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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
苹果酸脱氢酶（MDH, EC 1.1.1.37）广泛存在于动物、植物、微生物和培养细胞中，线粒体中 MDH 是TCA 循环的关键酶之一，催化苹果酸形成草酰乙酸，相反，胞浆中 MDH 催化草酰乙酸形成苹果酸。草酰乙酸是重要的中间产物，连接多条重要的代谢途径。因此，MDH 在细胞多种生理活动中扮演着重要的角色，包括线粒体的能量代谢、苹果酸-天冬氨酸穿梭系统、活性氧代谢和抗病性等。根据不同的辅酶特异性，MDH 分为 NAD-依赖的 MDH 和 NADP-依赖的 MDH，细菌中通常只含有NAD-MDH，在真核细胞中，NAD-MDH 分布于细胞质和线粒体中。 本试剂盒提供了一种简单、方便、快速的 mMDH 活性检测方法，适用于检测动物组织、植物组织、细胞和细菌、血清（浆）等样本。其原理是mMDH催化NADH还原草酰乙酸生成苹果酸，导致 340 nm 处光吸收下降。
Malate dehydrogenase (MDH, EC 1.1.1.37) is widely present in animals, plants, microorganisms, and cultured cells. In mitochondria, MDH is one of the key enzymes in the TCA cycle, catalyzing the conversion of malate to oxaloacetate. Conversely, cytoplasmic MDH catalyzes the formation of malate from oxaloacetate. Oxaloacetate is a crucial intermediate that connects multiple important metabolic pathways. Therefore, MDH plays a significant role in various cellular physiological processes, including mitochondrial energy metabolism, the malate-aspartate shuttle system, reactive oxygen species metabolism, and disease resistance. Based on coenzyme specificity, MDH is classified into NAD-dependent MDH and NADP-dependent MDH. Bacteria typically contain only NAD-MDH, while in eukaryotic cells, NAD-MDH is distributed in both the cytoplasm and mitochondria. This kit provides a simple, convenient, and rapid method for detecting mMDH activity, suitable for samples such as animal tissues, plant tissues, cells, bacteria, and serum (plasma). The principle is that mMDH catalyzes the reduction of oxaloacetate by NADH to generate malate, resulting in a decrease in light absorption at 340 nm.
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