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静脉血离体后至完全凝固所需的时间即为凝血时间，它是反映内源性凝血 系统各凝血因子活性的筛选实验。在凝血酶作用下，待测血浆中的纤维蛋白原转变为纤维蛋白，当待测血浆中抗凝物质增多时，凝血酶时间会相应延长。
凝血酶时间(TT)检测试剂盒用于检测人、动物血浆凝血酶时间， 凝血酶时间延长见于肝素增多或类肝素抗凝物质存在，纤维蛋白(原)降解产物 (FDP)增多以及低(无)纤维蛋白原血症；凝血酶时间缩短见于血样本有微小凝血块或存在钙离子。该试剂盒仅用于科研领域，不宜用于临床诊断或其他用途。
产品组分
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自备材料
1)枸橼酸钠抗凝剂采血管、采血针、止血带
2)离心机、计时器或秒表、水浴锅
操作步骤(仅供参考)
1)制备待测血浆：取新鲜待测静脉血 1.8 mL，与 0.2 mL柠檬酸钠抗凝剂(109 mM)按 9:1 混合，轻轻颠倒混匀。(或用含有 1/10 体积的柠檬酸钠抗凝剂的塑料管或硅化玻璃管采血)
2)3000 rpm（或 2500 g）离心 10~15 min，收集上层液(缺乏血小板的血浆)，转移至塑料试管或离心管，以防止血小板被激活；同时应设正常对照血浆。
3)取待测抗凝血浆 0.1 mL，置于 37℃水浴中，温育 5 min。
4)加入凝血酶溶液 0.1 mL，记录凝固时间。重复 2～3 次取平均值。
参考区间
14.0~21.0 s，若超过正常对照 4s 以上者为异常。
注意事项
1)以上操作，应同时测定正常对照。
2)样本应避免溶血或组织液污染。
3)获得样本后，应及时检测，一般不应超过1 h，置于冰箱中也不应超过4 h。
4)采血必须使用一次性塑料注射器或硅化玻璃注射器，贮血必须使用塑料试管或硅化玻璃管，不宜使用普通玻璃容器，避免凝血因子活化。
5)采血时止血带不可束缚过紧，而且不应超过5分钟，否则导致凝血及纤溶因子活化。
6)分离血浆应在 3000 rpm 离心 10~15 min，务必去除血小板。
7)本实验不宜用肝素、EDTA 或草酸盐作为抗凝剂。
8)测定温度在 36.5~38.5 ℃，过低或过高均可使 TT 延长。
9)本产品仅用于科研领域，不得用于临床诊断或其他用途。
10)为了您的安全和健康，请穿实验服并戴一次性手套操作。
Clotting time is defined as the time required for venous blood to fully coagulate after being drawn from the body. It serves as a screening assay reflecting the activity of various coagulation factors in the intrinsic coagulation pathway. Under the action of thrombin, fibrinogen in the test plasma is converted into fibrin. When anticoagulants increase in the test plasma, the thrombin time will be prolonged accordingly.
The Thrombin Time (TT) Assay Kit is used to determine the thrombin time of human and animal plasma. Prolonged thrombin time is observed in cases of elevated heparin or heparin-like anticoagulants, increased fibrin(ogen) degradation products (FDP), and hypo(a)fibrinogenemia. Shortened thrombin time may occur when microclots exist in blood samples or calcium ions are present. This kit is intended for research purposes only and shall not be used for clinical diagnosis or other applications.
Component
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Materials to be prepared by users
1)Sodium citrate anticoagulant blood collection tubes, blood collection needles, tourniquets
2)Centrifuge, timer or stopwatch, water bath
Operating Procedures (For reference only)
1)Preparation of test plasma: Collect 1.8 mL fresh venous blood sample, mix gently with 0.2 mL sodium citrate anticoagulant (109 mM) at a volume ratio of 9:1 with gentle inversion. (Alternatively, collect blood using plastic tubes or siliconized glass tubes pre-filled with 1/10 volume of sodium citrate anticoagulant.)
2)Centrifuge at 3000 rpm (or 2500 g) for 10~15 min. Collect the supernatant (platelet-poor plasma) and transfer it into plastic test tubes or centrifuge tubes to avoid platelet activation. Normal control plasma shall be set up simultaneously.
3)Pipette 0.1 mL anticoagulated test plasma and incubate in a 37 °C water bath for 5 min.
4)Add 0.1 mL thrombin solution and record the clotting time. Repeat the test 2-3 times and calculate the average value.
Reference Range
14.0~21.0 s. Results exceeding the normal control value by more than 4 s are considered abnormal.
Precautions
1)A normal control shall be tested in parallel for all operations described above.
2)Samples should be protected from hemolysis and tissue fluid contamination.
3)Assays should be performed promptly after sample collection, preferably within 1 h. Storage in a refrigerator must not exceed 4 h.
4)Disposable plastic syringes or siliconized glass syringes must be used for blood collection; plastic tubes or siliconized glass tubes are required for blood storage. Ordinary glass containers are not recommended to prevent activation of coagulation factors.
5)The tourniquet should not be tied too tightly during blood collection, and the constriction time should not exceed 5 minutes; otherwise, coagulation and fibrinolytic factors may become activated.
6)Centrifuge samples at 3000 rpm for 10–15 min for plasma separation to ensure complete removal of platelets.
7)Heparin, EDTA or oxalate should not be used as anticoagulants in this assay.
8)The testing temperature should be maintained at 36.5~38.5 °C. Temperatures below or above this range can prolong TT values.
9)This product is for research purposes only and must not be used for clinical diagnosis or other applications.
10)Wear a lab coat and disposable gloves during operation for your personal safety and health.
T1522037 | 组件 | 物理外观 | 50 T | 100 T | 储存 | 单次测试使用量 |
T1522037A | 柠檬酸钠抗凝剂 (109mM) | 无色液体 | 10 mL | 20 mL | 2-8 ℃ | 0.1 mL |
T1522037B | 凝血酶溶液 | 无色液体 | 10 mL | 20 mL | 2-8 ℃ | 0.1 mL |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838344 |
分析证书 | T1522037 |
| ZJ26F0838345 |
分析证书 | T1522037 |
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