计算溶液所需的质量、体积或浓度。
BioReagent,无支原体,无菌,用于核酸电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
支原体去除试剂Plus是一种用于清除在培养细胞过程中广泛存在的支原体污染的试剂,可以用于支原体污染的去除和防治,能够有效去除多种支 原体,并且能够预防支原体污染的复发。本产品为多肽类,不像抗生素会影响细胞生长状态并使细胞产生耐药性。
使用说明
1、使用支原体去除试剂Plus处理时,建议现配现用,并保持细胞密度在50%-60%。
2、推荐稀释比例为1:1000。例如10mL的培养基加入10μL的支原体去除试剂Plus混匀。
3、弃去旧的培养基,用PBS将细胞清洗干净,再加入含有支原体去除试剂Plus的新鲜培养基,1天1次,连续处理3天;或者2天1次,连续处理5-6天。若细胞污染非常严重时,需延长处理时间。
4、若细胞对支原体去除试剂Plus敏感,请参考以下推荐参数处理细胞:
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5、细胞支原体去除完成后,加入新鲜培养基,培养基中可添加支原体预防试剂(M752155)预防支原体再次污染。
注意事项
1、本产品仅用于科研,仅作为一种细胞培养基的支原体去除试剂。
2、使用推荐浓度时对细胞毒性极低,目前经测试适用的细胞系:小鼠胚胎干细胞或iPS细胞、人胚胎干细胞或iPS细胞、HEK293、HeLa、MCF7、HepG2、CHO-K1、HCT116、NIH-3T3、Vero、BHK21、HT29、IEC-6、MDCK、PC-12与U20S等。对于特定功能的细胞建议先进行预实验以确定是否对细胞有损伤。
Mycoplasma Removal Agent Plus is designed to eliminate prevalent mycoplasma contamination in cell culture. It serves for both eradication and prevention of mycoplasma pollution, effectively removing various mycoplasma species and preventing recurrent contamination. This product is polypeptide-based; unlike antibiotics, it does not impair cell growth or induce drug resistance in cells.
Instructions for Use:
1. Prepare Mycoplasma Elimination Reagent Plus freshly before use, and maintain cell confluency at 50%–60% during treatment.
2. The recommended dilution ratio is 1:1000. For example, add 10 μL of Mycoplasma Elimination Reagent Plus to 10 mL culture medium and mix thoroughly.
3. Discard spent medium, wash cells thoroughly with PBS, then add fresh medium supplemented with Mycoplasma Elimination Reagent Plus. Treat cells once daily for 3 consecutive days, or once every two days for 5–6 consecutive days. Extend the treatment duration if severe mycoplasma contamination occurs.
4. If cells are sensitive to Mycoplasma Elimination Reagent Plus, treat them per the recommended parameters below:
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5. After complete mycoplasma elimination, replace with fresh culture medium. Mycoplasma Prevention Reagent (Cat. No. M752155) may be supplemented to the medium to prevent secondary mycoplasma contamination.
Precautions:
1. This product is for research use only and exclusively acts as a mycoplasma elimination additive for cell culture medium.
2. It exhibits extremely low cytotoxicity at the recommended working concentration. Tested applicable cell lines include mouse embryonic stem cells and iPSCs, human embryonic stem cells and iPSCs, HEK293, HeLa, MCF7, HepG2, CHO-K1, HCT116, NIH-3T3, Vero, BHK21, HT29, IEC-6, MDCK, PC-12, U2OS, etc. Conduct a preliminary trial for specialized functional cells to verify potential cytotoxicity.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P751569 | |
| 分析证书 | P751569 | |
| 分析证书 | P751569 | |
| 分析证书 | P751569 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Mycoplasma free 级别指南 → 查看 for NA electrophoresis 级别指南 → 查看 Sterile 级别指南 →