计算溶液所需的质量、体积或浓度。
淀粉样物质染色液 (Bennhold刚果红法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
淀粉样物质是一种无固定形状的细胞外嗜酸性物质，可存在于不同的组织、器官，导致的疾病称为淀粉样变，淀粉样物质主要是由蛋白质构成，该蛋白大部分排列成反向的β-折叠层结构，在电子显微镜下淀粉样物质呈原纤维排列，病例材料中为大量细胞外的不分支的细丝，大多随机排列；用于识别淀粉样物质的组织学方法有甲紫染色、刚果红染色、偏振光显微镜观察等。目前研究发现传统的甲紫染色法灵敏度低、特异性差，经典的而且有效的方法是刚果红染色，1922年Bennhold发现了刚果红可以用于活体内淀粉样物质的鉴别，并应用到组织切片。
淀粉样物质染色液 (Bennhold刚果红法)主要由刚果红染色液、苏木素染色液等组成，其染色原理在于淀粉样物质对刚果红比其他的组织结构具有更大的亲和力，其羟基与刚果红的氨基结合，从而使淀粉样物质染成红色，该染色法性能稳定，是非常经典的淀粉样物质染色的方法。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
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自备材料：
10%中性福尔马林固定液、蒸馏水、系列乙醇、二甲苯或环保浸蜡脱蜡透明液
操作步骤(仅供参考)：
1、常规固定，常采用10%中性福尔马林固定液，常规脱水包埋。
2、切片厚度4μm，常规二甲苯或脱蜡透明液脱蜡至水。
3、入Bennhold苏木素染色液浸染5min。
4、酸性乙醇分化2-5s，立即入水终止分化，水洗2次后镜下控制至恰当程度。
5、自来水冲洗2min。
6、入Scott蓝化液返蓝，自来水冲洗2min。
7、入刚果红染色液浸染30min。
8、Bennhold分化液迅速分化15s。
9、放入新配制的80%乙醇分化至红色不褪为止，自来水冲洗1-2min。
10、逐级常规乙醇脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
淀粉样物质: 红色
细胞核: 蓝色
注: 在偏光显微镜下，淀粉样物质呈黄绿色的双折光。
注意事项：
1、切片脱蜡应尽量干净，否则影响染色效果。
2、酸性乙醇分化液应密闭保存，一旦开启尽快用完。
3、刚果红染色液染色时尽量采用浸染，如果滴染，应置于湿盒防止溶液挥发。
4、Bennhold分化液分化步骤很重要，应随时注意分化程度，至无多余染料流下为止；分化时间较短，胶原纤维也被染成红色；分化过度，淀粉样物质也被脱色；如果脱色过度，可以将切片清洗后重新用刚果红染色液浸染。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Amyloid is an amorphous extracellular eosinophilic substance that can be present in various tissues and organs. The diseases caused by it are called amyloidosis. Amyloid is mainly composed of proteins, most of which are arranged in antiparallel β-pleated sheet structures. Under an electron microscope, amyloid appears as a fibrillary arrangement, presenting as a large number of unbranched extracellular filaments in pathological specimens, mostly arranged randomly. Histological methods for identifying amyloid include methyl violet staining, Congo red staining, and polarized light microscopy. Current research has found that the traditional methyl violet staining method has low sensitivity and poor specificity, while the classic and effective method is Congo red staining. In 1922, Bennhold discovered that Congo red could be used for the identification of amyloid in vivo and applied it to tissue sections.
Amyloid Staining Solution (Bennhold Congo Red Method) is mainly composed of Congo Red Staining Solution, Hematoxylin Staining Solution, etc. Its staining principle lies in that amyloid has a higher affinity for Congo red than other tissue structures, and its hydroxyl groups combine with the amino groups of Congo red, thus staining amyloid red. This staining method has stable performance and is a very classic method for amyloid staining. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
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Materials to be Prepared by User
10% neutral formalin fixative, distilled water, graded ethanol, xylene or environment-friendly paraffin removal and clearing solution
Procedure (for reference only)
1. Routine fixation, usually with 10% neutral formalin fixative, followed by routine dehydration and embedding.
2. Cut sections with a thickness of 4 μm, and perform routine dewaxing to water using xylene or dewaxing and clearing solution.
3. Immerse in Bennhold Hematoxylin Staining Solution for 5 min.
4. Differentiate with Acid Ethanol Differentiating Solution for 2-5 s, immediately transfer to water to terminate differentiation, wash twice with water, and check under a microscope to ensure appropriate differentiation.
5. Rinse with tap water for 2 min.
6. Immerse in Scott's Bluing Solution for bluing, then rinse with tap water for 2 min.
7. Immerse in Congo Red Staining Solution for 30 min.
8. Rapidly differentiate with Bennhold Differentiating Solution for 15 s.
9. Transfer to freshly prepared 80% ethanol and differentiate until the red color is stable without fading, then rinse with tap water for 1-2 min.
10. Perform routine dehydration with graded ethanol, clear with xylene or dewaxing and clearing solution, and mount with neutral balsam.
Staining Results
Amyloid: Red
Cell Nucleus: Blue
Note: Under a polarized light microscope, amyloid exhibits yellowish-green birefringence.
Precautions
1. Ensure thorough dewaxing of sections; otherwise, the staining effect will be affected.
2. Store Acid Ethanol Differentiating Solution in a sealed container and use it as soon as possible after opening.
3. Prefer immersion staining when using Congo Red Staining Solution; if drop staining is adopted, place the sections in a moist chamber to prevent solution evaporation.
4. The differentiation step with Bennhold Differentiating Solution is critical. Always monitor the differentiation degree until no excess dye drips off. Short differentiation time will result in collagen fibers being stained red; over-differentiation will cause amyloid to decolorize. If over-decolorization occurs, rinse the sections and re-stain with Congo Red Staining Solution.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
淀粉样物质染色液
英文别名
Amyloid Staining Solution
规格或纯度
BioReagent,用于显微镜,生物染色剂
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Amyloid Staining Solution (Bennhold Congo Red Method)
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
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C of A & Other Certificates(BSE/TSE, COO):
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332657 |
分析证书 | A1508461 |
技术文档和文章
溶液计算器
常见问题
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
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