BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丙酮酸(Pyruvic acid，PA)又称2-氧代丙酸，是参与整个生物体基本代谢的中间产物之一，可通过乙酰辅酶A和三羧酸循环实现体内糖、脂肪和氨基酸间的互相转化，丙酮酸在三大营养物质的代谢联系中起着重要的枢纽作用，丙酮酸是糖无氧代谢的产物，科研工作者常将丙酮酸和乳酸一起研究，并用二者的比值推算循环衰竭的程度。丙酮酸检测可采用乳酸脱氢酶催化法或二硝基苯肼比色法。 该试剂盒的检测原理是在酸性条件下，丙酮酸与二硝基苯肼反应，生成丙酮酸-二硝基苯腙复合物，后者经氧化呈棕红色，通过分光光度比色法测定520nm处吸光度，据此通过与标准曲线对比可以计算出PA水平。可用于检测植物、细胞或组织、血清等样品中内源性的丙酮酸含量，尤其适用植物样品丙酮酸含量的检测。本产品仅用于科研领域，不宜用于临床诊断或其他用途。
Pyruvic acid (PA), also known as 2-oxopropanoic acid, is a key intermediate in fundamental metabolic processes throughout living organisms. It serves as a crucial link in the interconversion of carbohydrates, fats, and amino acids via acetyl-CoA and the tricarboxylic acid (TCA) cycle. As the end product of anaerobic glycolysis, pyruvate is often studied alongside lactate in research, with their ratio used to assess the degree of circulatory failure. Pyruvate detection can be performed using methods such as the lactate dehydrogenase (LDH) catalytic assay or the dinitrophenylhydrazine colorimetric method. The detection principle of this kit is based on the reaction of pyruvic acid with dinitrophenylhydrazine under acidic conditions to form a pyruvate-dinitrophenylhydrazone complex. This complex develops a brownish-red color upon oxidation. The absorbance is measured at 520 nm using spectrophotometry, and the PA concentration is calculated by comparison with a standard curve. This kit is suitable for detecting endogenous pyruvate levels in samples such as plants, cells or tissues, and serum, with particular applicability for plant samples. This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.
危险品化学品经营许可证（带存储）