计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
皂化液主要由乙醇、强碱等组成。皂化属于化学修饰和阻断法的一种,其原理是利用含酒精的碱性溶液裂解耐酶唾液酸等中的O-乙酰基,O-乙酰基的去除及唾液酸侧链羟基的形成恢复了唾液酸对PAS的反应性,皂化技术也被用于逆转甲基化,即裂解甲基化过程中形成的甲基酯键,重新形成羧基。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考)
按实验具体要求操作,一般室温处理30min。
注意事项
1、需要阳性对照片以便验证甲基化程序的有效性。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用,以防影响后续实验效果。
Saponification solution is primarily composed of ethanol and strong alkali. Saponification is a type of chemical modification and blocking method. Its principle involves using an alcohol-containing alkaline solution to cleave O-acetyl groups in enzyme-resistant sialic acids. The removal of O-acetyl groups and the formation of hydroxyl groups on the sialic acid side chains restore the reactivity of sialic acids to PAS. Saponification technology is also used to reverse methylation by cleaving the methyl ester bonds formed during the methylation process and reforming carboxyl groups. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
Procedure (for reference only)
Operate according to the specific experimental requirements. Typically, treat at room temperature for 30 minutes.
Precautions
Positive control slides are required to verify the effectiveness of the methylation procedure.
For your safety and health, please wear a lab coat and disposable gloves during operation.
After opening, the reagent should be used as soon as possible to avoid affecting subsequent experimental results.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | S1510327 |