Recombinant Mouse IL-2 Protein

货号: rp154170
有货
级别和纯度: 无动物源 ? 无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 ≥95%(SDS-PAGE) 见COA
Accession #
P04351
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<0.1 EU/μg
生物活性
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 0.88-3.5 ng/mL, corresponding to a specific activity of 2.86×10^5-1.14×10^6 units/mg.
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规格
库存
价格
数量
10μg
rp154170-10μg
现货 Stock Image
¥529.90
50μg
rp154170-50μg
现货 Stock Image
¥1,999.90
100μg
rp154170-100μg
现货 Stock Image
¥2,799.90
1mg
rp154170-1mg
期货 Stock Image
¥11,699.90
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规格

产品名称
Recombinant Mouse IL-2 Protein
别名
重组小鼠IL-2蛋白 | 重组小鼠白细胞介素2蛋白
英文别名
Aldesleukin | IL2 | IL-2 | IL-2lymphokine | interleukin 2 | interleukin-2 | involved in regulation of T-cell clonal expansion | Proleukin | T cell growth factor | T-cell growth factor | TCGF
规格或纯度
无动物源, 无载体, 生物活性, ActiBioPure™, 高性能, His标签, ≥95%(SDS-PAGE), 见COA
生化机理
Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance.Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
生物活性
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 0.88-3.5 ng/mL, corresponding to a specific activity of 2.86×10^5-1.14×10^6 units/mg.
表达系统
HEK293
种属
小鼠(Mouse)
氨基酸
21-169 aa
序列
APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQHHHHHH
蛋白标签
C-His
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
18 kDa
SDS-PAGE
20 kDa,under reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 8% Trehalose,5% Mannitol, pH 7.4
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Mouse IL-2 Protein (rp154170) - Protein Bioactivity 
Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 2.4 ng/mL.
Recombinant Mouse IL-2 Protein (rp154170) - SDS-PAGE 
2 μg/lane of Recombinant Mouse IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 20 - 25 kDa.

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批号(Lot Number) 证书类型 货号
ZJ26F0131813ZJ26F0131813 分析证书 rp154170
ZJ26F0131812ZJ26F0131812 分析证书 rp154170
ZJ26F0131811ZJ26F0131811 分析证书 rp154170
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