WB: HeLa, Jurkat, HepG2, K562, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate ICC/IF: U-2 OS cells
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
I01/6C1
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
图片
SOD2 Mouse mAb (Ab169025) - Western Blot All lanes: SOD2 Mouse Antibody (Ab169025) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 38.2 s
SOD2 Mouse mAb (Ab169025) - Western Blot All lanes: SOD2 Mouse Antibody (Ab169025) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 38.2 s
SOD2 Mouse mAb (Ab169025) - ICC/IF IF analysis of SOD2 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with SOD2 Mouse mAb (Ab169025) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.