己糖激酶 (HK) Accession#: P04806, Accession#: P04807, Amino sugar and nucleotide sugar metabolism, Biosynthesis of secondary metab, Fructose and mannose metabolism, Galactose metabolism, Gene ID: 850614, Gene ID: 852639, Glycolysis / Gluconeogenesis, HK, HXK

Name: 己糖激酶 (HK)
SKU: R1506898
Availability: InStock

己糖激酶 (HK)

CAS: 9001-51-8 货号: R1506898 EC号: 232-611-5 PubChem CID: 176259338

概述规格储存与运输高级数据质检证书(CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器常见问题

概述

Hexokinase can catalyze the phosphorylation of the 6-hydroxyl group of hexose. When coupled with glucose-6-phosphate dehydrogenase, this enzyme can be used for the enzymatic determination of glucose, adenosine 5'-triphosphate (ATP) and creatine kinase.

Application：This product can be used for the rapid clinical detection of α-amylase content in human serum and urine samples, as well as the research and development and production of α-amylase detection kits.

Assay Procedure

PRINCIPLE

The appearance of NADH is measured at 340nm by spectrophotometry.

UNIT DEFINITION

One unit causes the formation of one micromole of NADH per minute at pH8.0 at 30°C.

Reagents:

A. Tris-HCl buffer, pH8.0: 50mM, containing 13.3mM MgCl₂

B. Glucose solution: 0.67M in Tris-HCl buffer solution(A)（The solution should be kept at room temperature at least for 1 hour before use）

C. ATP solution: 16.5mM in Tris-HCl buffer solution(A)（Should be prepared fresh）

D. NAD⁺ solution: 6.8mM in Tris-HCl buffer solution(A)（Should be prepared fresh）

E. G-6-PDH solution: 300U/ml（Dilute with Tris-HCl buffer solution(A)and store on ice）

F. Enzyme diluent: Tris-HCl buffer solution(A) contg.0.1% of bovine serum albumin

Procedure:

1. Prepare the following reaction mixture in a cuvette（d=1.0cm）and equilibrate at 30°C for about 5 minutes.

2.30ml	Tris-HCl buffer solution	（A）
0.50ml	Glucose solution	（B）
0.10ml	ATP solution	（C）
0.10ml	NAD⁺ solution	（D）
0.01ml	G-6-PDH solution	（E）

Concentration in assay mixture

Tris-HCl buffer	50mM
Glucose	0.11M
ATP	0.53mM
NAD⁺	0.22mM
MgCl₂	13mM
BSA	3.2μg/ml
G-6-PDH	ca. 1.0U/ml

2. Add 0.1ml of the enzyme solution* and mix by gentle inversion.

3. Record the increase of optical density at 340nm against water for 4~5minutes in a spectrophotometer thermostated at 30°C, and calculate the △OD per minute from the initial linear portion of the curve (△OD test).

At the same time, measure the blank rate（△OD Blank）by using the same method as the test expect that the enzyme diluent is added instead of the enzyme solution.

*:Dissolve the enzyme preparation in ice-cold enzyme diluent（F）and dilute to 0.1~0.3U/ml with the same buffer, immediately before assay.

Calculation:

Activity can be calculated by using the following formula:

Vt: Total volume（3.11ml）

Vs: Sample volume（0.1ml）

6.22: Millimolar extinction coefficient of NADH under the assay condition (cm²/μM)

1.0: Light path length（cm）

df: Dilution factor

C: Enzyme concentration in dissolution（c mg/ml）

规格

产品名称

己糖激酶 (HK)

英文别名

hexokinase type I | hexokinase type II | hexokinase type III | hexokinase type IV (glucokinase) | HXK1 | HXK2 | HEX1 | HKB | SCI

规格或纯度

生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,≥80%(SDS-PAGE),270-370 U/mg enzyme powder

生物活性

270-370 U/mg enzyme powder

表达系统

E. coli

来源

重组表达

CAS编号和信息

9001-51-8

酶学委员会编号

EC 2.7.1.1

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

270-370 U/mg enzyme powder

复溶

Reconstitute in ice-cold 50mM Tris-HCl buffer, pH8.0, containing 13.3mM MgCl2 and 0.1% of bovine serum albuminand, dilute to 0.1~0.3U/ml with the same buffer, immediately before assay.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（36个月）；复溶后建议分装，避免反复冻融；避光。

单位定义

One unit causes the formation of one micromole of NADH per minute at pH8.0 at 30°C.

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ26F0333378	分析证书	R1506898
ZJ26F0333377	分析证书	R1506898

技术文档和文章

从蔗糖到单糖：糖转化过程中的关键酶节点与调控逻辑

目录：技术文章

探索主题:

蔗糖酶催化葡萄糖糖代谢单糖转化酶蔗糖合成酶蔗糖磷酸化酶果糖碳流分配

From Sucrose to Monosaccharides: Key Enzymatic Nodes and Regulatory Logic in Sugar Conversion

目录：技术文章

探索主题:

sucrose Glucose monosaccharides invertase sucrose synthase sucrose phosphorylase

糖酵解限速酶调控：己糖激酶、磷酸果糖激酶与丙酮酸激酶的功能比较

目录：技术文章

探索主题:

己糖激酶代谢重编程 PKM2 糖酵解限速酶磷酸果糖激酶丙酮酸激酶糖酵解通量肿瘤代谢

Regulation of Glycolytic Rate-Limiting Enzymes: Functional Comparison of Hexokinase, Phosphofructokinase, and Pyruvate Kinase

目录：技术文章

探索主题:

Hexokinase Metabolic Reprogramming PKM2 Glycolytic Rate-Limiting Enzymes Phosphofructokinase Pyruvate Kinase Glycolytic Flux Tumor Metabolism

葡萄糖酶法检测技术比较：葡萄糖氧化酶、葡萄糖脱氢酶与己糖激酶法的应用选择

目录：技术文章

探索主题:

NADPH 比色法葡萄糖检测葡萄糖氧化酶法葡萄糖脱氢酶法己糖激酶法酶偶联反应血糖检测

Comparison of Enzymatic Glucose Detection Methods: Glucose Oxidase, Glucose Dehydrogenase, and Hexokinase Methods

目录：技术文章

探索主题:

NADPH Colorimetric Method Glucose Detection Glucose Oxidase Method Glucose Dehydrogenase Method Hexokinase Method Enzyme-Coupled Reaction Blood Glucose Detection

葡萄糖-6-磷酸（G6P）在糖代谢分流、NADPH生成与疾病机制研究中的应用

目录：技术文章

探索主题:

葡萄糖-6-磷酸 G6P

Applications of Glucose-6-Phosphate (G6P) in Glycolytic Branching, NADPH Generation, and Disease Mechanism Research

目录：技术文章

探索主题:

G6P glucose-6-phosphate

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

常见问题

本产品的纯度是多少？如何测定？

本产品的纯度为 ≥80%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。

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己糖激酶 (HK)

概述

规格

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

常见问题

需要帮助选择级别？

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