Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 0.88-3.5 ng/mL, corresponding to a specific activity of 2.86×10^5-1.14×10^6 units/mg.
Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance.Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
生物活性
Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 0.88-3.5 ng/mL, corresponding to a specific activity of 2.86×10^5-1.14×10^6 units/mg.
Store at -20°C for 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
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Recombinant Mouse IL-2 Protein (rp154170) - Protein Bioactivity
Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 2.4 ng/mL.
Recombinant Mouse IL-2 Protein (rp154170) - SDS-PAGE
2 μg/lane of Recombinant Mouse IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 20 - 25 kDa.