ExactAb™,PBS Only,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for WB, ELISA, CLIA, LF, GICA and so on.
WB: 1/400 - 1/2000
ELISA : 1/5000 - 1/15000
LF/ GICA : 0.5-1.0mg/ml
Function
IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) - ELISA
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) was tested by ELISA. The plate was coated with different amounts of Rabbit IgG (Ab170215) (3-fold started from 333 ng/mL). Goat Anti-Rabbit IgG H&L Antibody was then added at a series of different dilution ratios. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
Goat Anti-Rabbit IgG H&L Antibody (Ab138272) - ELISA
Cross-reactivity of Goat Anti-Rabbit IgG H&L Antibody (Ab138272) was tested by ELISA. The wells were coated with the indicated IgG standards at 0.5 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Rabbit IgG H&L Antibody was then added at different dilution ratio followed by incubation for 1h. Rabbit Anti-Goat IgG H&L (HRP) (Ab141534) was used at 1/10000 dilution as the secondary antibody.
For the batch tested, Goat Anti-Rabbit IgG H&L Antibody (Ab138272) showed a cross-reactivity with Human IgG when the dilution ratio of Goat Anti-Rabbit IgG H&L Antibody is less than 1/8000.
1/400 - 1/2000
1/5000 - 1/15000
0.5-1.0 mg/mL
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1215914 |
分析证书 | Ab138272 |
| ZJ24F1215915 |
分析证书 | Ab138272 |
| ZJ25F0522010 |
分析证书 | Ab138272 |
| ZJ25F0522009 |
分析证书 | Ab138272 |
| ZJ25F0522008 |
分析证书 | Ab138272 |
| ZJ23F0800779 |
分析证书 | Ab138272 |
| ZJ23F0800780 |
分析证书 | Ab138272 |
| ZJ23F0800781 |
分析证书 | Ab138272 |
| ZJ24F1215913 |
分析证书 | Ab138272 |
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