基本信息

产品名称	Recombinant CD4 Antibody (PE)
别名	抗人CD4单克隆抗体 \| Anti-CD4抗体 \| CD4抗体
英文别名	CD_antigen: CD4 \| CD4 antigen (p55) \| CD4 antigen \| CD4 molecule \| CD4 receptor \| CD4 \| CD4mut \| T-cell surface antigen T4/Leu-3 \| T-cell surface glycoprotein CD4 \| CD 4 antibody \| CD4 (L3T4) antibody \| CD4 antibody \| CD4 antigen (p55) antibody \| CD4 anti
规格或纯度	ExactAb™, 已验证, 5 μL/test
生化机理	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:12089508, PubMed:16331979, PubMed:2214026, PubMed:9641677). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).
宿主种属	小鼠(Mouse)
特异性	Human CD4
种属反应性	人(Human)
免疫原	Human peripheral T cells
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes
偶联	PE
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	575nm
Ex/Em(nm)	Ex:565nm;Em:575nm

AI解读

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冻结
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

图片

Flow Cytometry: Recombinant CD4 Antibody (PE) (Ab213637)

Recombinant CD4 Antibody (PE) (Ab213637) - Flow Cytometry
Flow cytometry analysis of CD4 expression by human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD4 Antibody (PE) (Ab213637) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE) (Ab180308).

Flow Cytometry: Recombinant CD4 Antibody (PE) (Ab213637)

Recombinant CD4 Antibody (PE) (Ab213637) - Flow Cytometry
Flow cytometry analysis of CD4 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD4 Antibody (PE) (Ab213637) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

关联靶点（人）

CD4 Tclin T 细胞表面糖蛋白 CD4(T-cell surface glycoprotein CD4) (0 活性数据)

点击查看靶蛋白信息： CD4

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

技术规格说明书

应用

应用名称	稀释比例
Flow Cytometry	5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.