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基因编辑
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FnCas12a (Cpf1)
- 种属: 土拉弗朗西斯菌亚种.诺维奇达(U112菌株)(Francisella tularensis subsp. novicida (strain U112))
- Accession #: A0Q7Q2.1
- 表达系统: E. coli
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蛋白G-微球菌核酸酶 (pG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A/G-微球菌核酸酶 (pAG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A-微球菌核酸酶（pA-MNase）
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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热敏磷酸酶
- 种属: 南极细菌TAB5(Antarctic bacterium TAB5)
- Accession #: 2W5W_B,Q9KWY4
- 表达系统: E. coli
- 生物活性: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.
