The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.
生物活性
Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions.
30.5 kDa, under reducing conditions; 30.5 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris, 150mM NaCl, pH 7.5
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Carbonic Anhydrase XIII Protein (rp183661) - SDS-PAGE 2 μg/lane of Recombinant Human Carbonic Anhydrase XIII Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.5 kDa.
Recombinant Human Carbonic Anhydrase XIII Protein- Enzyme Activity Measured by its esterase activity. The specific activity is >5 pmol/min/µg, as measured under the described conditions. Conditions: Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5 Recombinant Human Carbonic Anhydrase XIII Protein (rp183661) Substrate: 4-Nitrophenyl Acetate (4-NPA) (N111060), 100 mM stock in acetone 1.Dilute rhCA13 to 100 ng/µL in Assay Buffer. 2.Dilute Substrate to 2.0 mM in Assay Buffer. 3.In a plate load 50 µL of 100 ng/µL rhCA13, and include a Substrate Blank containing 50 µL Assay Buffer. 4.Start the reaction by adding 50 µL of 2.0 mM Substrate to wells containing rhCA13 and Assay Buffer. 5.Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
ZJ24F1216158
危险品化学品经营许可证（带存储）