Recombinant Mouse IFN-gamma Protein

货号: rp154254
有货
级别和纯度: 无动物源 ? 无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 高性能 ? 高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 ≥95%(SDS-PAGE)
Accession #
P01580
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<0.1 EU/μg
生物活性
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL.
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规格
库存
价格
数量
10μg
rp154254-10μg
现货 Stock Image
¥199.90
50μg
rp154254-50μg
现货 Stock Image
¥499.90
100μg
rp154254-100μg
现货 Stock Image
¥799.90
1mg
rp154254-1mg
期货 Stock Image
¥4,999.90
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为什么选择此级别

ActiBioPure™,His标签,无动物源,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Mouse IFN-gamma Protein
别名
重组小鼠IFN-gamma蛋白 | 重组小鼠干扰素γ蛋白
英文别名
IFG | IFI | IFNG | IFNgamma | IFN-gamma | Immune interferon | interferon gamma | interferon, gamma | IFN gamma | IFNγ | If2f | IFN-g
规格或纯度
无动物源, 无载体, 生物活性, ActiBioPure™, 高性能, His标签, ≥95%(SDS-PAGE)
生化机理
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
生物活性
Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL.
表达系统
HEK293
种属
小鼠(Mouse)
氨基酸
23-155 aa
序列
HGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRCHHHHHH
蛋白标签
C-His
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
16.3 kDa
SDS-PAGE
18-21 kDa, under reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
发货时温度为 4°C。储存温度为 -20°C。成分:0.15% 氯化钠、0.16% 磷酸钠 本品为活性蛋白质,可能会引起体内生物反应,请谨慎处理。
图片

Recombinant Mouse IFN-gamma Protein (rp154254) - Protein Bioactivity
Measured by its ability to inhibit the proliferation of HT-29 human colon cancer cells. The ED₅₀ for this effect is 4.8 ng/mL.

Recombinant Mouse IFN-gamma Protein (rp154254) - SDS-PAGE
2 μg/lane of Recombinant Mouse IFN-gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. As a result of different glycosylation, it migrates as approximately 15 & 18 kDa band in SDS-PAGE under reducing conditions and 15 & 18 & 32 & 36 kDa under non-reducing condition.

应用
应用名称
稀释比例
WB

1/4000

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

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高级数据

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找到6个结果

批号(Lot Number) 证书类型 货号
ZJ26F0838720ZJ26F0838720 分析证书 rp154254
ZJ26F0838719ZJ26F0838719 分析证书 rp154254
ZJ26F0838718ZJ26F0838718 分析证书 rp154254
ZJ25F0927483ZJ25F0927483 分析证书 rp154254
ZJ25F0927484ZJ25F0927484 分析证书 rp154254
ZJ25F0927485ZJ25F0927485 分析证书 rp154254
技术文档和文章
溶液计算器