无载体,生物活性,ActiBioPure™,His标签,SUMO标签,≥85%(SDS-PAGE),expressed in E. coli; 见COA
生化机理
Double minute 2 protein (Mdm2, also known as Hdm2) is a RING-finger Ubiquitin E3 ligase that acts as a major regulator of the tumor suppressor p53. Mdm2 inhibits p53-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. The E3 ligase activity is confined to the C-terminal domain of Mdm2 and is responsible for the ubiquitination and subsequent proteasomal degradation of p53. Although the isolated RING domain is capable of p53 ubiquitination, other regions of the protein including a central acidic domain are also crucial for full E3 ligase activity. Mdm2 also regulates its own intracellular levels by auto-ubiquitination, and can be SUMOylated which decreases autoubiquitination activity but increases activity toward p53. Mdm2 also affects the cell cycle and apoptosis through interactions with other proteins such as retinoblastoma1 (pRB) and ribosomal protein L5.
Post-translational: Phosphorylation on Ser-166 by SGK1 activates ubiquitination of p53/TP53 (PubMed:19756449). Phosphorylated at multiple sites near the RING domain by ATM upon DNA damage; this promotes its ubiquitination and degradation, preventing p53/TP53 degradation (PubMed:10611322, PubMed:12167711, PubMed:18382127, PubMed:19816404, PubMed:26124108). Autoubiquitination leads to proteasomal degradation; resulting in p53/TP53 activation it may be regulated by SFN (PubMed:18382127, PubMed:30879903).
Also ubiquitinated by TRIM13 (PubMed:21333377).
ATM-phosphorylated MDM2 is ubiquitinated by the SCF(FBXO31) complex in response to genotoxic stress, promoting its degradation and p53/TP53-mediated DNA damage response (PubMed:26124108).
Deubiquitinated by USP2 leads to its accumulation and increases deubiquitination and degradation of p53/TP53 (PubMed:17290220).
Deubiquitinated by USP7 leading to its stabilization (PubMed:15053880).
生物活性
Immobilized Recombinant Human MDM2 Protein (rp184920) at 2.0 μg/mL can bind MDM2 Antibody (Ab114718) with the EC50 of 65.6 ng/mL.
Immobilized Recombinant Human MDM2 Protein (rp184920) at 2.0 μg/mL can bind MDM2 Antibody (Ab114718) with the EC₅₀ of 65.6 ng/mL.
Recombinant Human MDM2 Protein (rp184920) - SDS-PAGE
2 μg/lane of Recombinant Human MDM2 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 107.4 kDa under reducing conditions.
ZJ26F0535545
危险品化学品经营许可证（带存储）