计算溶液所需的质量、体积或浓度。
丙酮酸激酶 (PK)
有货
为什么选择此级别
冻干粉,天然,生物活性,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
丙酮酸激酶（PK）是糖酵解通路酶，存在 L、R、M1、M2 四种同工酶。L 型同工酶分布于糖异生组织，主要为肝脏；R 型、M1 型分别分布于成人骨骼肌、心脏、脑以及红细胞；M2 型定位于细胞核内。
反应式
ATP + 丙酮酸 ⇌ ADP + 磷酸烯醇式丙酮酸
作用机理
丙酮酸激酶催化磷酸烯醇式丙酮酸（PEP）上的磷酸基团转移至 ADP；该反应生成一分子丙酮酸与一分子 ATP。
应用
与配套酶偶联，用于 ADP、ATP、丙酮酸或磷酸烯醇式丙酮酸（PEP）的酶法检测。
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells.
Reaction
ATP + pyruvate = ADP + phosphoenolpyruvate
Action
Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP.
This transfer yields one molecule of pyruvate and one molecule of ATP.
Application
Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes.
规格
产品名称
丙酮酸激酶 (PK)
别名
ATP：丙酮酸盐 2-O-磷酸转移酶
英文别名
ATP:pyruvate 2-O-phosphotransferase | pyruvate kinase | phosphoenolpyruvate kinase | phosphoenol transphosphorylase | PKM | PKM2
规格或纯度
生物活性, 天然, 高性能, 冻干粉, 来源于兔肌肉; ≥200 U/mg enzyme powder
生化机理
Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Isoform M2: Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity. In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase. Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase. Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription. Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis. Promotes the expression of the immune checkpoint protein CD274 in BMAL1-deficient macrophages. May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs. Plays a role in caspase independent cell death of tumor cells. Isoform M1: Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth. In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity. There are 4 isozymes of pyruvate kinase in mammals (L, R, M1, M2) encoded by 2 different genes: PKLR and PKM. The L and R isozymes are generated from the PKLR by differential splicing of RNA; the M1 and M2 forms are produced from the PKM gene by differential splicing. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells.
Post-translational: ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Isoform M2: Acetylated by EP300, leading to impair phosphoenolpyruvate substrate-binding and promote its homodimerization and subsequent translocation to the nucleus. Deacetylation by SIRT6 promotes its nuclear export into the cytoplasm, leading to suppress its nuclear localization and oncogenic function. Isoform M2: S-nitrosylation at Cys-423 and Cys-424 inhibits homotetramerization and pyruvate kinase activity. S-nitrosylation is indirectly inhibited by AKR1A1 which degrades S-nitroso-CoA, a cofactor required to S-nitrosylate proteins. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
生物活性
≥200 U/mg enzyme powder
种属
兔(Rabbit)
来源
天然
预测分子量
237 kDa (as a tetramer of four equal subunits 57 kDa)
CAS编号和信息
9001-59-6
酶学委员会编号
EC 2.7.1.40
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from Tris-Cl, pH 7.7.
浓度
来源于兔肌肉; ≥200 U/mg enzyme powder
复溶
以 0.9% 生理盐水复溶；采用含 1% BSA 的 TBS 缓冲液做后续稀释。复溶后分装，‑20 ℃冷冻储存。
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（36个月）；复溶后建议分装，避免反复冻融。
单位定义
1 个酶活单位 (U) 定义：在 37 ℃、pH 7.6 条件下，每分钟催化 1.0 μmol 磷酸烯醇式丙酮酸转化为丙酮酸所需的酶量。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333583 |
分析证书 | P1510309 |
| ZJ26F0333584 |
分析证书 | P1510309 |
| ZJ26F0333585 |
分析证书 | P1510309 |
技术文档和文章
溶液计算器
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