Cas12a trans-cleavage, removing the proximal quencher and switching the interface from “off” to “on.” Under unified processing (baseline subtraction and renormalization), the platform achieves a 5.6 aM detection limit with high selectivity over non-target proteases and excellent inter-device reproducibility (triplicate error bars reported). TEM/HRTEM visualize uniform nanocubes; XPS/FTIR evidence PDA catechol/quinone/amine coordination; XRD confirms phase-pure CsPbBr 3 ; and corrected CV/EIS analyses verify accelerated interfacial kinetics after Au decoration. The sensor retains ∼98 % intra-day and ≥91 % 7-day signal, supporting operational robustness. By integrating interfacial stabilization, catalytic electron transfer, and molecular amplification, this modular design advances eco-conscious, perovskite-based ECL diagnostics for low-abundance protease monitoring and broader clinical bioanalysis.
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Frontiers in Bioengineering and Biotechnology
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2025
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IF 4.8
Shasha Zhou,Xinyu Cao,Xuefei Yin,Jiawen Xu,Kai Gao,Zhenshou Wang
Cysteine-engineered antibodies (THIOMABs) are pivotal in the site-specific conjugation for ADC therapeutics. In this study, THIOMABs were expressed in CHO cell culture and showed a significantly higher proportion of acidic variants compared to traditional antibodies. A dual formation mechanism for acidic species was identified: glutathione (GSH) capping at engineered cysteine sites and traditional post-translational modifications (PTMs) during cell culture. Moreover, it was found that these two mechanisms exhibited overlapping effects on the formation of acidic species, and their simultaneous elimination was required for significant reduction of acidic variants. Consequently, by modulating temperature and pH to reduce PTMs and supplementing with L-cysteine to displace GSH, the proportion of acidic variants was successfully reduced. This study uncovers dual formation mechanisms of acidic species in THIOMABs and provides a practical cell culture approach, effectively reducing acidic variants and enhancing the quality attributes of THIOMABs.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-18
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IF 15.7
Cao Hui,Meng Xin,Ma Yuan,Li Zhe,Xu Xinyu,Fu Jiaqi,Zhang Qingpeng,Wei Hanlin,Liang Peng,Lu Dingyou,Li Jinyu,Yin Baoli,Huan Shuangyan,Zhang Xiao-Bing,Song Guosheng
Atherosclerosis is a leading cause of cardiovascular diseases worldwide. Neutrophil elastase, a key protease secreted by neutrophils, contributes significantly to atherosclerosis by degrading the extracellular matrix and destabilizing the fibrous cap of plaques. However, current detection methods for neutrophil elastase lack the ability to monitor its dynamic changes within plaques in a non-invasive and real-time manner. To overcome this limitation, we develop a neutrophil elastase-activated semiconductor polymer nanoprobe with high selectivity, sensitivity, and resistance to interference. We successfully establish a calibration curve correlating the in vivo photoacoustic signal ratio with neutrophil elastase concentration, enabling quantification of intraplaque neutrophil elastase levels. Importantly, we introduce a vulnerability index to assess plaque instability, allowing the nanoprobe to predict the adverse effects of inflammatory responses on the risk of plaque rupture in ApoE -/- male mice. Additionally, the nanoprobe can visualize dynamic changes in neutrophil elastase during intimal hyperplasia under inflammatory conditions. In conclusion, this nanoprobe provides a tool for elucidating the role of neutrophil elastase in cardiovascular disease progression.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-12
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IF 15.7
Gu Jianfeng,Li Donghui,Ren Yichen,Li JiaHao,Ji Xinyi,Liu Xue,Zhan Weiqing,Zhao Xialian,Wang Quan,Liu Xiewen,Wang Huihui,Zhang Xinmin,Zhu YinBo,Yue Zhao,Wu Heng-An,Chen Yongsheng,Liang Jiajie
Despite high porosity, deer antlers exhibit high strength and toughness from synergistic hierarchical structures. MXene fibers offer promising applications but suffer from poor mechanical properties due to internal voids and weak interfaces. Conventional strengthening removes voids and enhances interlayer interactions, but yields rigid, brittle fibers. Here, we tackle this trade-off by constructing strong and slidable double-interfaces that can cooperate with internal micropores in MXene fibers to mimic the delicate structure of deer antlers. These voids, typically considered defects, instead enhance load-bearing and energy dissipation. At an optimal porosity of ~21%, the MXene fibers achieve high tensile strength, ductility, and toughness of 1060.1 ± 33.5 MPa, 34.2 ± 1.8%, and 136.1 ± 6.5 MJ m - 3 , respectively. Notably, this MXene-matrixed fiber shows elasticity and high durability and is even softer than polymer fibers, while maintaining an electrical conductivity of over 10500 S cm -1 after being coated with silver nanowires. This biomimetic approach enables robust, multifunctional fibers for smart textiles.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-9
·
IF 15.7
Lu Zong-Bin,Peng Shun-Ran,Wang Zhe,Xiong Yu,Xia Lei,Chen Guang,Nie Xuan,Hong Chun-Yan,Zhang Ze,You Ye-Zi
Developing high-performance CO 2 -based polymers is promising to address the challenges of CO 2 sequestration and the environmental impact of petroleum-based plastics. The δ-lactone 3-ethylidene-6-vinyltetrahydro-2H-pyran-2-one and its derivatives, synthesized from CO 2 with 1,3-butadiene, have emerged as very promising CO 2 -derived monomers. However, their general ring-opening polymerizations face challenges with thermodynamics and kinetics, generally resulting in long reaction times, low conversions, and low-molecular-weight polyesters with poor mechanical properties. Herein, we report a dual isomerization-driven cationic ring-opening polymerization (DI-CROP) of a CO 2 -derived thionolactone, 3-ethyl-6-vinyltetrahydro-2H-pyran-2-thione, in which the relayed S/O and vinyl isomerizations significantly enhance polymerization activity, enabling the rapid synthesis of high-molecular-weight CO 2 -based polythioesters, achieving near-quantitative conversion within just a few minutes. Also, the relayed S/O and vinyl isomerizations in DI-CROP can easily migrate C=C substituents on the ring of thionolactone into its backbone. These features further enable the production of sustainable CO 2 -based materials through efficient copolymerization and post-polymerization functionalization. This study enriches the realm of isomerization-driven polymerizations, and provides a new synthetic approach to CO 2 -derived polymeric materials.
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Chemical Science
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2025
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IF 7.4
Taolue Liu,Guojie Gao,Xin Ding,Ying-Ying Li,Yu Wei,Mingxia Guo,Jinxuan Liu,Yan Gao
To elucidate the "reversed proton relay" mechanism in mediating water oxidation under neutral conditions, we designed two Ru(II) complexes: [Ru II (tda-κ-N 3 O)(isoq) 2 ] (Ru1), featuring a coordination-oversaturated pendant carboxylate, and [Ru II (tpc-κ-N 3 O)(isoq) 2 ][PF 6 ] (Ru1'), lacking this structural motif. In Ru1, coordination oversaturation renders the carboxylate dynamically accessible, enabling it to function as a proton switch that extracts protons from the metal center. Density functional theory (DFT) calculations, along with comparative experimental data, confirm that the carboxylate facilitates catalytic switching: in its deprotonated state, it stabilizes key intermediates and promotes O 2 evolution via reversed proton transfer. Online high-resolution mass spectrometry (HRMS) further identifies key intermediates, providing direct experimental evidence for this unconventional proton relay pathway. Collectively, these findings uncover a previously unrecognized mode of proton transfer and offer valuable mechanistic insights for the rational design of efficient water oxidation catalysts under neutral conditions.
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Biomaterials Advances
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2025
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IF 6
Junling Wang,Jingjing Zhu,Yanfang Lu,Aihong Lou,Zhe Cui,Yong Wang,Xianling Wang,Jianglei Qin
Natural polymer-based biodegradable hydrogels can be used to promote the wound healing due to their excellent biocompatibility, tissue adhesion and wound adaptability. In this research, a sprayable hydrogel with good antioxidant, skin adhesion and self-healing property was designed from hydrazide and dopamine functionalized carboxymethyl cellulose (CMC-HD) with oxidized pectin (OPec) cross-linking. The dopamine moiety enhanced antioxidant property of the hydrogel and the MXene nanosheets were composited to take the advantage of the photothermal stimulated antibacterial activit y . The coagulation drug Tranexamic acid (TXA) was loaded and enhance the hemostatic performance of the hydrogel in tail amputation and liver hemorrhage models. In vivo wound repairing experiment in full-thickness skin wound model proved the MXene@TXA/Gel promoted the wound repairing rate under NIR stimulus and this composite hydrogel shows great potential as wound dressing.
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