ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse anti Human Mre11 Antibody, Monoclonal antibody, could be used for ELISA, WB, Flow, IHC and so on.
Application:
WB: 1/500 - 1/2000. Predicted molecular weight: 80.6 kDa.
IHC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000
Protein Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR.
Mre11 Mouse mAb (Ab115912) - Western Blot
All lanes: Mre11 Mouse mAb (Ab115912) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 80.6 kDa
Observed band size: 84.2 kDa
Mre11 Mouse mAb (Ab115912) - Flow Cytometry
Flow cytometric analysis of cell line labeling Mre11 with Mre11 Mouse mAb (Ab115912) (green) compared with a negative control (red).
Mre11 Mouse mAb (Ab115912) - Flow Cytometry
Flow cytometric analysis of K562 cell line labeling Mre11 with Mre11 Mouse mAb (Ab115912) (green) compared with a negative control (red).
Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.
Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.
Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse testis tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.
Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded Rat testis tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.
Mre11 Mouse mAb (Ab115912) - IHC
Immunohistochemical analysis of paraffin-embedded Rabbit testis tissues using Mre11 Mouse mAb (Ab115912) with DAB staining.
1/200-1/1000
1/200-1/400
1/10000
1/500-1/2000.Predicted molecular weight:80.6 kDa.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F0800754 |
分析证书 | Ab115912 |
| ZJ23F0800755 |
分析证书 | Ab115912 |
| ZJ24F1012045 |
分析证书 | Ab115912 |
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