淀粉,是一种碳水化合物的高度聚合体,它是由两个多聚糖,即直链淀粉和支链淀粉组成的混合物。淀粉需要稳定的耐高温淀粉酶进行作用才能产生较短链的糊精。葡萄淀粉酶则主要用于葡萄糖浆、高果糖浆和用于酒精生产业的淀粉的水解。本产品是由黑曲霉(Aspergillus niger)优良菌种经深层发酵精制提炼而成。广泛应用于酿酒、淀粉加工、淀粉糖、酒精、味精和抗生素等行业。
工作机理:
糖化酶又称葡萄糖淀粉酶,能从淀粉分子的非还原性末端开始,水解α -1,4糖苷键,从而产生6-C和-D-葡萄糖。这种酶也能水解α-1,6-葡萄糖苷键。
产品特性:
本产品的pH范围是: 4.0-4.5,适用的pH范围是3.0-5.5,本产品温度范围是40-60℃(104-140°F), 最佳温度范围是: 58-60℃ (136-140°F)。
产品规格:
1.酶活力≥100,000 U/ml
2.容重≤1.25g/ml;
3.PH 3.0—5.0
酶活力定义:
1g固体酶(或1ml液体酶)在 40℃、 pH4.6条件下,1 小时内水解可溶性淀粉产生1mg葡萄糖所需要的酶量,即为 1 个酶活力单位。
执行标准:
执行中华人民共和国行业标准QB1805.2-93
使用方法:
本产品用于酿酒酵母、淀粉、柠檬酸等的制造行业。该酶添加量为100-300U/g(pH4.0-5.0,温度60℃)
1. 酒精工业(淀粉质为原料):醪液经蒸煮冷却至59±1℃时,加入糖化酶,拌均匀,保温30分钟,冷却后送入发酵。建议加酶用量为120-150U/g原料。(如果原料质量差,有霉烂变质时加酶量应提高到150-180U/g原料)。
2. 白酒工业:固体酒培经蒸煮冷却至要求温度范围时,将糖化酶按要求量加入加浆水中,均匀加入酒培中(也可与大曲、酒母并用)。推荐使用量:250-300U/g原料。(注:糖化酶在白酒生产中应考虑发酵周期的长短而确定使用量。)
3. 淀粉糖工业:添加比例为 100-300 U/g(pH为4.2-4.5)。混合物温度保持在60℃。
4. 啤酒工业:在糖化作用或者发酵以前添加。
5. 酒水酿造和醋制造业:添加带有酶的发酵酵母能够提高产率。
注意事项:
本产品无毒,可生物降解。避免不必要接触,长期接触一些产品中的蛋白质会使有的人对该产品敏感。每次接触产品后要用温水、香皂洗手,将产品放在儿童不能触及的地方。
Starch is a highly polymerized carbohydrate and a mixture composed of two polysaccharides: amylose and amylopectin. Starch requires stable, thermostable α-amylase to break down into short-chain dextrins. Glucoamylase is mainly used for the hydrolysis of starch in the production of glucose syrup, high-fructose corn syrup, and industrial alcohol.
This product is refined and produced via submerged fermentation using excellent strains of Aspergillus niger. It is widely applied in industries including brewing, starch processing, starch sugar, alcohol, monosodium glutamate, and antibiotics.
Mechanism of Action
Glucoamylase, also known as glucan 1,4-α-glucosidase, hydrolyzes α-1,4 glycosidic bonds starting from the non-reducing ends of starch molecules, yielding D-glucose. This enzyme can also hydrolyze α-1,6 glucosidic linkages.
Product Characteristics
Optimum pH range: 4.0–4.5
Effective pH range: 3.0–5.5
Effective temperature range: 40–60 °C (104–140 °F)
Optimum temperature range: 58–60 °C (136–140 °F)
Product Specifications
Enzyme activity ≥ 100,000 U/mL
Bulk density ≤ 1.25 g/mL
pH: 3.0–5.0
Enzyme Activity Definition
One unit of enzyme activity (U) is defined as the amount of enzyme that hydrolyzes soluble starch to produce 1 mg of glucose in 1 hour at 40 °C and pH 4.6, per gram of solid enzyme or per milliliter of liquid enzyme.
Implemented Standard
This product complies with QB/T 1805.2‑93, the industrial standard of the People’s Republic of China.
Application Methods
This product is used in the manufacturing of active dry yeast, starch, citric acid, etc. The recommended dosage is 100–300 U/g (at pH 4.0–5.0, 60 °C).
1. Alcohol industry (starch-based raw materials)
After cooking, cool the mash to 59±1 °C, add glucoamylase, mix evenly, and hold for 30 minutes. Cool before fermentation.
Recommended dosage: 120–150 U/g raw material.
If raw materials are of poor quality or moldy, increase dosage to 150–180 U/g.
2. Chinese liquor industry
Cool the cooked solid starter to the target temperature. Dissolve glucoamylase in water and mix evenly into the starter (may be used together with Daqu and yeast).
Recommended dosage: 250–300 U/g raw material.
Note: Dosage should be adjusted according to fermentation cycle length.
3. Starch sugar industry
Dosage: 100–300 U/g at pH 4.2–4.5. Maintain temperature at 60 °C.
4. Beer industry
Add prior to saccharification or fermentation.
5. Wine and vinegar production
Using yeast supplemented with this enzyme can improve fermentation yield.
Precautions
This product is non-toxic and biodegradable. Avoid unnecessary contact. Prolonged exposure to proteins in some formulations may cause sensitization in certain individuals. Wash hands with warm water and soap after handling. Keep out of reach of children.