Donkey Anti-Rabbit IgG H&L (AF647)

已验证

ExactAb™

无叠氮钠

Ex:650nm

Em:668nm

2.0mg/ml

应用:

Flow Cytometry
IF/ICC

功能和特点

宿主种属: 驴(Donkey)
种属反应性: 兔(Rabbit)
亚型: Donkey IgG
偶联: AF647

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab136882-100μg	100μg	现货
Ab136882-1mg	1mg	期货
Ab136882-500μg	500μg	现货

查看相关系列

AF647 (110) Donkey Anti-Rabbit (2) 流式细胞术 (0)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本信息

产品名称	Donkey Anti-Rabbit IgG H&L (AF647)
英文别名	Donkey Anti-Rabbit IgG H&L / AF647 \| Immunoglobulin G \| Donkey Anti-Rabbit IgG \| Donkey Anti Rabbit IgG
规格或纯度	ExactAb™, 已验证, 无叠氮钠, Ex:650nm, Em:668nm, 2.0mg/ml
宿主种属	驴(Donkey)
种属反应性	兔(Rabbit)
免疫原	Native rabbit IgG
阳性对照	IF/ICC: Mouse cerebellum; Mouse brain; Human brain; Rat cerebellum; Rat brain
偶联	AF647
Excitation(nm)	Red Laser(633nm)
Emission(nm)	668nm
Ex/Em(nm)	Ex:650nm;Em:668nm

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	IgG
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10 mM TBS (pH=7.4) with 1% BSA, 0.03% ProClin300 and 50% Glycerol
防腐剂	0.03% ProClin300
浓度	2.0mg/ml
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在-20℃下可保存一年。避免反复冻融循环
分子类型	抗体

图片

IF: Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882)

Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IF
IF analysis of HeLa cells labeling FXR1 with purified Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

IHC: Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882)

Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min.Blocking buffer (normal goat serum) at 37°C for 30min. Antibody incubation with (GFAP) polyclonal antibody at 1/200 overnight at 4°C, followed by a conjugated Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) for 90 minutes, and DAPI for nuclei staining.