级别和纯度: 无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。MBP标签?麦芽糖结合蛋白(MBP) 标签级 —— 带有 MBP 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。MBP 还能改善溶解性。≥95%(SDS-PAGE)expressed in E. coli, 见COA
Accession #
P18340
蛋白标签
N-MBP & His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Determined by its ability to chemoattract human activated T cells cultured in the presence of IL-2 (rp228980) using a concentration range of 50.0-150.0 ng/mL.
无载体, 生物活性, ActiBioPure™, 高性能, His标签, MBP标签, ≥95%(SDS-PAGE), expressed in E. coli, 见COA
生化机理
CXCL9, also known as MIG, is a member of the alpha subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
生物活性
Determined by its ability to chemoattract human activated T cells cultured in the presence of IL-2 (rp228980) using a concentration range of 50.0-150.0 ng/mL.
54.0 kDa, under reducing conditions; 55.5 & 120.7 & 250.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
expressed in E. coli, 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
图片
Recombinant Mouse CXCL9/MIG Protein (rp183583) - Protein Bioactivity
Determined by its ability to chemoattract human activated T cells cultured in the presence of IL-2 (rp228980) using a concentration range of 50.0 - 150.0 ng/mL.
Recombinant Mouse CXCL9/MIG Protein (rp183583) - SDS-PAGE
2 μg/lane of Recombinant Mouse CXCL9/MIG Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 54.0 kDa under reducing conditions and 55.5 & 120.7 & 250.0 kDa under non-reducing conditions.
This protein polymer formation is linked by disulfide bonds (Uniport: P18340).