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MS培养基(含琼脂、蔗糖, 41.74g/L)
有货
为什么选择此级别
BioReagent,适用于植物细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
MS培养基是当前植物组织培养领域应用最为广泛的基础培养基。该培养基具备高浓度无机盐体系，能够充足供给植物离体组织生长发育所需的各类矿质营养，可有效促进愈伤组织的增殖生长，为植物外植体分化、再生提供稳定的营养支撑。MS培养基的无机营养组分含量科学、配比均衡，完全契合植物细胞生长的营养需求与生理特性，常规植物组培实验中，无需额外添加氨基酸、酪蛋白水解物、酵母提取物、椰子汁等有机附加成分，基础营养可满足绝大多数植物离体培养需求。在应用场景上，MS固体培养基用途广泛，可用于植物愈伤组织的诱导与继代培养，同时适配胚、茎段、茎尖、花药等多种植物外植体的离体培育。对应的MS液体培养基可直接应用于植物细胞悬浮培养，能够有效维持细胞正常生长代谢，培养成功率与稳定性表现优异。
产品成分：
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使用方法
1、配制溶解：精确称取本品41.74 g，加入1 L蒸馏水或去离子水中，充分搅拌至粉末完全溶解，配制成均匀的培养基溶液。
2、pH调节（可选）：如需调节pH值，可在灭菌前使用稀NaOH或者稀HCl溶液进行微调。植物组织培养常用pH范围为5.5～5.8，具体以实际实验方案为准。调节时逐滴添加、边加边搅拌并实时检测pH，避免过度调节。
3、灭菌处理：将配制完成的培养基分装至合适培养容器，于116 ℃高压蒸汽灭菌30 min。
4、冷却使用：灭菌结束后置于洁净无菌环境中自然冷却，全程严格遵守无菌操作规范，即可用于植物组织培养实验。
注意事项
1、本品为植物组织培养全无机盐培养基，建议采用室温水溶解后再进行高压灭菌，不建议加热溶解；灭菌后溶液若出现少量沉淀为正常现象，不影响实验使用。
2、产品需置于室温、避光环境密封保存，有效期3年。
3、本产品仅供科研实验使用，严禁用于医药、临床诊断与治疗、食品、化妆品等相关领域，请勿存放于普通住宅区。
4、因用户非正常操作、违规使用本产品所产生的一切意外与损失，本公司不承担相关责任。
MS medium is the most widely used basal medium in the field of plant tissue culture. Featuring a high-concentration inorganic salt system, this medium fully supplies all mineral nutrients required for the growth and development of isolated plant tissues. It can effectively boost callus proliferation and growth, and provides stable nutritional support for the differentiation and regeneration of plant explants.The inorganic nutrient components of MS medium are scientifically formulated with balanced proportions, which perfectly match the nutritional demands and physiological characteristics of plant cell growth. For routine plant tissue culture experiments, no extra organic additives such as amino acids, casein hydrolysate, yeast extract or coconut water are required, as the basal nutrients can satisfy the in vitro culture demands of the vast majority of plant species.In terms of application scenarios, solid MS medium has versatile uses: it can be applied for callus induction and subculture, and is compatible with the in vitro cultivation of various plant explants including embryos, stem segments, shoot tips and anthers. The corresponding liquid MS medium is directly used for plant cell suspension culture; it efficiently sustains normal cellular growth and metabolism, delivering excellent culture success rate and stability.
Product Composition：
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Instructions for Use：
1. Dissolution: Accurately weigh 41.74 g of this product, add it into 1 L of distilled or deionized water, and stir thoroughly until completely dissolved to prepare a homogeneous medium solution.
2. pH Adjustment (Optional): If pH adjustment is required, finely tune the pH with dilute NaOH or dilute HCl solution before sterilization. The commonly applicable pH range for plant tissue culture is 5.5～5.8, subject to specific experimental protocols. Add the solution dropwise while stirring and monitor the pH in real time to avoid over-adjustment.
3. Sterilization: Dispense the prepared medium into suitable culture vessels and perform high-pressure steam sterilization at 116 ℃ for 30 minutes.
4. Cooling and Application: After sterilization, cool the medium naturally to room temperature in a clean and sterile environment. Use it for plant tissue culture experiments with strict aseptic operation.
Precautions：
1. This product is a full inorganic medium for plant tissue culture. It is recommended to dissolve the powder at room temperature before high-pressure sterilization; heating dissolution is not advised. A small amount of precipitate after sterilization is normal and will not affect the experimental effect.
2. Store the product in a sealed, room temperature and dark environment. The shelf life is 3 years.
3. This product is for research use only. Do not use it for medical treatment, clinical diagnosis, food, cosmetics or other commercial purposes. Do not store it in residential areas.
4. The company shall not be liable for any accidents or losses caused by abnormal or improper use of this product.
规格
别名
MS 基础培养基 | MS 干粉培养基
英文别名
Murashige & Skoog Medium | MS Basal Medium | M&S Medium
规格或纯度
BioReagent, 适用于植物细胞培养
稳定性与储存
Store at room temperature long term (36months). Store in the dark.
英文名称
MS Medium(with agar, sucrose, 41.74g/L)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
产品属性
| pH | 5.7±0.1 |
|---|
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838307 |
分析证书 | M1524134 |
技术文档和文章
溶液计算器
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查看 BioReagent 级别指南 → 查看 Suitable for plant cell culture 级别指南 →相关产品
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产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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