Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
Post-translational: Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3. Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation. ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
宿主种属
兔(Rabbit)
特异性
IRF3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human IRF3 (AA 82-123).
Recombinant IRF3 Antibody (Ab111210) - Western Blot All lanes: Recombinant IRF3 Antibody (Ab111210) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa Observed band size: 49 kDa Exposure time: 39.7 s
Recombinant IRF3 Antibody (Ab111210) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant IRF3 Antibody (Ab111210) at 1/50 dilution.
Recombinant IRF3 Antibody (Ab111210) - IF/ICC Immunofluorescent analysis of Jurkat cells, using Recombinant IRF3 Antibody (Ab111210).
Recombinant IRF3 Antibody (Ab111210) - IHC Immunohistochemical analysis of paraffin-embedded Human Hodgkin’s lymphoma, using Recombinant IRF3 Antibody (Ab111210) at 1/100 dilution.
Recombinant IRF3 Antibody (Ab111210) - IHC Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using Recombinant IRF3 Antibody (Ab111210) at 1/100 dilution.
Recombinant IRF3 Antibody (Ab111210) - IHC Immunohistochemical analysis of paraffin-embedded Human ovarian cancer, using Recombinant IRF3 Antibody (Ab111210) at 1/100 dilution.
Recombinant IRF3 Antibody (Ab111210) - IHC Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using Recombinant IRF3 Antibody (Ab111210).
ZJ25F0723910
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