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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
营养肉汤培养基 (NB) 用于一般细菌的培养,转种,复壮,增菌。
原理和用途:胨和牛肉浸出粉提供氮源、维生素、矿物质和氨基酸等生长必需的营养物质;氯化钠维持均衡的渗透压。
配制方法:称取本品18.0g,置于1L纯化水中。加热搅拌至完全溶解,分装,115℃高压灭菌30min,放置冷却,备用。
适用范围:
| 应用 | 类别 |
| 增菌 | 中温好氧菌 |
配方 (g/L):
| 胨 | 10.0g |
| 牛肉浸出粉 | 3.0g |
| 氯化钠 | 5.0g |
| 最终pH值 (25℃) | 7.2±0.2 |
质量控制:
| 测试项目 | 标准指标 |
| 外观 | 粉末状 |
| 干粉颜色 | 淡黄色 |
| 制备好培养基颜色 | 淡黄色透明液体 |
| 最终pH值 (25°C) | 7.2±0.2 |
微生物测试:
根据《中华人民共和国药典》检测:金黄色葡萄球菌、铜绿假单胞菌、枯草芽孢杆菌、大肠埃希氏菌和乙型副伤寒沙门氏菌。
| 质控菌株及编号 | 生长情况/菌落特征 |
| 金黄色葡萄球菌 CMCC(B)26003 | 生长良好,浑浊 |
| 铜绿假单胞菌 CMCC(B)10104 | 生长良好,浑浊 |
| 枯草芽孢杆菌 CMCC(B)63501 | 生长良好,浑浊、液体表面有少量皱醭 |
| 大肠埃希氏菌 CMCC(B)44102 | 生长良好,浑浊 |
| 乙型副伤寒沙门氏菌 CMCC(B)50094 | 生长良好,浑浊 |
接种条件:10-100CFU;
培养条件:35℃/24小时。
参考标准:
《中华人民共和国药典》
注意事项:
1. 称取培养基时,请做好必要的防护措施,以免引起身体不适。
2. 培养基应置于避光、干燥处保存,使用后应立即旋紧瓶盖,以免吸潮结块。
3. 使用后的培养基应在121°C下高压灭菌30分钟后处理。
4、本品不得用于临床检测。
Nutrient Broth Medium (NB) is used for the cultivation, sub‑culturing, rejuvenation and enrichment of common bacteria.
Principle and Purpose: Peptone and beef extract powder supply essential nutrients for bacterial growth, including nitrogen sources, vitamins, minerals and amino acids. Sodium chloride maintains balanced osmotic pressure.
Preparation Method: Weigh 18.0 g of this product and dissolve it in 1 L of purified water. Heat and stir until completely dissolved, dispense into containers, autoclave at 115 °C for 30 min, cool and set aside for later use.
Scope of Application:
| Application | Category |
| Enrichment | Mesophilic aerobic bacteria |
Formula (g/L):
| Peptone | 10.0g |
| Beef Extract Powder | 3.0g |
| Sodium Chloride | 5.0g |
| Final pH (25℃) | 7.2±0.2 |
Quality Control:
| Test Item | Specification |
| Appearance | Free-flowing powder |
| Colour of dehydrated medium | Pale yellow |
| Colour of prepared medium | Pale yellow clear liquid |
| Final pH (25 °C) | 7.2±0.2 |
Microbiological Testing:
Test for Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Salmonella paratyphi‑B in accordance with the Pharmacopoeia of the People's Republic of China.
| QC Strain and Strain No. | Growth Performance / Characteristics |
| Staphylococcus aureus CMCC(B)26003 | Good growth, turbidity |
| Pseudomonas aeruginosa CMCC(B)10104 | Good growth, turbidity |
| Bacillus subtilis CMCC(B)63501 | Good growth, turbidity, with a small amount of wrinkled pellicle on the liquid surface. |
| Escherichia coli CMCC(B)44102 | Good growth, turbidity |
| Salmonella Paratyphi‑B CMCC(B)50094 | Good growth, turbidity |
Inoculation level: 10-100CFU
Incubation conditions: 35°C for 24 hours
Reference Standard:
Chinese Pharmacopoeia
Precautions:
1. Take necessary protective measures when weighing the medium to avoid physical discomfort.
2. The medium shall be stored in a dark and dry place. Tighten the bottle cap immediately after use to prevent moisture absorption and caking.
3. The used medium shall be autoclaved at 121℃ for 30 minutes before disposal.
4. This product is not intended for clinical testing.
| pH | 7.0-7.4 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N1523355 |
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