计算溶液所需的质量、体积或浓度。
BioReagent,无支原体,无菌过滤,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
支原体(Mycoplasma orale)、精氨酸支原体(M. arginini)、猪鼻支原体(M. hyorhinis)和莱氏无胆甾原体(Acholeplasma laidlawii)等。本试剂含有两种杀菌成分:大环内酯类和喹诺酮类抗生素。可有效作用于支原体复制的蛋白合成阶段和 DNA 复制阶段,只需两周即可清除支原体污染,并且对细胞代谢影响小。本试剂对细胞内支原体和细胞外游离的支原体都十分有效,并且对多种革兰氏阳性菌和革兰氏阴性菌也有一定的清除作用。本试剂有效清除支原体的浓度为 12.5-37.5 μg/mL ,一般使用浓度为 25 μg/mL 。具体的工作浓度因支原体的种类不同而有所不同,在实际清除支原体污染时需自行摸索适当的工作浓度。
使用说明:
本产品已经过滤除菌,可以直接使用。使用时请根据实验需要按照适当比例进行稀释,一般稀释100 倍使用。
注意事项:
1. 本品仅供科研使用,不能用于临床治疗,不得用于食品或药品,不得存放于普通住宅内。
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
3. 本试剂可以有效抑制或清除多种支原体,但并不能确保可以抑制或清除实际实验操作中遇到的任何类型的支原体。并且在长期使用本试剂的情况下,不排除会有支原体产生抗性。
Mycoplasma Elimination Reagent is designed to inhibit and eradicate prevalent mycoplasma contamination in cell culture, and can eliminate existing mycoplasma contamination in cells. This reagent effectively suppresses and removes multiple mycoplasma species, including Mycoplasma orale, M. arginini, M. hyorhinis and Acholeplasma laidlawii. The reagent contains two bactericidal components: macrolide and quinolone antibiotics. It targets the protein synthesis and DNA replication stages of mycoplasma replication. Mycoplasma contamination can be eliminated within only two weeks, with minimal impacts on cellular metabolism. The reagent works efficiently against both intracellular mycoplasma and extracellular free mycoplasma. It also exhibits certain eliminating effects on a variety of Gram-positive and Gram-negative bacteria. The effective concentration for mycoplasma eradication ranges from 12.5–37.5 μg/mL, with a typical working concentration of 25 μg/mL. The exact working concentration varies depending on mycoplasma species. Appropriate working concentrations should be optimized empirically for practical mycoplasma decontamination procedures.
Instructions for Use:
This product has been sterile-filtered and ready for direct use. Dilute it at an appropriate ratio according to experimental requirements; a 100-fold dilution is commonly adopted.
Precautions:
1. This product is for research use only. It shall not be used for clinical treatment, food or pharmaceutical applications, and must not be stored in residential premises.
2. Wear a lab coat and disposable gloves during operation for personal safety and health protection.
3. Although this reagent can inhibit or eliminate many mycoplasma species, it cannot guarantee efficacy against all mycoplasma strains encountered in practical experiments. In addition, long-term use may lead to the development of mycoplasma resistance.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | M752153 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Sterile-filtered 级别指南 → 查看 BioReagent 级别指南 → 查看 Mycoplasma free 级别指南 → 查看 for Cell culture 级别指南 →