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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
土壤β-葡萄糖苷酶(S-β-GC)能够催化水解芳基或烃基与糖基原子团之间的糖苷键生成葡萄糖,是纤维素分解酶系中重要组成成分之一,在土壤微生物的糖类代谢方面具有重要生理功能。本试剂盒提供了一种简单、方便、快速的 S-β-GC 活性检测方法,适用于土壤样本。其原理是 S-β-GC 能够催化对-硝基苯-β-D-吡喃葡萄糖苷生成对-硝基苯酚,后者在 400 nm 有特征光吸收。
Soil β-glucosidase (S-β-GC) catalyzes the hydrolysis of glycosidic bonds between aryl or alkyl groups and glycone moieties to release glucose. It is a vital component of the cellulose-degrading enzyme system and plays a significant physiological role in the carbohydrate metabolism of soil microorganisms. This kit provides a simple, convenient, and rapid method for determining S-β-GC activity, suitable for soil samples. The principle is based on S-β-GC catalyzing the hydrolysis of p-nitrophenyl-β-D-glucopyranoside to generate p-nitrophenol, which exhibits characteristic light absorption at 400 nm.