计算溶液所需的质量、体积或浓度。
淀粉样物质染色液 (Highman刚果红法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
淀粉样物质是一种无固定形状的细胞外嗜酸性物质，可存在于不同的组织、器官，导致的疾病称为淀粉样变.淀粉样物质主要是由蛋白质构成，该蛋白大部分排列成反向的β-折叠层结构，在电子显微镜下淀粉样物质呈原纤维排列，病例材料中为大量细胞外的不分支的细丝，大多随机排列。用于识别淀粉样物质的组织学方法有甲紫染色、刚果红染色、偏振光显微镜观察等，目前研究发现传统的甲紫染色法灵敏度低、特异性差，经典的而且有效的方法是刚果红染色，1922年Bennhold发现了刚果红可以用于活体内淀粉样物质的鉴别，并应用到组织切片。后来经过Highman改良，染色效果更好。
淀粉样物质染色液 (Highman刚果红法)主要由刚果红染色液和苏木素染色液组成，该染色液简单易行，染色液性能稳定，并且已经被广泛应用。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
| A1508468 | Component | 3×50 mL | Storage |
| A1508468A | Highman刚果红染色液 | 50 mL | RT. Store in the dark. |
| A1508468B | 碱性乙醇分化液 | 50 mL | RT. |
| A1508468C | 苏木素染色液 | 50 mL | RT. Store in the dark. |
自备材料：
10%中性福尔马林、蒸馏水、系列乙醇、二甲苯或环保浸蜡脱蜡透明液、中性树胶
操作步骤(仅供参考)：
1、常规固定，常采用10%中性福尔马林固定液，常规脱水包埋。
2、石蜡切片二甲苯或脱蜡透明液脱蜡入蒸馏水；冰冻切片直接入蒸馏水；如有必要，可以去除色素。
3、入Highman刚果红染色液浸染5-10min。
4、碱性乙醇分化3-10s，立即入水终止分化，自来水冲洗。
5、入苏木素染色液，染细胞核1-2min。
6、自来水稍微冲洗，更换蒸馏水清洗，使其分化、返蓝。
7、逐级常规乙醇脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
淀粉样物质、弹力纤维、嗜伊红颗粒: 红色
细胞核: 蓝色
注意事项：
1、切片脱蜡应尽量干净，否则影响染色效果。
2、碱性乙醇分化液应密闭保存，一旦开启尽快用完。
3、分化时间应该根据切片厚薄、组织类别和分化液的新旧而定，另外分化后自来水冲洗时间应该足够。
4、碱性乙醇分化液分化步骤很重要，应及时入水终止分化，防止分化过度。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Amyloid is an amorphous extracellular eosinophilic substance that can be deposited in various tissues and organs, and the diseases caused thereby are referred to as amyloidosis. Amyloid is mainly composed of proteins, most of which are arranged in an antiparallel β-pleated sheet structure. Under an electron microscope, amyloid presents a fibrillary arrangement, appearing as a large number of unbranched extracellular filaments in pathological specimens, mostly arranged randomly. Histological methods for identifying amyloid include methyl violet staining, Congo red staining, and polarized light microscopy. Current research has demonstrated that the traditional methyl violet staining method has low sensitivity and poor specificity, whereas Congo red staining is a classic and effective approach. In 1922, Bennhold discovered that Congo red could be used for the identification of amyloid in vivo and applied this technique to tissue sections. Later, the method was modified by Highman, resulting in improved staining efficacy.
Amyloid Staining Solution (Highman Congo Red Method) is mainly composed of Highman Congo Red Staining Solution and Hematoxylin Staining Solution. This staining kit is simple to operate, features stable performance, and has been widely used in relevant research. This reagent is for research use only and is not suitable for clinical diagnosis or any other purposes.
| A1508468 | Component | 3×50 mL | Storage |
| A1508468A | Highman Congo Red Staining Solution | 50 mL | RT. Store in the dark. |
| A1508468B | Alkaline Ethanol Differentiating Solution | 50 mL | RT. |
| A1508468C | Hematoxylin Staining Solution | 50 mL | RT. Store in the dark. |
Materials to be Prepared by User
10% neutral formalin, distilled water, graded ethanol, xylene or environment-friendly paraffin removal and clearing solution, neutral balsam
Procedure (for reference only)
1. Perform routine fixation, usually with 10% neutral formalin fixative, followed by routine dehydration and embedding.
2. Dewax paraffin sections with xylene or dewaxing and clearing solution and rinse them in distilled water; directly immerse frozen sections in distilled water. If necessary, remove pigments from the sections.
3. Immerse the sections in Highman Congo Red Staining Solution for 5-10 min.
4. Differentiate with Alkaline Ethanol Differentiating Solution for 3-10 s, immediately transfer the sections to water to terminate differentiation, and rinse with tap water.
5. Immerse the sections in Hematoxylin Staining Solution to stain cell nuclei for 1-2 min.
6. Rinse briefly with tap water, then transfer to distilled water for rinsing to achieve differentiation and bluing.
7. Conduct routine dehydration with graded ethanol, clear the sections with xylene or dewaxing and clearing solution, and mount them with neutral balsam.
Staining Results
Amyloid, elastic fibers, eosinophilic granules: Red
Cell nucleus: Blue
Precautions
1. Ensure thorough dewaxing of sections; otherwise, the staining effect will be compromised.
2. Store Alkaline Ethanol Differentiating Solution in a sealed container and use it as soon as possible after opening.
3. The differentiation time should be determined based on section thickness, tissue type, and the freshness of the differentiating solution. In addition, ensure sufficient rinsing time with tap water after differentiation.
4. The differentiation step with Alkaline Ethanol Differentiating Solution is critical. Immediately transfer the sections to water to terminate differentiation to prevent over-differentiation.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. Use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
规格
别名
淀粉样物质染色液
英文别名
Amyloid Staining Solution
规格或纯度
BioReagent,用于显微镜,生物染色剂
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Amyloid Staining Solution (Highman Congo Red Method)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332728 |
分析证书 | A1508468 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
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每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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