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静脉血离体后至完全凝固所需的时间即为凝血时间，它是反映内源性凝血系统各凝血因子活性的筛选实验，凝血酶原时间 (PT) 是在待检血浆中加入过量的组织凝血活酶和钙离子，使凝血酶原转变为凝血酶，后者使纤维蛋白原转变为纤维蛋白，计算缺乏血小板的血浆凝固所需的时间，以ISI为参考。
凝血酶原时间 (PT) 检测试剂盒 (一期法) 用于检测人、动物血液的血浆凝血酶原时间的测定，不仅反映凝血酶原水平，也反映因子Ⅴ、Ⅶ、Ⅹ和纤维蛋白原在血浆中的水平，是外源性凝血系统的筛选实验试剂盒。该试剂盒仅用于科研领域，不宜用于临床诊断或其他用途。
组分与储存：
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自备材料：
1. 枸橼酸钠抗凝剂采血管 (可选)、采血针、止血带
2. 离心机、计时器或秒表、水浴锅
操作步骤（仅供参考）：
1. 制备待测血浆：取新鲜待测静脉血与柠檬酸钠抗凝剂 (109mM) 按9:1混合，轻轻混匀，3000r/min离心10-15min，收集上层液 (缺乏血小板的血浆)，转移至塑料试管或离心管，以防止血小板被激活；同时应设正常对照血浆。
2. 取待测血浆0.07ml与PT 凝血活酶溶液0.14ml，分别置于37°C水浴中，温育3min。
3. 将待测血浆 (包括正常对照血浆) 与PT凝血活酶溶液混合，立即开动秒表计时，不断轻轻倾斜试管，记录至液体停止流动所需的时间。重复2-3次取平均值，即为凝血酶原时间 (PT)。
凝血酶原时间比值 (PTR) = 待检血浆的 PT/正常参比血浆的 PT
国际标准化比值 (INR) = PTRISI
参考区间：
PT值：(正常人静脉血) 10-14s，待测者的测量值较正常对照值延长3s以上具有临床意义。该结果仅供参考，由于试剂批次、仪器、样本、操作人员等不同，会导致参考值不同，建议各实验室建立自己的参考范围。
ISI值：<2.0s
注意事项：
1. 以上操作，应同时测定正常对照。
2. 可采用枸橼酸钠抗凝管采集血浆，不能用EDTA、肝素、草酸盐等作为抗凝剂；获得样本后应及时检测，一般室温不应超过1h，4℃不应超过4h，一般不建议低温长期保存。
3. 采血必须使用一次性塑料注射器或硅化玻璃注射器，贮血必须使用塑料试管或硅化玻璃管，不宜使用普通玻璃容器，避免凝血因子活化。
4. 抽血要顺利，抗凝要充分。样本采集应避免溶血及组织污染。
5. 分离血浆应在3000g离心10min以上，务必去除血小板。
6. 血浆预温不宜超过 5min，PT 凝血活酶溶液预温不宜超过15min。
7. 应精确控制孵育温度在37±1℃，过高或过低均会使PT延长。
8. 操作时光线要充足，凝血酶原时间的终点计算以出现混浊的初期凝固为准。
9. 红细胞比容 (Hct) 不在20-55%时，抗凝剂与血液的比例应按以下公式调整：
抗凝剂 (ml)=(100%-Hct)x血液 (ml)x0.00185
10. 本产品仅用于科研领域，不得用于临床诊断或其他用途。
11. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Clotting time refers to the time required for venous blood to clot completely after being drawn from the body. It is a screening assay reflecting the activity of various coagulation factors in the intrinsic coagulation system. Prothrombin Time (PT) is determined by adding excess tissue thromboplastin and calcium ions to test plasma. Prothrombin is converted into thrombin, which further transforms fibrinogen into fibrin. The time required for platelet-poor plasma to coagulate is recorded, with the International Sensitivity Index (ISI) as the reference.
Prothrombin Time (PT) Assay Kit (One-stage Method) is used to measure prothrombin time in plasma from human and animal blood. It reflects not only the level of prothrombin but also the plasma concentrations of Factor V, Factor VII, Factor X and fibrinogen. This kit serves as a screening reagent kit for the extrinsic coagulation system. For Research Use Only. Not intended for clinical diagnosis or other applications.
Contents & Storage:
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Materials to Be Prepared:
1. Sodium citrate anticoagulant blood collection tubes (optional), blood collection needles, tourniquets
2. Centrifuge, timer or stopwatch, water bath
Operating Procedures (For Reference Only):
1. Preparation of test plasma: Mix fresh venous blood sample with 109 mM sodium citrate anticoagulant at a ratio of 9:1, invert gently to homogenize. Centrifuge at 3000 r/min for 10–15 min. Collect the supernatant (platelet-poor plasma) and transfer into plastic test tubes or centrifuge tubes to avoid platelet activation. Normal control plasma shall be prepared in parallel.
2. Pipette 0.07 mL test plasma and 0.14 mL PT Thromboplastin Solution separately, incubate both at 37 °C water bath for 3 min.
3. Combine test plasma (including normal control plasma) with PT Thromboplastin Solution, start the stopwatch immediately. Tilt the tube gently and continuously. Record the time until the liquid stops flowing. Repeat the test 2–3 times and calculate the average value as Prothrombin Time (PT).
Prothrombin Time Ratio (PTR) = PT of test plasma / PT of normal reference plasma
International Normalized Ratio (INR) = PTRISI
Reference Intervals:
PT value (venous blood from healthy subjects): 10–14 s. A measured value ≥3 s longer than the normal control value is considered clinically significant. The above values are for reference only. Reference ranges may vary due to reagent batches, instruments, samples and operators. Each laboratory is recommended to establish its own reference interval.
ISI value: ＜2.0
Precautions:
1. A normal control sample must be tested simultaneously in all operations.
2. Plasma shall be collected using sodium citrate anticoagulant tubes. EDTA, heparin, oxalate and other anticoagulants are not allowed. Samples should be tested promptly after collection; storage at room temperature shall not exceed 1 h, and storage at 4 °C shall not exceed 4 h. Long-term low-temperature storage is generally not recommended.
3. Blood collection must use disposable plastic syringes or siliconized glass syringes. Blood storage must adopt plastic tubes or siliconized glass tubes. Ordinary glass containers shall not be used to prevent activation of coagulation factors.
4. Blood drawing should be smooth with sufficient anticoagulation. Avoid hemolysis and tissue contamination during sampling.
5. Plasma separation requires centrifugation above 3000 g for more than 10 min to ensure complete removal of platelets.
6. Plasma pre-incubation shall not exceed 5 min; pre-incubation of PT Thromboplastin Solution shall not exceed 15 min.
7. Incubation temperature shall be precisely controlled at 37±1 °C; either higher or lower temperature will prolong PT.
8. Sufficient light is required during operation. The endpoint of prothrombin time is defined as the initial visible turbidity and clot formation.
9. When hematocrit (Hct) is outside the range of 20–55%, the ratio between anticoagulant and blood shall be adjusted according to the formula below:
Anticoagulant volume (mL) = (100% − Hct) × Blood volume (mL) × 0.00185
10. This product is For Research Use Only. Not intended for clinical diagnosis or any other applications.
11. For your safety and health, wear a lab coat and disposable gloves during operation.
| 项目号 | 物理外观 | 组件 | 50T | 储存 |
| P1522036A | 液体 | 柠檬酸钠抗凝剂 (109mM) | 20 mL | 2-8℃. |
| P1522036B | 液体 | PT 凝血活酶溶液 | 15 mL | 2-8℃. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838324 |
分析证书 | P1522036 |
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