Name: 磁珠法植物DNA提取试剂盒
SKU: M669913
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

🧪

为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

室温。常规运输。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

📚

文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

M669913	Component	96 T	Storage
M669913A	Buffer PLS	50 mL	RT
M669913B	Buffer ZB	55 mL	RT
M669913C	Buffer GW1 (concentrate)	80 mL	RT
M669913D	Buffer TB	10 mL	RT
M669913E	RNase A (10 mg/mL)	0.6 mL	RT
M669913F	Magbeads PN	2×1 mL	RT

产品简介

该试剂盒提供了一种简单、高效的植物DNA提取方案。植物细胞经物理方法破碎后，裂解产物中的DNA在高盐存在时结合于硅基包被的磁珠表面。漂洗后， DNA被洗脱于Buffer TB或去离子水中。提取得率与样品类型以及细胞破碎效果有很大关系，提取得到的DNA可用于二代测序、 PCR检验等下游实验。

自备仪器、试剂

1. CWE2100或CWE9600

2. 96 DW Plate, 8 tip Com, Spin tips pack

3. 无水乙醇

实验前准备以及注意事项

1. 第一次实验前按照试剂瓶标签上的说明向Buffer GW1中加入指定用量的无水乙醇；

2. 客户需配置75%乙醇用于漂洗；

3. Magbeads PN严禁冰冻、高速离心。冰冻、高速离心可能会对Magbeads造成不可逆的损害。

操作步骤

手动操作

1. 植物材料的破碎：

方案1：

1)称取经液氮充分研磨好的新鲜植物样品粉末约50-100 mg或干燥样品粉末约30 mg，迅速转移到预先装有400 µL Buffer PLS和5 µL RNase A（10 mg/mL）的离心管中，迅速颠倒混匀后，瞬时离心，置于恒温混匀仪70℃, 1600 rpm ，10 min。（提取多糖多酚植物在Buffer PLS中加入β巯基乙醇，使其终浓度为5%，可提高核酸提取效果）

方案2：

1）向2.0 mL离心管中加入50 -100 mg新鲜植物材料或20 mg干燥植物材料；

2）用液氮速冻后用玻璃将植物材料充分研磨至粉状；

向离心管中加入400 μL Buffer PLS和5 µL RNaseA（10 mg/mL），迅速颠倒混匀后置于恒温混匀仪70℃, 1600 rpm ，10 min。（提取多糖多酚植物在Buffer PLS中加入β巯基乙醇，使其终浓度为5%，可提高核酸提取效果）

方案3：

1）向2.0 mL离心管中加入50 -100 mg新鲜植物材料或20 mg干燥植物材料；

2）向离心管中加入400 μL Buffer PLS、5 μL RNaseA (10 mg/mL)和1颗钢珠，之后立即将离心管固定于组织破碎仪中震荡破碎；

3）将离心管从组织破碎仪中取出后，瞬时离心，置于恒温混匀仪70℃, 1600 rpm ，10 min。

2. 12,000 rpm (~13,400×g)离心4 min后，转移全部上清至新的1.5 mL离心管中。

3. 向1.5 mL离心管中加入550 μL BufferZB和20 µL Magbeads PN，颠倒混匀30 s，瞬时离心，室温静置5 min，其间颠倒混匀数次。

4. 瞬时离心，将离心管转移至磁力架吸附1min（直至溶液澄清），之后弃去溶液，期间避免接触磁珠。

5. 将离心管从磁力架上取下，加入650 µL Buffer GW1，涡旋混匀10 s，重悬磁珠，之后将离心管固定于25℃、 1600 rpm的恒温混匀仪上震荡混匀2 min或涡旋震荡1 min。

6. 将离心管固定于磁力架上静置1 min，之后充分弃去溶液；

7. 重复步骤5-6；

8. 将离心管从磁力架上取下，加入650 µL 75%乙醇，涡旋混匀10 s，重悬磁珠，之后将离心管固定于25℃、 1600 rpm的恒温混匀仪上震荡混匀2 min或涡旋震荡1 min。

9. 将离心管固定于磁力架上静置1 min，之后充分弃去溶液

10. 重复步骤8-9；

11. 将离心管短暂离心后用移液器再次去除管底溶液，之后室温放置5-10 min 使乙醇充分挥发； 12. 向离心管中加入100 μL Buffer TB，涡旋震荡时磁珠充分悬浮与洗脱液中后将其放于65℃、

1600 rpm的恒温混匀仪上震荡裂解10分钟，或在65℃水浴锅中孵育10 min，期间涡旋震荡4次。

13. 将离心管固定于磁力架上静置2 min，待磁珠充分吸附于离心管侧壁后将洗脱液转移至新的离

心管中-20℃保存备用。

与CWE2100匹配

1. 植物材料的破碎：

方案1：

1)称取经液氮充分研磨好的新鲜植物样品粉末约50-100mg或干燥样品粉末约30mg，迅速转移到预先装有400 µL Buffer PLS和5 µL RNase A（10 mg/mL）的离心管中，迅速颠倒混匀后，瞬时离心，置于恒温混匀仪70℃, 1600 rpm ，10 min。（提取多糖多酚植物在Buffer PLS中加入β巯基乙醇，使其终浓度为5%，可提高核酸提取效果）

方案2：

1) 向2.0 mL离心管中加入50 -100 mg新鲜植物材料或20 mg干燥植物材料；

2) 用液氮速冻后用玻璃将植物材料充分研磨至粉状；

3) 向离心管中加入400 μL Buffer PLS和5 µL RNaseA（10 mg/mL），迅速颠倒混匀后置于恒温混匀仪70℃, 1600 rpm ，10 min。（提取多糖多酚植物在Buffer PLS中加入β巯基乙醇，使其终浓度为5%，可提高核酸提取效果）

方案3：

1) 向2.0 mL离心管中加入50-100 mg新鲜植物材料或20 mg干燥植物材料；

2) 向离心管中加入400 μL Buffer PLS、5 μL RNase A (10 mg/mL)和1颗钢珠，之后立即将离心管固定于组织破碎仪中震荡破碎；

3) 将离心管从组织破碎仪中取出后，瞬时离心，置于恒温混匀仪70℃, 1600rpm ，10 min。 2. 12,000 rpm (~13,400×g)离心4 min后，转移全部上清至96 DW深孔板中；

3. 按下表向96 DW深孔板中加入试剂：

位置	试剂及用量
1&7Colume	Lysate: ALL Buffer ZB: 550 μL Magbeads PN: 20 μL
2&8Colume	Buffer GW1: 650 μL
3&9Colume	Buffer GW1: 650 μL
4&10Colume	75%乙醇: 650 μL
5&11 Colume	75%乙醇: 650 μL
6&12Colume	Buffer TB: 100 μL

4. 将96 DW深孔板以及磁棒套放于CWE2100仪器中，运行Cowin Plant程序。

5. 约50 min后程序运行结束，取出深孔板与磁套。将6&12列中的裂解产物转移至1.5 mL离心管中-20℃保存备用。

与CWE9600匹配

1. 方案1：

1) 称取经液氮充分研磨好的新鲜植物样品粉末约50-100 mg或干燥样品粉末约30 mg，迅速转移到预先装有400 µL Buffer PLS和5 µL RNase A（10 mg/mL）的离心管中，迅速颠倒混匀后，瞬时离心，置于恒温混匀仪70℃, 1600 rpm，10 min。（提取多糖多酚植物在Buffer PLS 中加入5% β巯基乙醇，可提高核酸提取效果）

方案2：

1) 向2.0 mL离心管中加入50-100 mg新鲜植物材料或20 mg干燥植物材料；

2) 用液氮速冻后用玻璃将植物材料充分研磨至粉状；

3) 向离心管中加入400 μL Buffer PLS和5 µL RNase A（10 mg/mL），迅速颠倒混匀后置于恒温混匀仪70℃, 1600rpm，10 min。（提取多糖多酚植物在Buffer PLS中加入5% β巯基乙醇，可提高核酸提取效果）

方案3：

1) 向2.0 mL离心管中加入50-100 mg新鲜植物材料或20 mg干燥植物材料；

2) 向离心管中加入400 μL Buffer PLS、5 μL RNase A (10 mg/mL)和1颗钢珠，之后立即将离心管固定于组织破碎仪中震荡破碎；

3) 将离心管从组织破碎仪中取出后，瞬时离心，置于恒温混匀仪70℃, 1600 rpm ，10 min。

2. 12,000 rpm (~13,400×g)离心4 min后，转移全部上清至96 DW深孔板中；

3. 按下表向96 DW深孔板中加入试剂：

位置	试剂及用量
Plate 1	Lysate: ALL Buffer ZB: 550 μL Magbeads PN: 20 μL
Plate 2	Buffer GW1: 650 μL
Plate 3	Buffer GW1: 650 μL
Plate 4	75%乙醇: 650 μL
Plate 5	75%乙醇: 650 μL
Plate 6	Buffer TB: 100 μL

4. 将96 DW深孔板以及磁棒套放于CWE9600仪器中，运行程序。

5. 约50 min后程序运行结束，取出深孔板与磁套。将Plate 6中的裂解产物转移至1.5 mL离心管中-20℃保存备用。

M669913	Component	96 T	Storage
M669913A	Buffer PLS	50 mL	RT
M669913B	Buffer ZB	55 mL	RT
M669913C	Buffer GW1 (concentrate)	80 mL	RT
M669913D	Buffer TB	10 mL	RT
M669913E	RNase A (10 mg/mL)	0.6 mL	RT
M669913F	Magbeads PN	2×1 mL	RT

Product Introduction
This kit provides a simple and efficient plant DNA extraction solution. After plant cells are physically broken, the DNA in the lysis product binds to the surface of silica coated magnetic beads in the presence of high salt. After rinsing, DNA is eluted in Buffer TB or deionized water. The extraction yield is closely related to the sample type and cell fragmentation effect, and the extracted DNA can be used for downstream experiments such as second-generation sequencing and PCR testing.
Self provided instruments and reagents
1. CWE2100 or CWE9600
2. 96 DW Plate, 8 tip Com, Spin tips pack
3. Anhydrous ethanol
Preparation and precautions before the experiment
1. Before the first experiment, add the specified amount of anhydrous ethanol to Buffer GW1 according to the instructions on the reagent bottle label;
2. The customer needs to configure 75% ethanol for rinsing;
3. Magheads PN strictly prohibits freezing and high-speed centrifugation. Freezing and high-speed centrifugation may cause irreversible damage to Magheads.
Operation steps
Manual operation
1. Crushing of plant materials:
Option 1:
1) Weigh about 50-100 mg of fresh plant sample powder or about 30 mg of dry sample powder thoroughly ground with liquid nitrogen, transfer quickly to a centrifuge tube pre filled with 400 µ L Buffer PLS and 5 µ L RNase A (10 mg/mL), quickly invert and mix, centrifuge instantly, and place in a constant temperature mixer at 70 ℃, 1600 rpm, for 10 minutes. Extract polysaccharides and polyphenols from plants and add them to Buffer PLS β Mercaptoethanol, with a final concentration of 5%, can improve the efficiency of nucleic acid extraction
Option 2:
1) Add 50-100 mg of fresh plant material or 20 mg of dried plant material to a 2.0 mL centrifuge tube; 2) Quickly freeze the plant material with liquid nitrogen and grind it thoroughly to powder using glass;
Add 400 to the centrifuge tube μ L Buffer PLS and 5 µ L RNaseA (10 mg/mL) were quickly mixed upside down and placed in a constant temperature mixer at 70 ℃, 1600 rpm, for 10 minutes. Extract polysaccharides and polyphenols from plants and add them to Buffer PLS β Mercaptoethanol, with a final concentration of 5%, can improve the efficiency of nucleic acid extraction
Option 3:
1) Add 50-100 mg of fresh plant material or 20 mg of dried plant material to a 2.0 mL centrifuge tube;
2) Add 400 to the centrifuge tube μ L Buffer PLS, 5 μ L RNaseA (10 mg/mL) and 1 steel ball, then immediately fix the centrifuge tube in the tissue crusher and shake it to break;
3) After removing the centrifuge tube from the tissue crusher, centrifuge it instantaneously and place it in a constant temperature mixer at 70 ℃, 1600 rpm, for 10 minutes. After centrifugation at 12000 rpm (~13400 × g) for 4 minutes, transfer all supernatant to a new 1.5 mL centrifuge tube.
3. Add 550 to a 1.5 mL centrifuge tube μ Mix L BufferZB and 20 µ L Magheads PN upside down for 30 seconds, centrifuge instantaneously, let stand at room temperature for 5 minutes, and mix upside down several times.
4. Instantaneous centrifugation, transfer the centrifuge tube to a magnetic rack for adsorption for 1 minute (until the solution clears), then discard the solution and avoid contact with the magnetic beads during this period.
5. Remove the centrifuge tube from the magnetic frame, add 650 µ L Buffer GW1, vortex mix for 10 seconds, resuspend the magnetic beads, and then fix the centrifuge tube on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes or vortex shake for 1 minute.
6. Fix the centrifuge tube on a magnetic frame and let it stand for 1 minute, then discard the solution thoroughly;
7. Repeat steps 5-6;
8. Remove the centrifuge tube from the magnetic frame, add 650 µ L of 75% ethanol, vortex mix for 10 seconds, resuspend the magnetic beads, and then fix the centrifuge tube on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes or vortex shake for 1 minute.
9. Fix the centrifuge tube on a magnetic rack and let it stand for 1 minute, then discard the solution thoroughly
10. Repeat steps 8-9;
11. After briefly centrifuging the centrifuge tube, use a pipette to remove the bottom solution again, and then leave it at room temperature for 5-10 minutes to allow the ethanol to fully evaporate;
12. Add 100 to the centrifuge tube μ L Buffer TB, during vortex oscillation, the magnetic beads are fully suspended in the eluent and placed at 65 ℃ Shake and crack on a constant temperature mixer at 1600 rpm for 10 minutes, or incubate in a 65 ℃ water bath for 10 minutes, with 4 vortex oscillations during this period.
13. Fix the centrifuge tube on the magnetic frame and let it stand for 2 minutes. After the magnetic beads are fully adsorbed on the side wall of the centrifuge tube, transfer the eluent to a new centrifuge tube and store it at -20 ℃ for later use.
Matched with CWE2100
1. Crushing of plant materials:
Option 1:
1) Weigh about 50-100mg of fresh plant sample powder or about 30mg of dry sample powder that has been fully ground with liquid nitrogen, and quickly transfer them to a centrifuge tube pre filled with 400 µ L Buffer PLS and 5 µ L RNase A (10 mg/mL). Quickly invert the mixture and centrifuge it instantaneously. Place it in a constant temperature mixer at 70 ℃, 1600 rpm, and 10 minutes. Extract polysaccharides and polyphenols from plants and add them to Buffer PLS β Mercaptoethanol, with a final concentration of 5%, can improve the efficiency of nucleic acid extraction
Option 2:
1) Add 50-100 mg of fresh plant material or 20 mg of dried plant material to a 2.0 mL centrifuge tube;
2) Quickly freeze the plant material with liquid nitrogen and grind it thoroughly to powder using glass;
3) Add 400 to the centrifuge tube μ L Buffer PLS and 5 µ L RNaseA (10 mg/mL) were quickly mixed upside down and placed in a constant temperature mixer at 70 ℃, 1600 rpm, for 10 minutes. Extract polysaccharides and polyphenols from plants and add them to Buffer PLS β Mercaptoethanol, with a final concentration of 5%, can improve the efficiency of nucleic acid extraction
Option 3:
1) Add 50-100 mg of fresh plant material or 20 mg of dried plant material to a 2.0 mL centrifuge tube;
2) Add 400 to the centrifuge tube μ L Buffer PLS, 5 μ L RNase A (10 mg/mL) and 1 steel ball, then immediately fix the centrifuge tube in a tissue crusher and shake to break it;
3) After removing the centrifuge tube from the tissue crusher, centrifuge it instantaneously and place it in a constant temperature mixer at 70 ℃, 1600rpm, for 10 minutes. 2. After centrifugation at 12000 rpm (~13400 × g) for 4 minutes, transfer all supernatant to a 96 DW deep well plate;
3. Add reagents to the 96 DW deep well plate according to the following table:

position	Reagent and dosage
1&7Colume	Lysate: ALL Buffer ZB: 550 μL Magbeads PN: 20 μL
2&8Colume	Buffer GW1: 650 μL
3&9Colume	Buffer GW1: 650 μL
4&10Colume	75%ethanol: 650 μL
5&11 Colume	75%ethanol: 650 μL
6&12Colume	Buffer TB: 100 μL

4. Place the 96 DW deep hole plate and magnetic rod sleeve in the CWE2100 instrument and run the Cowin Plant program.
5. After about 50 minutes, the program ends and the deep hole plate and magnetic sleeve are removed. Transfer the cracking products from columns 6&12 to a 1.5 mL centrifuge tube and store at -20 ℃ for future use.
Matched with CWE9600
Option 1:
1) Weigh about 50-100 mg of fresh plant sample powder or about 30 mg of dry sample powder thoroughly ground with liquid nitrogen, transfer quickly to a centrifuge tube pre filled with 400 µ L Buffer PLS and 5 µ L RNase A (10 mg/mL), quickly invert and mix, centrifuge instantly, and place in a constant temperature mixer at 70 ℃, 1600 rpm, for 10 minutes. Extract polysaccharides and polyphenols from plants and add 5% to Buffer PLS β Mercaptoethanol can improve the efficiency of nucleic acid extraction
Option 2:
1) Add 50-100 mg of fresh plant material or 20 mg of dried plant material to a 2.0 mL centrifuge tube;
2) Quickly freeze the plant material with liquid nitrogen and grind it thoroughly to powder using glass;
3) Add 400 to the centrifuge tube μ L Buffer PLS and 5 µ L RNase A (10 mg/mL) were quickly mixed upside down and placed in a constant temperature mixer at 70 ℃, 1600rpm, for 10 minutes. Extract polysaccharides and polyphenols from plants and add 5% to Buffer PLS β Mercaptoethanol can improve the efficiency of nucleic acid extraction
Option 3:
1) Add 50-100 mg of fresh plant material or 20 mg of dried plant material to a 2.0 mL centrifuge tube;
2) Add 400 to the centrifuge tube μ L Buffer PLS, 5 μ L RNase A (10 mg/mL) and 1 steel ball, then immediately fix the centrifuge tube in a tissue crusher and shake to break it;
3) After removing the centrifuge tube from the tissue crusher, centrifuge it instantaneously and place it in a constant temperature mixer at 70 ℃, 1600 rpm, for 10 minutes.
2. After centrifugation at 12000 rpm (~13400 × g) for 4 minutes, transfer all supernatant to a 96 DW deep well plate;
3. Add reagents to the 96 DW deep well plate according to the following table:

position	Reagent and dosage
Plate 1	Lysate: ALL Buffer ZB: 550 μL Magbeads PN: 20 μL
Plate 2	Buffer GW1: 650 μL
Plate 3	Buffer GW1: 650 μL
Plate 4	75% ethanol : 650 μL
Plate 5	75% ethanol : 650 μL
Plate 6	Buffer TB: 100 μL

4. Place the 96 DW deep hole plate and magnetic rod sleeve in the CWE9600 instrument and run the program.
5. After about 50 minutes, the program ends and the deep hole plate and magnetic sleeve are removed. Transfer the cracking products from Plate 6 to a 1.5 mL centrifuge tube and store at -20 ℃ for later use.

规格

英文名称

Magbead Plant DNA Kit

储存与运输

储存条件

室温

运输条件

常规运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

m669913_magbead plant dna kit.pdf

植物样本核酸提取中CTAB、SDS、TRIzol与柱纯化体系的原理比较与选择

目录：技术文章

探索主题:

SDS 多糖 RNA提取 TRIzol DNA提取植物核酸提取 CTAB 柱纯化多酚

Comparative Principles and Selection of CTAB, SDS, TRIzol, and Column-Based Purification Systems in Nucleic Acid Extraction from Plant Samples

目录：技术文章

探索主题:

SDS TRIzol RNA extraction DNA extraction CTAB plant nucleic acid extraction column purification polysaccharides polyphenols

DNA提取方法比较：酚氯仿法、硅胶柱法、磁珠法、CTAB法与快速裂解法

目录：技术文章

探索主题:

DNA提取磁珠法基因组DNA 酚氯仿抽提硅胶柱法 CTAB法 DNA纯化核酸质量评价

Comparison of DNA Extraction Methods: Phenol-Chloroform Extraction, Silica Column Method, Magnetic Bead Method, CTAB Method, and Rapid Lysis Method

目录：技术文章

探索主题:

DNA extraction Genomic DNA Phenol-chloroform extraction Silica column method Magnetic bead method CTAB method DNA purification Nucleic acid quality assessment

阿拉丁®植物研究相关产品

目录：技术文章

探索主题:

植物组织培养植物学植物激素原生质体遗传转化侵染

Aladdin® Plant Research Related Products

目录：技术文章

探索主题:

Plant tissue culture Genetic transformation Botany Phytohormones Protoplasts Infection

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

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