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T4 RNA 连接酶
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: 2C5U_B,P00971
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to catalyze 1nmol of 5'-[32P]-(A)12-18 to a phosphatase-indigestible cyclic product within 30 minutes at 37℃.
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T3 RNA 聚合酶
- 种属: 肠杆菌噬菌体T3(Enterobacteria phage T3)
- Accession #: YP_009792932.1,P07659
- 表达系统: E. coli
- 生物活性: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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超快速T4 DNA连接酶
- 种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
- Accession #: P00970
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA in 30min at 16℃ in 20µl of the assay mixture containing 50mM Tris, pH7.5, 10mM MgCl2, 10mM DTT, 1mM ATP, 25µg/ml BSA and a 5'-DNA termini concentration of 0.12µM (300µg/ml).200U equals 1 Weiss unit， Based on Weiss unit, the packaging of this product is 200U and 1000U respectively.
