丙酮酸氧化酶是一种焦磷酸硫胺素依赖型的氧化酶,可以催化丙酮酸氧化脱羧生成乙酰磷酸、二氧化碳和水。 丙酮酸氧化酶是细菌代谢中的一种重要酶,可增加 ATP 的产生。此外,可用于天冬氨酸氨基转移酶和丙氨酸氨基转移酶活性检测。
Pyruvate oxidase is a thiamine pyrophosphate-dependent oxidase that catalyzes the oxidative decarboxylation of pyruvate to produce acetyl phosphate, carbon dioxide, and water. Pyruvate oxidase is an important enzyme in bacterial metabolism and can enhance ATP production. Additionally, Pyruvate oxidase can be used for the determination of aspartate aminotransferase and alanine aminotransferase activities.
pyruvate + phosphate + O₂ = acetyl phosphate + CO₂ + H₂O₂. A flavoprotein (FAD) requiring thiamine diphosphate. Two reducing equivalents are transferred from the resonant carbanion/enamine forms of 2-hydroxyethyl-thiamine-diphosphate to the adjacent flavin cofactor, yielding 2-acetyl-thiamine diphosphate (AcThDP) and reduced flavin. FADH₂ is reoxidized by O₂ to yield H₂O₂ and FAD and AcThDP is cleaved phosphorolytically to acetyl phosphate and thiamine diphosphate.
生物活性
≥5 U/mg enzyme powder; ≥10 U/mg protein
来源
重组表达
CAS编号和信息
9001-96-1
酶学委员会编号
EC 1.2.3.3
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥5 U/mg enzyme powder; ≥10 U/mg protein
复溶
Reconstitute in water to a concentration of 10mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(12个月);长期储存-20℃(24个月);复溶后建议分装,避免反复冻融。
单位定义
One unit will produce 1.0 micromole of hydrogen peroxide per minute at pH 5.7 at 37 Deg C during the conversion of pyruvate and phosphate to acetyl phosphate and carbon dioxide.