多聚赖氨酸溶液英文名为Poly-L-lysine Solution简称PLL，Poly-L-lysine为Poly-L-lysine hydrobromide，分子式为L-Lys-(L-Lys)n-L-Lys•xHBr，分子量为150,000-300,000，CAS Number 25988-63-0。
PLL是一种粘附剂，常用于载玻片的包被,可以直接稀释后用于细胞或组织培养方面的实验，分子量大于70,000的多聚赖氨酸可以促进细胞贴壁生长，该试剂经RNase free处理，可以用于促进细胞的贴壁生长和核酸杂交，制备好的载玻片可4℃保存半年。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤(仅供参考)：
1、用于细胞培养
①根据实验需要Poly-L-lysine Solution稀释至适当浓度溶液后即可使用；不同的细胞，Poly-L-lysine Solution包被 (Coating)的时间和浓度，甚至稀释液的选择有所不同，请自行参考相关文献进行适当的包被。
②Poly-L-lysine Solution用于细胞培养时，包被至少5min，有些实验需要包被1-2h，有些情况则需要包被过夜。
③包被完成后，吸Poly-L-lysine Solution，自然干燥培养器皿，至肉眼观察完全干燥，通风橱内吹风数分钟即可完成干燥，对于有些实验则需要干燥2h或更长时间；干燥时间较长通常会更加有利于后续的细胞粘附。
④进行细胞培养，也可以用水、PBS或培养液等适当溶液润洗后再进行细胞培养。
2、用于核酸杂交
①方法一：取事先准备好的载玻片或盖玻片经160℃冷却至室温，在Poly-L-lysine Solution上下浸蘸几下，自然干燥，4℃备用，亦可室温保存1个月。
②方法二：Poly-L-lysine Solution涂于玻片上，自然干燥后即可使用，可用于细胞涂片和切片。
③方法三：滴加5-10μl的Poly-L-lysine Solution至玻片上，用另一盖玻片以血涂片方法推片或用另一玻片紧贴于其上，相互摩擦以使两玻片相对的一面涂布上明胶包被溶液。
注意事项：
1、Poly-L-lysine可以被某些细胞所消化并吸收，摄入过多的Poly-L-lysine会产生一定的细胞毒性。
2、避免反复冻融，如果大量使用，可以取适量溶液置于4℃保存，6个月有效。
3、浸蘸Poly-L-lysine Solution时，务必使玻片完全浸入液体中，否则易使包被不完全产生样本脱落现象。
4、干燥过程中注意避免尘埃污染。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Poly-L-lysine Solution, abbreviated as PLL, refers to Poly-L-lysine hydrobromide in its chemical form. Its molecular formula is L-Lys-(L-Lys)n-L-Lys•xHBr, with a molecular weight ranging from 150,000 to 300,000, and its CAS Number is 25988-63-0.
PLL is an adhesive commonly used for coating glass slides. It can be directly diluted and applied to experiments involving cell or tissue culture. Poly-L-lysine with a molecular weight greater than 70,000 can promote the adherent growth of cells. This reagent has been treated with RNase-free processing, making it suitable for enhancing cell adhesion and nucleic acid hybridization. The prepared slides can be stored at 4℃ for up to six months. This reagent is for research use only and is not intended for clinical diagnosis or any other purposes.
Operating Procedures (For Reference Only)
1. For Cell Culture
① Dilute the Poly-L-lysine Solution to an appropriate concentration according to experimental requirements before use. The coating time, concentration of Poly-L-lysine Solution, and even the selection of diluent may vary for different cell types. Please refer to relevant literature to perform coating appropriately.
② When using Poly-L-lysine Solution for cell culture, the coating duration should be at least 5 minutes. Some experiments require coating for 1-2 hours, while others may need overnight coating.
③ After coating, aspirate the Poly-L-lysine Solution and air-dry the culture vessel until completely dry as observed by the naked eye. Drying can be accomplished by blowing air in a fume hood for several minutes; certain experiments may require drying for 2 hours or longer. Generally, an extended drying time is more conducive to subsequent cell adhesion.
④ Proceed with cell culture. Alternatively, the coated vessel can be rinsed with an appropriate solution such as water, PBS, or cell culture medium prior to cell seeding.
2. For Nucleic Acid Hybridization
① Method 1: Take the pre-prepared glass slides or coverslips, cool them to room temperature after heating at 160℃, dip them into and out of the Poly-L-lysine Solution several times, air-dry them, and store at 4℃ for later use. They can also be stored at room temperature for up to one month.
② Method 2: Apply the Poly-L-lysine Solution onto the slides and air-dry; the coated slides are ready for use in cell smears and tissue sections.
③ Method 3: Add 5-10 μl of Poly-L-lysine Solution onto a slide, then use another coverslip to spread the solution using the blood smear technique, or press another slide tightly against it and rub them against each other to coat the facing surfaces with the solution.
Precautions
1. Poly-L-lysine can be digested and absorbed by certain cell types. Excessive intake of Poly-L-lysine may induce cytotoxicity.
2. Avoid repeated freezing and thawing. For large-volume usage, dispense an appropriate amount of the solution and store it at 4℃, with a validity period of 6 months.
3. When dipping slides into the Poly-L-lysine Solution, ensure that the slides are fully submerged in the liquid; otherwise, incomplete coating may cause sample detachment.
4. Prevent dust contamination during the drying process.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. Please use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
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