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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Malate dehydrogenase (MDH) catalyzes the mutual conversion of oxaloacetate and malate, and is associated with the oxidation/reduction of dinucleotide coenzymes.
Reaction

Product description
| Appearance: | White amorphous powder |
| Source: | Microorganism |
| Enzyme Commission Number: | EC 1.1.1.37 |
| CAS Number: | 9001-64-3 |
| Storage temperature: | -20℃ |
| Activity: | ≥50U/mg enzyme powder; ≥200U/mg protein |
| Unit definition: | One unit will convert one micromole of oxaloacetate to L-malate per min at pH 7.5 at 30℃. |
Properties
| Stability: | Stable at -20℃ for at least two years | |
| Molecular weight: | 34 kDa (SDS-PAGE) | |
| Isoelectric point: | 4.9 | |
| Michaelis constant: | 2.5×10⁻⁵M (NADH)2.0×10⁻⁵M (Oxaloacetate) | |
| Optimum pH: | 9.0 | {Fig. 1} |
| Optimum temperature: | 55℃ | {Fig. 3} |
| pH Stability: | 3.5-8.5 (25℃, 20hr) | {Fig. 2} |
| Thermal stability: | < 60℃ (pH7.5, 15min) | {Fig. 4} |
| Inhibitors: | Cu²⁺,Ni²⁺,SDS | |
| Effect of various chemicals: | {Table 1} |
Table 1. Effect of Various Chemicals on MDH
[The enzyme dissolved in 100mM Tris-HCl buffer, pH 7.5 (17U/ml) was incubated with each chemical at 37℃ for 2hr.]

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