FABP4 Mouse mAb

    应用:
  • IHC
  • WB
功能和特点
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 猪(Pig)
  • 亚型: Mouse IgG1
  • 克隆号: C12
  • 偶联: Unconjugated
有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab102606-10μl
10μl 现货 Stock Image
Ab102606-50μl
50μL 现货 Stock Image
Ab102606-100μl
100μL 期货 Stock Image
Ab102606-1ml
1ml 期货 Stock Image

基本信息

产品名称 FABP4 Mouse mAb
别名 脂肪酸结合蛋白4单克隆抗体 | FABP4 小鼠单克隆抗体 | FABP4 小鼠单抗
英文别名 3T3-L1 lipid-binding protein antibody | 422/aP2 antibody | A-FABP antibody | adipocyte antibody | Adipocyte lipid binding protein antibody | Adipocyte lipid-binding protein antibody | Adipocyte protein AP2 antibody | Adipocyte-type fatty acid-binding prot
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
特异性 FABP4
种属反应性 人(Human),大鼠(Rat),猪(Pig)
免疫原 Recombinant FABP4 expressed in E.coli (AA 2-132)
阳性对照 WB: Mouse heart, Mouse skeletal muscle tissue lysates and HUVEC cell lysate. IHC: Human and Porcine cardiac muscle tissues.
偶联 Unconjugated

AI解读

产品属性

克隆类型 单克隆抗体
克隆号 C12
Format Whole IgG
亚型 Mouse IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A + Protein G purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂 0.05% Proclin 300
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

FABP4 Mouse mAb (Ab102606) - Western Blot
All lanes: FABP4 Mouse mAb (Ab102606) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa
Exposure time: 0.9 s


FABP4 Mouse mAb (Ab102606) - Western Blot
All lanes: FABP4 Mouse mAb (Ab102606) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 14 kDa
Exposure time: 159.3 s

FABP4 Mouse mAb (Ab102606) - IHC
DAB staining on IHC
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 10 µg /mL FABP4 Mouse mAb (Ab102606)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

FABP4 Mouse mAb (Ab102606) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 10 µg/mL FABP4 Mouse mAb (Ab102606)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

关联靶点(人)

FABP4 Tchem 脂肪酸结合蛋白、脂肪细胞(Fatty acid-binding protein, adipocyte) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

应用

应用名称 稀释比例
IHC

5-20 µg/mL

WB

0.01-2 µg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到3个结果

批号(Lot Number) 证书类型 日期 货号
ZJ24F0102397 分析证书 24-01-10 Ab102606
ZJ24F0102396 分析证书 24-01-10 Ab102606
ZJ24F0102395 分析证书 24-01-10 Ab102606

参考文献

1. Hertzel AV, Hellberg K, Reynolds JM, Kruse AC, Juhlmann BE, Smith AJ, Sanders MA, Ohlendorf DH, Suttles J, Bernlohr DA.  (2009)  Identification and characterization of a small molecule inhibitor of Fatty Acid binding proteins..  J Med Chem,  52  (19): (6024-31).  [PMID:19754198] [10.1021/op500134e]
2. Liu X, Huang X, Lin W, Wang D, Diao Y, Li H, Hui X, Wang Y, Xu A, Wu D et al..  (2011)  New aromatic substituted pyrazoles as selective inhibitors of human adipocyte fatty acid-binding protein..  Bioorg Med Chem Lett,  21  (10): (2949-52).  [PMID:21481589] [10.1021/op500134e]
3. Oddi S, Scipioni L, Totaro A, Angelucci C, Dufrusine B, Sabatucci A, Tortolani D, Coletta I, Alisi MA, Polenzani L et al..  (2019)  The anti-inflammatory agent bindarit acts as a modulator of fatty acid-binding protein 4 in human monocytic cells..  Sci Rep,  (1): (15155).  [PMID:31641194] [10.1021/op500134e]
4. Haiyan Cai,Guirui Yan,Xiaodong Zhang,Olena Gorbenko,Heyao Wang,Weiliang Zhu.  (2010-05-18)  Discovery of highly selective inhibitors of human fatty acid binding protein 4 (FABP4) by virtual screening..  Bioorganic & medicinal chemistry letters,  20  ((12)): (3675-3679).  [PMID:20471252]
5. Lehmann F, Haile S, Axen E, Medina C, Uppenberg J, Svensson S, Lundbäck T, Rondahl L, Barf T..  (2004)  Discovery of inhibitors of human adipocyte fatty acid-binding protein, a potential type 2 diabetes target..  Bioorg Med Chem Lett,  14  (17): (4445-4448).  [PMID:15357969] [10.1016/j.bmcl.2004.06.057]
6. Barf T, Lehmann F, Hammer K, Haile S, Axen E, Medina C, Uppenberg J, Svensson S, Rondahl L, Lundbäck T..  (2009)  N-Benzyl-indolo carboxylic acids: Design and synthesis of potent and selective adipocyte fatty-acid binding protein (A-FABP) inhibitors..  Bioorg Med Chem Lett,  19  (6): (1745-1748).  [PMID:19217286] [10.1016/j.bmcl.2009.01.084]
7. Cai H, Liu Q, Gao D, Wang T, Chen T, Yan G, Chen K, Xu Y, Wang H, Li Y, Zhu W..  (2015)  Novel fatty acid binding protein 4 (FABP4) inhibitors: virtual screening, synthesis and crystal structure determination..  Eur J Med Chem,  90  (241-250).  [PMID:25461324] [10.1016/j.ejmech.2014.11.020]
8. Zhou Y, Nie T, Zhang Y, Song M, Li K, Ding M, Ding K, Wu D, Xu Y..  (2016)  The discovery of novel and selective fatty acid binding protein 4 inhibitors by virtual screening and biological evaluation..  Bioorg Med Chem,  24  (18): (4310-4317).  [PMID:27460668] [10.1016/j.bmc.2016.07.022]
9. Kühne H, Obst-Sander U, Kuhn B, Conte A, Ceccarelli SM, Neidhart W, Rudolph MG, Ottaviani G, Gasser R, So SS, Li S, Zhang X, Gao L, Myers M..  (2016)  Design and synthesis of selective, dual fatty acid binding protein 4 and 5 inhibitors..  Bioorg Med Chem Lett,  26  (20): (5092-5097).  [PMID:27658368] [10.1016/j.bmcl.2016.08.071]
10. Gao DD, Dou HX, Su HX, Zhang MM, Wang T, Liu QF, Cai HY, Ding HP, Yang Z, Zhu WL, Xu YC, Wang HY, Li YX..  (2018)  From hit to lead: Structure-based discovery of naphthalene-1-sulfonamide derivatives as potent and selective inhibitors of fatty acid binding protein 4..  Eur J Med Chem,  154  (44-59).  [PMID:29775936] [10.1016/j.ejmech.2018.05.007]
11. Yan S, Elmes MW, Tong S, Hu K, Awwa M, Teng GYH, Jing Y, Freitag M, Gan Q, Clement T, Wei L, Sweeney JM, Joseph OM, Che J, Carbonetti GS, Wang L, Bogdan DM, Falcone J, Smietalo N, Zhou Y, Ralph B, Hsu HC, Li H, Rizzo RC, Deutsch DG, Kaczocha M, Ojima I..  (2018)  SAR studies on truxillic acid mono esters as a new class of antinociceptive agents targeting fatty acid binding proteins..  Eur J Med Chem,  154  (233-252).  [PMID:29803996] [10.1016/j.ejmech.2018.04.050]
12. He Y, Dou H, Gao D, Wang T, Zhang M, Wang H, Li Y..  (2019)  Identification of new dual FABP4/5 inhibitors based on a naphthalene-1-sulfonamide FABP4 inhibitor..  Bioorg Med Chem,  27  (19): (115015-115015).  [PMID:31420256] [10.1016/j.bmc.2019.07.031]
13. Oukoloff K, Lucero B, Francisco KR, Brunden KR, Ballatore C..  (2019)  1,2,4-Triazolo[1,5-a]pyrimidines in drug design..  Eur J Med Chem,  165  (332-346).  [PMID:30703745] [10.1016/j.ejmech.2019.01.027]
14. Baxa, C A CA and 7 more authors..  (1989)  Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA..  Biochemistry,  (31): [PMID:2481498]
15. Sjöblom, Tobias T and 28 more authors..  (2006)  The consensus coding sequences of human breast and colorectal cancers..  Science (New York, N.Y.),  (13): [PMID:16959974]
16. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,  [PMID:22223895]
17. Rosenow, Anja A and 6 more authors..  (2012)  Resveratrol-induced changes of the human adipocyte secretion profile..  Journal of proteome research,  (7): [PMID:22905912]

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