计算溶液所需的质量、体积或浓度。
BioReagent,即用型,无菌,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胰蛋白酶(Trypsin)是一种丝氨酸水解酶,它能把多肽链中赖氨酸和精氨酸残基中的羧基侧切段,水解细胞间的蛋白质,破坏细胞间的连接,从而使组织或贴壁细胞离散成单个细胞。胰酶分散细胞的活性与组织或细胞的特性、胰酶浓度、温度和作用时间有关,在PH 8.0和37℃时,胰酶的作用能力最强,因此使用胰酶时,应把握好浓度、温度和时间,以免消化过度造成细胞损伤。
一般常用胰酶的工作浓度为0.25%,而半贴壁细胞或对胰酶敏感的细胞常采用低浓度(0.05%)的胰酶进行细胞消化。EDTA能够螯合Ca²⁺和Mg²⁺,从而破坏细胞连接促进细胞的解离,因此在胰酶溶液中常常会加入一定量的EDTA混合使用,以增强解离效果。
产品为即用型,已经过滤除菌,可直接使用,使用前无需稀释或过滤除菌等任何处理,使用时应注意无菌操作,避免污染。
产品参数:
| 指标 | 参数 |
| 外观 | 液体 |
| 溶剂 | 10 mM PBS |
| 胰蛋白酶浓度 | 0.5 g/L |
| EDTA•2Na | 0.2 g/L |
| 酚红指示剂 | 0.2 g/L |
注意和声明:
如有以下情况,请勿使用:
1. 包装有破损。
2. 使用时已超出规定有效期。
3. 仅用于研究和进一步生产,不得直接用于人或动物。
Trypsin is a serine hydrolase. It cleaves peptide bonds at the carboxyl sides of lysine and arginine residues in polypeptide chains, hydrolyzes intercellular proteins and breaks down intercellular junctions, so as to disperse tissues or adherent cells into single cells. The cell dissociation activity of trypsin is affected by cell/tissue properties, trypsin concentration, temperature and incubation time. Trypsin exhibits the highest activity at pH 8.0 and 37 °C. Appropriate concentration, temperature and incubation time must be strictly controlled to avoid cell damage caused by over-digestion.
The commonly used working concentration of trypsin is 0.25%. Low-concentration trypsin (0.05%) is usually used for semi-adherent cells and trypsin-sensitive cells. EDTA can chelate Ca²⁺ and Mg²⁺ to disrupt cell adhesion and facilitate cell dissociation. Therefore, an appropriate amount of EDTA is often added into trypsin solution to improve dissociation efficiency.
This ready-to-use product has been sterile-filtered for direct application. No dilution, filtration or other pretreatment is required before use. Aseptic operation must be performed to prevent contamination.
Product Specifications:
| Index | Parameter |
| Appearance | Liquid |
| Solvent | 10 mM PBS |
| Trypsin Concentration | 0.5 g/L |
| EDTA•2Na | 0.2 g/L |
| Phenol Red Indicator | 0.2 g/L |
Notes & Statements:
Do not use this product under the following conditions:
1. Damaged packaging.
2. Expired shelf life.
3. For research and further production use only; not for direct application to humans or animals.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | T1523187 |
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