无动物源,无载体,生物活性,ActiBioPure™,高性能,His标签,≥95%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Transforming growth factor beta-2 proprotein. Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.Latency-associated peptide. Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2. Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps : following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.Post-translational: Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.
Post-translational: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-2 (TGF-beta-2) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-2 inactive.
生物活性
Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 2.49 ng/mL.
12.5 & 40.7-46.8 & 51.5-58.1 kDa, under reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 5% Mannitol, 5% Trehalose, pH 7.4.
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human TGF-beta 2 Protein (rp181279) - Protein Bioactivity
Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 2.49 ng/mL.
Recombinant Human TGF-beta 2 Protein (rp181279) - SDS-PAGE
3 μg/lane of Recombinant Human TGF-beta 2 Protein was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing the bands at 12.5 & 40.7-46.8 & 51.5-58.1 kDa.