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基因编辑
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蛋白G-微球菌核酸酶 (pG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A/G-微球菌核酸酶 (pAG-MNase)
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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蛋白A-微球菌核酸酶（pA-MNase）
- 种属: 金黄色葡萄球菌(Staphylococcus aureus)
- Accession #: UPI000AAD0772
- 表达系统: E. coli
- 生物活性: One Agarose Gel Unit is defined as the amount of enzyme required to digest 1μg of Lambda DNA in 15 minutes at 37℃, to the extent that the accumulation of low molecular DNA fragments is <400 base pairs as determined by agarose gel electrophoresis. Another Unit is Kunitz Unit. One Kunitz Unit is defined as the amount of enzyme required to release acid soluble oligonucleotides that produce an absorbance increase of O.D. 1.0 at 260nm in 30 minutes at 37 ℃ . 1000 Agarose Gel Units is approximately equal to 100 Kunitz Units.
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AlkB (RNA/DNA 去甲基化酶, Nuclease-free)
- 种属: 大肠杆菌K12株(Escherichia coli (strain K12))
- Accession #: P05050.1
- 表达系统: E. coli
- 生物活性: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
