1、 Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. 2、Measured in a cell proliferation assay using K562 human chronic myelogenous leukemia erythroleukemic cells. The ED50 for this effect is typically 21.02 ng/mL.
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Application: It can be used in animal cell culture. Matters need attention: This product is for scientific research, laboratory and production use only, and is not intended for clinical treatment. It should not be used for animals and humans.
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
生物活性
1、 Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. 2、Measured in a cell proliferation assay using K562 human chronic myelogenous leukemia erythroleukemic cells. The ED50 for this effect is typically 21.02 ng/mL.
Lyophilized from a 0.2 um filtered solution in sterile water.
浓度
铁含量(Iron content)>1000 ppm
复溶
It is recommended to redissolve in sterile deionized water.
储存条件
2-8°C储存
运输条件
低温运输
稳定性与储存
36 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ to -80℃ under sterile conditions after reconstitution. 7-10 days at 2℃ to 8℃ under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human Holo-Transferrin (rp218450) - Protein Bioactivity Measured in a cell proliferation assay using K562 human chronic myelogenous leukemia erythroleukemic cells. The ED50 for this effect is typically 21.02 ng/mL.
Recombinant Human Holo-Transferrin (rp218450) - SDS-PAGE 2 μg/lane of Recombinant Human Holo-Transferrin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 75 kDa under reducing conditions and 70 kDa under non-reducing conditions.