氨基磁珠 (500 nm) 以超顺磁性的Fe3O4 为内核，表面修饰氨基功能团，具有高磁含量，单分散性等特点。丰富的氨基功能团能够在特定的试剂作用下与蛋白等生物配体共价结合到磁珠表面，从而实现快速地将目标物从样品分离与富集，是分子生物学、医学等相关领域研究的重要载体。
产品特点
1. 磁吸响应快，单分散性好， 磁珠粒径分布：CV<5%
2. 批间差异小，重复性高
3. 生物相容性好，细胞毒性小
4.方便与多种生物分子偶联，操作简单
适用范围
1. 靶向药物
2. 生物活性物质检测
3. 样品富集与分离
与生物分子偶联方法（戊二醛法）
1.磁珠表面氨基活化
将磁珠混匀后，取适量磁珠至离心管中，用20 mM pH 7.4 PBS 缓冲液洗涤两次，磁吸弃上清。加入新配置的15%戊二醛溶液，混匀磁珠，于25℃避光反应1 小时（反应时，保持磁珠悬浮）。
2.偶联生物分子
磁珠活化后，以20 mM pH 7.4 PBS 缓冲液洗涤两次，磁吸去上清，加入适量的生物分子，用pH为8.0 的缓冲液分散磁珠(必要时可加入0.05% Tween 20 保持磁珠分散)，于25℃避光反应3 小时或4℃避光反应过夜（注意：偶联时确保磁珠均匀悬浮）。
3.封闭
磁吸偶联后的磁珠，弃去上清，加入适量含有5% BSA 的PBS 缓冲液（20 mM, pH 7.4）重悬磁珠，25℃避光反应1 小时，以封闭未反应的活性位点，之后以PBS 缓冲液（20 mM, pH 7.4）分散磁珠，于4℃保存，必要时可加入0.02%叠氮钠抑制细菌生长。
注意事项
1. 磁珠取用前应充分混匀，防止取用改变磁珠浓度，避免长时间超声对磁珠表面破坏；
2. 磁珠取出后，使用前请进行磁分离并用纯水或所用缓冲溶液清洗2-3遍；
3. 磁珠使用和保存过程中应避免冻融或烘干。
Amine Magnetic Beads (500 nm) featuring superparamagnetic Fe₃O₄ as the core and a surface modified with amine (–NH₂) functional groups, this product exhibits high magnetic content and excellent monodispersity. The abundant amine groups enable covalent binding of proteins and other biological ligands to the bead surface via specific crosslinkers, allowing rapid separation and enrichment of target molecules from complex samples. It serves as an important carrier in molecular biology, medical research, and related fields.
Product Features
1.Fast magnetic response and excellent monodispersity; bead size distribution: CV < 5%.
2.Low batch‑to‑batch variation and high reproducibility.
3.Good biocompatibility and low cytotoxicity.
4.Convenient coupling to various biomolecules with simple operation.
Applications
1.Targeted drug delivery.
2.Detection of bioactive substances.
3.Sample enrichment and separation.
Coupling Protocol for Biomolecules (Glutaraldehyde Method)
Step 1: Activation of amine groups on the bead surface
After thorough resuspension, transfer an appropriate volume of magnetic beads to a centrifuge tube. Wash twice with 20 mM PBS buffer (pH 7.4), magnetic separate, and discard the supernatant. Add freshly prepared 15% glutaraldehyde solution, resuspend the beads, and incubate at 25°C for 1 hour in the dark (keep the beads suspended during the reaction).
Step 2: Coupling of biomolecules
After activation, wash the beads twice with 20 mM PBS buffer (pH 7.4), magnetic separate, and remove the supernatant. Add the desired biomolecule and disperse the beads in a buffer at pH 8.0 (0.05% Tween 20 may be added if necessary to maintain dispersion). Incubate at 25°C for 3 hours or at 4°C overnight in the dark, ensuring that the beads remain uniformly suspended during coupling.
Step 3: Blocking
Magnetic separate the coupled beads and discard the supernatant. Resuspend the beads in PBS buffer (20 mM, pH 7.4) containing 5% BSA. Incubate at 25°C for 1 hour in the dark to block unreacted active sites. After blocking, disperse the beads in PBS buffer (20 mM, pH 7.4) and store at 4°C. If necessary, add 0.02% sodium azide to inhibit bacterial growth.
Precautions
1.Thoroughly resuspend the beads before each use to avoid changes in bead concentration during sampling. Avoid prolonged sonication to prevent damage to the bead surface.
2.After removal from the container and before use, perform magnetic separation and wash the beads 2–3 times with purified water or the desired buffer solution.
3.Avoid freeze‑thaw cycles or drying of the magnetic beads during use and storage.
ZJ26F0838642
危险品化学品经营许可证（带存储）