重组抗坏血酸氧化酶 (ASO)

CAS: 9029-44-1 货号: R1505819 分子量: Approximately 130 kDa EC号: 232-852-6 PubChem CID: 168009863
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级别和纯度: 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 重组 ? 重组(Recombinant)—— 通过重组表达生产,序列确定、批次稳定。适用于来源明确、无动物源的可重现蛋白。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 245-445 U/mg enzyme powder
Accession #
P24792
生物活性
245-445 U/mg enzyme powder
★
规格
库存
价格
数量
1KU
R1505819-1KU
现货 Stock Image
¥559.90
5KU
R1505819-5KU
现货 Stock Image
¥2,199.90
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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


  Ascorbate oxidase also known as vitamin C oxidase, is a REDOX enzyme involved in the regulation of extracellular matrix. Ascorbate oxidase catalyzes the reaction of ascorbic acid and oxygen to produce dehydroascorbic acid.

Application

  It catalyzes the oxidation of ascorbic acid to produce water and dehydroascorbic acid, and is used for in vitro research.

Assay procedure

Principle

  The disappearance of ascorbic acid is measured at 245nm by spectrophotometry.

Unit definition

  One unit causes the decrease of one micromole of ascorbic acid per minute under the conditions described below.

Reagents

A. Ascorbic acid solution:1.0mM [Dilute the stock solution (10mM) to 10-fold volume with 0.2M KH₂PO₄​ solution containing 1.0mM EDTA.] (Prepare freshly)

Stock solution: 176mg L-ascorbic acid (MW=176.13) / 100 ml of 1.0mM HCl solution containing 1.0mM EDTA (Stable for one month if store at 0~5℃)

B. Na₂HPO₄​​ solution: 10mM

C. HCl solution: 0.2N

D. Enzyme diluent: 10mM Na₂HPO₄​ solution containing 0.05% BSA. (Should be freshly prepared)

Procedure

1. Prepare the following reaction in a test tube and equilibrate at 30℃ for about 5 minutes.

Substrate solution0.5ml(A)
Na₂HPO₄ solution0.5ml(B)

  (pH of the reaction mixture should be 5.6.)

Concentration in assay mixture 

KH₂PO₄82mM
Na₂HPO₄5.5mM
Ascorbic acid0.45mM
EDTA0.45mM
BSA45.4μg/ml

2. Add 0.1ml of the enzyme solution* and mix.

3. After exactly 5 minutes at 30℃, add 3.0ml of HCl solution (C) to stop the reaction and measure the optical density at 245nm against water (OD test).At the same time, prepare the blank by first mixing the reaction mixture with 3.0ml of HCl solution (C) after 5 min-incubation at 30℃, followed by adding the enzyme solution (OD blank).

*Dissolve the enzyme preparation in ice-cold enzyme diluent water (more than 60U/ml) and dilute to 0.15~0.25U/ml with the ice-cold enzyme diluent (D), immediately before assay.

Calculation

Activity can be calculated by using the following formula:

Vt: Total volume (4.1ml)

Vs: Sample volume (0.1ml)

10.0: Millimolar extinction coefficient of ascorbic acid under the assay conditions at pH1.0 (cm²/μmol)

1.0: Light path length (cm)

t: Reaction time (5 minutes)

df: Dilution factor

C: Enzyme concentration in dissolution (C mg/ml)

规格

产品名称
重组抗坏血酸氧化酶 (ASO)
别名
L-抗坏血酸:氧氧化还原酶 | 抗坏血酸酶
英文别名
L-ascorbate oxidase | ascorbase | L-Ascorbate:oxygen oxidoreductase | AAO
规格或纯度
生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,245-445 U/mg enzyme powder
生化机理
May be involved in a redox system involving ascorbic acid.
生物活性
245-445 U/mg enzyme powder
来源
重组表达
CAS编号和信息
9029-44-1
酶学委员会编号
EC 1.10.3.3
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
245-445 U/mg enzyme powder
复溶
Reconstitute in 10mM KH₂PO₄ solution containing 0.05% BSA (Should be freshly prepared).
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃(24个月);复溶后建议分装,避免反复冻融。
单位定义
1个酶活单位等于在pH5.6和30°C条件下1分钟减少1微摩尔抗坏血酸的酶量。

技术文档

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找到2个结果

批号(Lot Number) 证书类型 货号
ZJ26F0130794 分析证书 R1505819
ZJ26F0130793 分析证书 R1505819
技术文档和文章
植物抗坏血酸合成相关糖核苷酸代谢研究进展
Progress in Research on Sugar-Nucleotide Metabolism Related to Ascorbate Biosynthesis in Plants
植物抗坏血酸形成中的半乳糖相关前体转化与氧化还原调控
Galactose-Related Precursor Conversion and Redox Regulation in Plant Ascorbate Biosynthesis
肌酐酶法检测的反应原理、方法体系与结果质量控制
Creatininase-Based Creatinine Detection: Reaction Principles, Method Systems, and Result Quality Control
肌酐含量检测的主要方法、反应原理与结果控制
Creatinine Content Detection: Main Methods, Reaction Principles, and Result Control
酶偶联显色反应中的辅助酶分工:变旋酶、氧化酶、过氧化物酶与脱氢酶
Functional Roles of Auxiliary Enzymes in Enzyme-Coupled Colorimetric Reactions: Mutarotase, Oxidases, Peroxidases, and Dehydrogenases
溶液计算器