(Small) Mothers Against Decapentaplegic | Deleted in Pancreatic Carcinoma | Deleted in Pancreatic Carcinoma 4 | Deleted in pancreatic carcinoma locus 4 | Deletion target in pancreatic carcinoma 4 | DPC 4 | DPC4 | hSMAD4 | JIP | MAD homolog 4 | MAD mothers
规格或纯度
无载体,His标签,≥90%(SDS-PAGE),见COA
生化机理
Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor.
Post-translational: Phosphorylated by PDPK1. Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human SMAD4 Protein (rp214404) - SDS-PAGE 3 μg/lane of Recombinant Human SMAD4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 62.1 kDa under reducing conditions and 58.4 kDa under non-reducing conditions.
ZJ25F0521146
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