Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.
Liquid in PBS pH 7.4, containing 1% BSA and 0.1% Proclin300.
防腐剂
0.1% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.
图片
Goat Anti-Rabbit IgG H&L (AF647) (Ready to use) (Ab218696) - IF/ICC IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ready to use) (Ab218696) at 2mL/well for 1 hour at room temperature in the dark (red). A 6-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
ZJ25F0826278
危险品化学品经营许可证（带存储）