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姬姆萨染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
姬姆萨色素 (又称吉姆萨色素) 是由天青Ⅱ与伊红混合而成，染色原理和结果与瑞氏染色基本相同，姬姆萨染色液对胞浆着色力较强，能较好的显示胞浆的嗜碱性程度，特别是对血液和骨髓细胞中的嗜天青、嗜酸性、嗜碱性颗粒，着色清晰，但是对胞核着色偏深，核结构显色不佳，故姬姆萨染液常与瑞氏染液联合使用。姬姆萨染色液以进口的姬姆萨色素、甲醇为主要原料，能呈现出清晰的细胞染色效果，经常用于组织切片、血液和细胞涂片、细菌、染色体显带、原生动物寄生虫等染色。嗜酸性颗粒为碱性蛋白质，与酸性染料伊红结合，染粉红色，称为嗜酸性物质；细胞核蛋白和淋巴细胞胞浆为酸性，与碱性染料美蓝或天青结合，染紫蓝色，称为嗜碱性物质；中性颗粒呈等电状态与伊红和美蓝均可结合，染淡紫色，称为中性物质。
本品由姬姆萨染色液 (10×) 和磷酸盐缓冲液组成，1：9混合成工作液后使用；亦可以分开使用，即先用姬姆萨染色液染色，再经磷酸盐缓冲液处理，亦可以得到满意的染色效果。
组分与储存：
| G743377 | Component | 100 mL | 500 mL | Storage |
| G743377A | 姬姆萨染色液 (10×) | 10 mL | 50 mL | RT. Store in the dark |
| G743377B | 磷酸盐缓冲液 | 100 mL | 500 mL | RT. |
使用说明：
（一）一步法涂片染色
1、姬姆萨工作液的配制：
按姬姆萨染色液 (10×): 磷酸盐缓冲液=1:9混合，即取1份姬姆萨染色液 (10×) 加入到9份的磷酸盐缓冲液中充分混匀，即为姬姆萨工作液，姬姆萨工作液为即用型试剂，不易保存，即用即配。
2、常规方法制备血液涂片或骨髓涂片，待涂片自然干燥后，用甲醇固定1-3min。
3、将血液涂片或骨髓涂片置于染色架上，滴加姬姆萨工作液覆盖涂片，室温滴染15-30min。
4、用自来水或蒸馏水缓慢从玻片一端冲洗。
5、干燥、镜检。
（二）两步法涂片染色
1、姬姆萨工作液的配制：
按姬姆萨染色液 (10×) : 蒸馏水=1:4混合，即取1份的姬姆萨染色液 (10×) 加入到4份的蒸馏水中充分混匀，即为姬姆萨工作液。姬姆萨工作液为即用型试剂，不易保存，即用即配。
2、常规方法制备血液涂片或骨髓涂片，待涂片自然干燥后，用甲醇固定1-3min。
3、将血液涂片或骨髓涂片置于染色架上，滴加适量姬姆萨工作液覆盖涂片，室温滴染10-15min。
4、加入等量磷酸盐缓冲液，轻轻晃动载玻片，室温静置5-10min。
5、用自来水或蒸馏水缓慢从玻片一端冲洗。
6、干燥、镜检。
（三）组织切片染色
1、姬姆萨工作液的配制：
按姬姆萨染色液 (10×): 磷酸盐缓冲液=1:9混合，即取1份姬姆萨染色液 (10×) 加入到9份的磷酸盐缓冲液中充分混匀，即为姬姆萨工作液，姬姆萨工作液为即用型试剂，不易保存，即用即配。
2、新鲜组织立即置于Regaud固定液固定2天，期间应更换1次固定液。
3、3%重铬酸钾固定1天。
4、流水冲洗16个小时或过夜。
5、照常规脱水、包埋。
6、切片厚度约为5μm，常规脱蜡至水。
7、蒸馏水清洗2次，每次1min。
8、入含姬姆萨工作液染缸，浸染18-24h。
9、蒸馏水稍微清洗。
10、0.1-0.5%乙酸洗1-2min。
11、自来水稍微冲洗。
12、用无水乙醇迅速脱水3次，每次5-10s。
13、二甲苯透明，中性树脂封固。
注意事项：
1、血液涂片或骨髓涂片应厚薄均匀，以免影响染色效果。
2、涂片染色中姬姆萨染色后，请勿先去除染液或直接对涂片用力冲洗。
3、如果染色过深或过浅，应调整染色时间或工作液浓度。
4、涂片染色和组织切片染色中，pH值对染色有一定影响，载玻片应清洁、无酸碱污染，以免影响染色效果。
5、染色液经稀释后液面应金属光泽则表示染液有染色作用，否则染色液可能失效。
6、组织切片染色中，染色后需用大量0.1-0.5%乙酸急速冲洗，避免浮面沉淀物污染切片后难以洗脱。
7、0.5%乙酸分化常用于姬姆萨组织切片染色，如有必要亦可用于细胞涂片，但其浓度应适量下调。0.5%乙酸分化切片时，切片呈粉红色即可终止。
8、姬姆萨组织切片染色中，无水乙醇脱水要迅速，否则切片易褪色。
9、涂片染色和组织切片染色中，如需急速获得结果，可按姬姆萨染色液 (10×): 磷酸盐缓冲液=1:1配制姬姆萨工作液，充分混匀，即为快速姬姆萨染色工作液，将染色液滴加于细胞涂片或组织切片上，加热染色，20-30s后重新加染色液，反复5-10次，其余步骤同上。
10、染色液可重复使用，但不能多次重复，若有沉淀物应过滤后使用。
11、Regaud固定液：按3%重铬酸钾: 甲醛=4:1配制，临用前混匀，1-2天后失效。
染色结果：
涂片染色：
| 嗜酸性颗粒 | 粉红色 |
| 嗜碱性颗粒 | 紫蓝色 |
| 中性颗粒 | 淡紫色 |
组织切片染色：
| 细胞核 | 蓝色至紫色 |
| 细胞质 | 淡蓝色 |
| 嗜铬细胞胞质 | 黄绿色 |
| 结缔组织 | 淡红色 |
Giemsa dye is composed of Azure II and eosin. Its staining principle and staining results are essentially similar to those of Wright staining. Giemsa Staining Solution has a strong affinity for the cytoplasm and can effectively demonstrate the degree of cytoplasmic basophilia, particularly providing clear staining of azurophilic, eosinophilic, and basophilic granules in blood and bone marrow cells. However, nuclear staining tends to be relatively intense, resulting in less distinct visualization of nuclear structures; therefore, Giemsa Staining Solution is often used in combination with Wright staining solution. Giemsa Staining Solution is mainly prepared from imported Giemsa dye and methanol and can produce clear cellular staining. It is commonly used for staining tissue sections, blood and cell smears, bacteria, chromosome banding preparations, protozoan parasites, and other specimens. Eosinophilic granules contain basic proteins that bind to the acidic dye eosin and are stained pink, and are therefore referred to as eosinophilic substances; nuclear proteins and the cytoplasm of lymphocytes are acidic and bind to the basic dyes methylene blue or azure, resulting in a purple-blue color, and are therefore referred to as basophilic substances; neutral granules are in an isoelectric state and can bind to both eosin and methylene blue, resulting in a light purple color, and are therefore referred to as neutrophilic substances.
This product consists of Giemsa Staining Solution (10×) and phosphate buffer. The two components are mixed at a ratio of 1:9 to prepare the working solution before use; alternatively, they may be used separately, that is, the specimen is first stained with Giemsa Staining Solution and then treated with phosphate buffer, which can also produce satisfactory staining results.
Contents & Storage:
| G743377 | Component | 100 mL | 500 mL | Storage |
| G743377A | Giemsa Staining Solution (10×) | 10 mL | 50 mL | Room temperature. Store in the dark. |
| G743377B | Phosphate Buffer | 100 mL | 500 mL | Room temperature. |
Instructions for Use:
(I) One-Step Smear Staining
1、Preparation of Giemsa working solution:
Mix Giemsa Staining Solution (10×) and phosphate buffer at a ratio of 1:9. Add 1 part of Giemsa Staining Solution (10×) to 9 parts of phosphate buffer and mix thoroughly to obtain the Giemsa working solution. The Giemsa working solution is a ready-to-use reagent and is not suitable for storage; prepare immediately before use.
2、Prepare a blood smear or bone marrow smear using a conventional method. Allow the smear to air-dry naturally, and then fix with methanol for 1–3 min.
3、Place the blood smear or bone marrow smear on a staining rack. Add Giemsa working solution dropwise to completely cover the smear and stain at room temperature for 15–30 min.
4、Rinse slowly with tap water or distilled water from one end of the slide.
5、Dry and examine microscopically.
(II) Two-Step Smear Staining
1、Preparation of Giemsa working solution:
Mix Giemsa Staining Solution (10×) and distilled water at a ratio of 1:4. Add 1 part of Giemsa Staining Solution (10×) to 4 parts of distilled water and mix thoroughly to obtain the Giemsa working solution. The Giemsa working solution is a ready-to-use reagent and is not suitable for storage; prepare immediately before use.
2、Prepare a blood smear or bone marrow smear using a conventional method. Allow the smear to air-dry naturally, and then fix with methanol for 1–3 min.
3、Place the blood smear or bone marrow smear on a staining rack. Add an appropriate amount of Giemsa working solution dropwise to completely cover the smear and stain at room temperature for 10–15 min.
4、Add an equal volume of phosphate buffer, gently agitate the slide, and allow it to stand at room temperature for 5–10 min.
5、Rinse slowly with tap water or distilled water from one end of the slide.
6、Dry and examine microscopically.
(III) Tissue Section Staining
1、Preparation of Giemsa working solution:
Mix Giemsa Staining Solution (10×) and phosphate buffer at a ratio of 1:9. Add 1 part of Giemsa Staining Solution (10×) to 9 parts of phosphate buffer and mix thoroughly to obtain the Giemsa working solution. The Giemsa working solution is a ready-to-use reagent and is not suitable for storage; prepare immediately before use.
2、Immediately place fresh tissue in Regaud fixative and fix for 2 days, replacing the fixative once during this period.
3、Fix in 3% potassium dichromate for 1 day.
4、Wash under running water for 16 h or overnight.
5、Dehydrate and embed according to conventional procedures.
6、Cut sections to a thickness of approximately 5 μm and routinely deparaffinize to water.
7、Wash twice with distilled water for 1 min each time.
8、Place the sections in a staining jar containing Giemsa working solution and stain by immersion for 18–24 h.
9、Rinse briefly with distilled water.
10、Wash with 0.1–0.5% acetic acid for 1–2 min.
11、Rinse briefly with tap water.
12、Rapidly dehydrate three times with absolute ethanol for 5–10 s each time.
13、Clear in xylene and mount with neutral resin.
Precautions:
1、Blood smears or bone marrow smears should be uniform in thickness to avoid affecting the staining results.
2、During smear staining, after Giemsa staining, do not first remove the staining solution or directly rinse the smear forcefully.
3、If the staining is too dark or too light, adjust the staining time or the concentration of the working solution.
4、During smear staining and tissue section staining, pH has a certain influence on the staining results. Slides should be clean and free from acidic or alkaline contamination to avoid affecting the staining results.
5、After dilution, a metallic sheen should be visible on the surface of the staining solution, indicating that the staining solution is active; otherwise, the staining solution may have lost its effectiveness.
6、During tissue section staining, after staining, rapidly rinse with a large amount of 0.1–0.5% acetic acid to prevent surface precipitates from contaminating the sections and becoming difficult to remove.
7、Differentiation with 0.5% acetic acid is commonly used for Giemsa staining of tissue sections and may also be used for cell smears if necessary, but the concentration should be appropriately reduced. When differentiating tissue sections with 0.5% acetic acid, stop the differentiation once the sections appear pink.
8、During Giemsa staining of tissue sections, dehydration with absolute ethanol should be performed rapidly; otherwise, the sections may fade easily.
9、During smear staining and tissue section staining, if rapid results are required, prepare the Giemsa working solution by mixing Giemsa Staining Solution (10×) and phosphate buffer at a ratio of 1:1 and mix thoroughly to obtain the rapid Giemsa staining working solution. Apply the staining solution dropwise to the cell smear or tissue section and heat during staining. After 20–30 s, add fresh staining solution again and repeat this procedure 5–10 times. The remaining steps are the same as described above.
10、The staining solution may be reused, but should not be reused repeatedly. If precipitates are present, filter the solution before use.
11、Regaud fixative: prepare by mixing 3% potassium dichromate and formaldehyde at a ratio of 4:1. Mix thoroughly immediately before use. The fixative loses effectiveness after 1–2 days.
Staining Results:
Smear Staining:
| Eosinophilic granules | Pink |
| Basophilic granules | Purple-blue |
| Neutral granules | Light purple |
Tissue Section Staining:
| Cell nuclei | Blue to purple |
| Cytoplasm | Light blue |
| Chromaffin cell cytoplasm | Yellow-green |
| Connective tissue | Light red |
规格
别名
姬姆萨染色液 (1:9) | 吉姆萨染色试剂盒
英文别名
Giemsa Staining Solution
规格或纯度
BioReagent, 生物染色剂, 用于显微镜, 10×
稳定性与储存
Store at room temperature long term (24 months). Store in the dark.
英文名称
Giemsa Staining Solution
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332501 |
分析证书 | G743377 |
| ZJ26F0332500 |
分析证书 | G743377 |
| ZJ25F1129924 |
分析证书 | G743377 |
| ZJ25F1129671 |
分析证书 | G743377 |
| ZJ25F0825127 |
分析证书 | G743377 |
| ZJ25F0825123 |
分析证书 | G743377 |
| ZJ25F0724320 |
分析证书 | G743377 |
| ZJ25F0521430 |
分析证书 | G743377 |
技术文档和文章
生物染色剂
目录： 阿拉丁试剂的规格、级别和纯度
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Eosinophil Morphological Observation: Staining Basis, Identification Points, and Research Validation
目录： 技术文章
Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy
目录： 技术文章
溶液计算器
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