计算溶液所需的质量、体积或浓度。
瑞氏染色试剂盒
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
瑞氏色素是酸性染料伊红 (Eosin) 和碱性染料亚甲蓝 (Methylene Blue) 组成的复合染料，对原生质的染色有很好的区别作用。各种细胞成分化学性质不同，对各种染料的亲和力也不一样，如血红蛋白、嗜酸性颗粒为碱性蛋白质，与酸性染料伊红结合，染粉红色，称为嗜酸性物质；细胞核蛋白、淋巴细胞、嗜碱性粒细胞胞质为酸性，与碱性染料结合后，染紫蓝色或蓝色，称为嗜碱性物质；中性颗粒呈等电状态与伊红和美蓝均可结合，染淡紫红色，称为嗜中性物质。原始红细胞、早幼红细胞胞质、核仁含较多酸性物质，染成较浓厚的蓝色；中幼红细胞既含酸性物质，又含碱性物质，染成红蓝色或灰红色；完全成熟红细胞，酸性物质彻底消失后，染成粉红色。瑞氏染液为蓝色澄明液体，有甲醇特异香味，主要由美蓝、伊红、甲醇等组成，其中甲醇的作用是溶解美蓝和伊红以及固定细胞形态。
瑞氏染色液以瑞氏色素为主要原料，通过研磨配制而成，能呈现出清晰的细胞染色效果。该染液的特点：由瑞氏染色液和磷酸盐缓冲液组成，直接使用，无需任何配制过程，染液中加微量中性甘油，防止甲醇挥发或氧化，同时也可使血细胞染色较清晰。经常用于血液和细胞涂片、骨髓细胞涂片、细菌染色。细胞质呈红色，细胞核及细菌呈蓝色，嗜酸性颗粒呈橘红色。
| W743386 | Component | 2×100 mL | 2×500 mL | Storage |
| W743386A | 瑞氏染色液 | 100 mL | 500 mL | RT. Store in the dark |
| W743386B | 磷酸盐缓冲液 | 100 mL | 500 mL | RT. |
使用说明：
1、常规方法制备血液涂片或骨髓涂片或细菌涂片，待涂片自然干燥。
2、将血液涂片或骨髓涂片置于染色架上。
3、滴加适量瑞氏染色液覆盖涂片，室温染色1-2min。
4、涂片滴加等量磷酸盐缓冲液，轻轻晃动玻片或采用其他方式混合，使磷酸盐缓冲液与瑞氏染色液混匀，室温静置3-10min。
5、用自来水或蒸馏水从玻片一端轻轻冲洗。(注：也可先用磷酸盐缓冲液冲洗玻片，时间控制在30s左右)
6、干燥。
7、镜检：先用低倍镜观察血涂片，再用油镜。
注意事项：
1、血液涂片或骨髓涂片应厚薄均匀，以免影响染色效果。
2、涂片染色中，请勿先去除染液或直接对涂片用力冲洗。不能先倒掉染液，以免染料沉着于涂片上。
3、染色液可重复使用，但不能多次重复，若有沉淀物应过滤后使用。
4、染色过深可用甲醇或酒精适当脱色，最好不复染。
5、如果染色过深或过浅，应调整染色时间或工作液浓度。
6、pH 值对染色有一定影响，载玻片应清洁、无酸碱污染，以免影响染色效果。
7、为了您的安全和健康，请穿实验服并戴一次性手套操作。
染色结果：
| 细菌、细胞核 | 蓝色 |
| 嗜酸性颗粒 | 粉红或橘红色 |
| 淋巴细胞、嗜碱性粒细胞胞质 | 紫蓝色或蓝色 |
| 完全成熟红细胞 | 粉红色 |
Wright's stain is a compound dye consisting of the acidic dye Eosin and the basic dye Methylene Blue, which provides good differential staining for protoplasm. Different cellular components have distinct chemical properties and varying affinities to different dyes. For instance, hemoglobin and eosinophilic granules are alkaline proteins that bind with the acidic dye Eosin and stain pink, which are referred to as eosinophilic substances. Nuclear proteins, lymphocytes and the cytoplasm of basophilic granulocytes are acidic; they combine with the basic dye to stain purplish blue or blue and are called basophilic substances. Neutrophilic granules are at the isoelectric point and can bind both Eosin and Methylene Blue, staining pale magenta and named neutrophilic substances. The cytoplasm and nucleoli of pronormoblasts and early erythroblasts contain abundant acidic substances and stain deep blue. Intermediate erythroblasts contain both acidic and alkaline substances, staining reddish blue or grayish red. Fully mature red blood cells lose all acidic substances completely and stain pink. Wright's staining solution is a clear blue liquid with a characteristic methanol odor, mainly composed of Methylene Blue, Eosin and methanol. Methanol serves to dissolve Methylene Blue and Eosin as well as fix cell morphology.
Wright's staining solution is prepared by grinding with Wright's pigment as the main raw material, which can achieve clear staining results of cells.Characteristics of this staining solution: It is composed of Wright's staining solution and phosphate buffer solution, and can be used directly without any preparation. A trace amount of neutral glycerin is added into the staining solution to prevent methanol from volatilizing or oxidizing, and also make the staining of blood cells clearer. It is often used for staining blood smears, cell smears, bone marrow cell smears and bacteria. The cytoplasm is red, cell nuclei and bacteria are blue, and eosinophilic granules are orange-red.
Component List:
|
Instructions for Use:
1. Prepare blood smears, bone marrow smears or bacterial smears by conventional methods and let the smears air dry naturally.
2. Place the blood smears or bone marrow smears on a staining rack.
3. Drop an appropriate amount of Wright's staining solution to cover the smears and stain at room temperature for 2–3 minutes.
4. Add an equal volume of phosphate buffer solution onto the smears. Gently shake the slide or mix the liquids in other ways to fully blend phosphate buffer solution and Wright's staining solution, then let it stand at room temperature for 3–10 minutes.
5. Gently rinse the slide from one end with tap water or distilled water.
Note: Phosphate buffer solution can also be used to rinse the slide for about 30 seconds.
6. Air dry the slide.
7. Microscopic examination: Observe the blood smear under low-power lens first, then under oil immersion lens.
Precautions:
1. Blood smears and bone marrow smears shall be evenly spread in thickness to avoid affecting the staining effect.
2. Do not pour off the staining solution in advance or flush the slide hard during staining. Never pour out the staining solution first, otherwise dyes will precipitate and attach to the smear.
3. The staining solution can be reused but not repeatedly for many times. Filter the solution before reuse if precipitates appear.
4. Over-stained samples can be properly decolorized with methanol or ethanol; restaining is not recommended.
5. Adjust the staining time or working solution concentration if the staining result is too dark or too light.
6. pH value has a certain influence on staining. Slides must be clean and free of acid and alkali contamination to prevent poor staining results.
7. Wear a lab coat and disposable gloves during operation for your personal safety and health.
Staining Result
|
规格
别名
瑞氏染色液
英文别名
Wright Stain Solution
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
英文名称
Wright Staining Kit
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
Store at room temperature long term (24 months). Store in the dark.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333436 |
分析证书 | W743386 |
| ZJ25F1129673 |
分析证书 | W743386 |
| ZJ25F0521501 |
分析证书 | W743386 |
技术文档和文章
生物染色剂
目录： 阿拉丁试剂的规格、级别和纯度
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Eosinophil Morphological Observation: Staining Basis, Identification Points, and Research Validation
目录： 技术文章
溶液计算器
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）