This product is an alkaline phosphatase from Escherichia coli, and is one of the most active alkaline phosphatases. Alkaline phosphatase from Escherichia coli reduces myeloperoxidase activity and bacterial translocation. Alkaline phosphatase from Escherichia coli improves survival rate of mice infected with E. coli. Alkaline phosphatase from Escherichia coli improves TNBS-induced colon inflammation.

Orthophosphoric monoester + H₂O → Alcohol + H₃PO₄

Properties

Molecular weight: 80 kDa (Sephadex G–200)

Isoelectric point: pH 4.5

Optimum pH: 9.0　　　　　　　　　　　　　　Figure 1

pH stability: 8.5–10.0 (37°C, 60 min)　　　 Figure 2

Thermal stability: Stable at 45°C and below (pH 9.0, 10 min)　Figure 3

Storage stability: At least one year at −20°C　　　 Figure 4

Activators: Na⁺, Mg²⁺

Applications

This enzyme is useful for enzymatic determination of lecithin when coupled with phospholipase C and choline oxidase.

Assay

■ Principle

The assay is based on the increase in absorbance at 420 nm as p-nitrophenol is liberated according to the following reaction:

■ Unit definition

One unit is defined as the amount of enzyme which liberates 1 μ mole of p-nitrophenol per minute at 37℃ under the conditions specified in the assay procedure.

■ Reagents

1. Reaction mixture

0.5 M Tris–HCl buffer pH 9.0 0.20 ml

10 mM p-nitrophenylphosphate solution　　　　0.20 ml

4 M NaCl solution　　　　　　　　　　　　　 1.00 ml

Distilled water　　　　　　　　　　　　　　 0.50 ml

2. Reaction stopper

0.5 N NaOH solution

3. Enzyme dilution buffer

50 mM Tris–HCl buffer pH 9.0

■ Enzyme solution

Accurately weigh about 20 mg of the sample and add enzyme dilution buffer to make a total of 20 ml. Dilute it with enzyme dilution buffer to adjust the concentration as required.

■ Procedure

1. Pipette accurately 1.90 ml of reaction mixture into a small test tube and preincubate at 37℃.

2. After 5 min, add exactly 100 μl of enzyme solution and mix to start the reaction at 37℃.

※ In the case of a test blank, add 100 μl of enzyme dilution buffer in place of enzyme solution.

3. At 10 minutes after starting the reaction, add 1.0 ml of the reaction stopper to stop the reaction.

4. Measure the absorbance at 420 nm. 　

Absorbance　

sample : As 　　　　　　

blank : Ab 　

△A = (As − Ab) ≦ 0.50 Abs

■ Calculation

14.1 : millimolar extinction coefficient of p-nitrophenol at 420 nm (cm²/μ mole)

10 : reaction time (min)

3.00 : final volume (ml)

0.10 : volume of enzyme solution (ml)

X : concentration of the sample in enzyme solution (mg/ml)

规格

产品名称

碱性磷酸酶 (ALP)

别名

碱性磷酸酯酶 | 碱式磷酸酶 | 碱性磷酸单酯酶 | 正磷酸单酯磷酸水解酶 | 磷酸单酯水解酶（碱性）

英文别名

规格或纯度

生物活性, ActiBioPure™, 天然, 高性能, EnzymoPure™, 来源于大肠杆菌; ≥40 U/mg enzyme powder

生化机理

碱性磷酸酶 (Alkaline phosphatase)，来源于大肠杆菌，是一种主要存在于周质空间的二聚体、非糖基化蛋白质。已知存在三种亚型。这种酶需要锌，并被镁激活。大肠杆菌含皂碱磷酸酶对磷酸酯具有广泛的特异性。碱性磷酸酶是研究磷酸酯酶的模型酶。用于各种生化方法和酶联免疫吸附试验 (ELISA)。

生物活性

≥40 U/mg enzyme powder

种属

大肠杆菌(E.coli)

来源

天然

预测分子量

80 kDa

CAS编号和信息

9001-78-9

酶学委员会编号

EC 3.1.3.1

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

来源于大肠杆菌; ≥40 U/mg enzyme powder

复溶

Reconstitute in 50 mM Tris–HCl buffer pH 9.0 to a concentration of 1mg/ml.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃；复溶后建议分装，避免反复冻融。

单位定义

One unit is defined as the amount of enzyme which liberates 1 μmole of p –nitrophenol per minute at 37℃ under the conditions specified in the assay procedure.

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ26F0939322	分析证书	P755408
ZJ26F0939321	分析证书	P755408

技术文档和文章

p755408 alkaline phosphatase manual.pdf

碱性磷酸酶底物检测系统

目录：技术文章

探索主题:

碱性磷酸酶免疫检测试剂和底物检测酶酶抑制剂和酶底物酶促反应检测

Alkaline Phosphatase Substrate Detection System

目录：技术文章

探索主题:

Alkaline phosphatase Immunoassay reagents and substrates Detection enzymes Enzyme inhibitors and enzyme substrates Enzyme-catalyzed reaction detection

碱性磷酸酶（AP）与辣根过氧化物酶（HRP）显色检测系统全解析：比色/沉淀底物机理、选型与排错（附表1–表3产品导航）

目录：技术文章

探索主题:

HRP 辣根过氧化物酶碱性磷酸酶 AP

Alkaline Phosphatase (AP) and Horseradish Peroxidase (HRP) Colorimetric Detection Systems—A Complete Guide: Mechanisms of Soluble Colorimetric vs. Precipitating Substrates, Selection, and Troubleshooting (with Product Navigator Tables 1–3)

目录：技术文章

探索主题:

HRP Horseradish peroxidase Alkaline phosphatase AP

微球菌核酸酶（MNase）：酶学特性、反应控制与科研及生物制造应用技术综述

目录：技术文章

探索主题:

ChIP MNase 微球菌核酸酶 Ca²⁺依赖 3′-磷酸末端序列偏好染色质核小体 MNase-seq 宿主DNA去除降黏

Micrococcal Nuclease (MNase): A Technical Review of Enzymatic Properties, Reaction Control, and Research and Biomanufacturing Applications

目录：技术文章

探索主题:

MNase micrococcal nuclease Ca2+ dependence 3′-phosphate termini

标记酶在免疫分析、信号放大与多酶检测平台中的功能体系综述

目录：技术文章

探索主题:

多重检测方法学质控标记酶免疫分析信号放大化学发光动力学定量酶级联

Enzyme Labels in Immunoassays, Signal Amplification, and Multi-Enzyme Detection Platforms: A Functional Overview

目录：技术文章

探索主题:

Multiplex detection Methodological Quality Control Enzyme Labels Signal Amplification Immunoassay Chemiluminescence Kinetic Quantification Enzyme Cascades

糖蛋白激素家族的分子特征、信号偏向性与体内动力学

目录：技术文章

探索主题:

糖基化 FSH 糖蛋白激素 LH hCG TSH 共同α亚基特异β亚基信号偏向性体内动力学

Glycoprotein Hormone Family: Molecular Features, Signaling Bias, and In Vivo Pharmacokinetics

目录：技术文章

探索主题:

Glycosylation Pharmacokinetics FSH LH hCG TSH Glycoprotein hormones Common α-subunit Specific β-subunit Signaling bias

pNPP 在 ALP 检测中的定位：定量检测逻辑、适用场景与选用判断

目录：技术文章

探索主题:

pNPP 显色底物 ALP 活性测定

The Positioning of pNPP in ALP Testing: Quantitative Detection Logic, Application Scenarios, and Selection Criteria

目录：技术文章

探索主题:

pNPP ALP activity assays Chromogenic Substrate

大肠杆菌天然生物组分在免疫检测、炎症刺激与对照体系构建中的应用

目录：技术文章

探索主题:

脂多糖碱性磷酸酶大肠杆菌免疫检测天然生物组分炎症刺激脂质A β-葡萄糖醛酸酶对照体系

Applications of Natural Escherichia coli-Derived Biological Components in Immunoassays, Inflammatory Stimulation, and Control System Design

目录：技术文章

探索主题:

Lipopolysaccharide Alkaline phosphatase Escherichia coli Immunoassay Natural biological components Inflammatory stimulation Lipid A β-glucuronidase Control system

二乙醇胺测定法用于碱性磷酸酶的酶法测定实验操作规程

目录：实验方案（Protocols）

探索主题:

DEA 二乙醇胺测定法

Diethanolamine Assay Method for Enzymatic Determination of Alkaline Phosphatase: Experimental Protocol

目录：实验方案（Protocols）

探索主题:

DEA Diethanolamine Assay Method

此产品的引用文献

如何在您的发表作品中引用阿拉丁的产品和网站? 引用文献检索