Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK. It has no predicted N-glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature mouse DMC molecule of 97 aa and 11 kDa size. DMC is constitutively produced by airway and intestinal epithelium. It induces the chemotaxis of quiescent, but not LPS‑activated peripheral blood monocytes and dendritic cells, and also binds these cells specifically (1). DMC expression is increased in endothelial cells when they are induced to form tubes in vitro. Transgenic overexpression in NIH3T3 cells causes up‑regulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity. DMC and two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show significantly correlated expression with that of VEGF in primary lung, breast and esophageal tumors. DMC is therefore suggested to play a role in tumor angiogenesis. Mature mouse DMC shares 82%, 71% and 66% amino acid sequence identity with rat, human and bovine DMC, respectively.
Post-translational: Likely to undergo an endoproteolytic process to form a four-cysteine-containing mature peptide with a canonical CXC chemokine scaffold after secretion.
10.1-18.1 kDa, under reducing conditions; 10.0-18.6 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Mouse CXCL17/VCC-1 Protein (rp184867) - SDS-PAGE 2 μg/lane of Recombinant Mouse CXCL17/VCC-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.1-18.1 kDa under reducing conditions and 10.0-18.6 & 26.0-30.8 kDa under non-reducing conditions. The observation of CXCL17/VCC-1 Protein as both dimers and monomers under non-reducing (NR) conditions suggests that a portion of the protein forms dimers. This dimerization may be mediated by (or stabilized by) disulfide bonds (Uniprot: Q5UW37).
ZJ26F0636316
危险品化学品经营许可证（带存储）