Mouse anti Human PLGF Antibody, Monoclonal (5D9-3), is a Capture or Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA. CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE FLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176388) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176386) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176388) ·Detection antibody: PLGF Mouse mAb (Ab176386)
Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176386) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176388) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176386) ·Detection antibody: PLGF Mouse mAb (Ab176388)
Binding ELISA - PLGF Mouse mAb (Ab176388) Immobilized Recombinant Human PLGF Protein at 1.0 μg/mL can bind PLGF Mouse mAb (Ab176388) with the EC₅₀ of 38.54 ng/mL.
应用
应用名称
稀释比例
CLIA
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
ZJ23F1101535
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